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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

In order to overcome the shortage of conformable precision, polyamide affinity membrane modified by cibacron blue F3GA was introduced into the pretreatment unit to get rid of interferer from large molecule such as albumin in serum. The affinity membrane was combined with micro-fluidic chip by embedded and external methods and using external-combination pretreatment unit, average enrichment was 89.36 times, RSD was 4.37%.

为了提高测试的精密度，进而设计含膜复合式预处理芯片，探讨了不同预处理单元对血清样品中痕量盐酸多巴酚丁胺富集效率的影响，优化设计了外接式硅胶整体柱－亲和膜微芯片固相萃取预处理单元，复合式的反相硅胶整体柱对血清中微量盐酸多巴酚丁胺的平均富集倍数提高到89.4，RSD为4.37%。

The conversion boundary of self-incompatibility was researched by sowing by stage. Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃.HE97 was primarily regard as self-incompatible line in this research. The method was by inbreeding and sib HE97, Half the silk of HE97 and Zheng58. Inbreeding and cross have been done simultaneously. The extending condition of pollen tube was observed by dying the silk after pollinating 4 hours. The individuals with pollen tube were observed having seeds, while the individuals without pollen tube were observed having few seeds or no seeds. So this study regard HE97 as sporophytic self-incompatibility .The planting ration of female parent HE97 and male parent 87-1 was 4:1.The nature cross-pollination was studied by the theory of endosperm xenity, the study show that the setting percentage of HE97 was 625%, in which the seed in self was 70% and the seed in hybrid was 30%. This was consistent with the study of silk anatomy structure, then come to the same conclusion: compatible pollen germination and fertilization can stimulate the self-incompatible pollen germination and fertilization in the same corn cob.

通过分期播种，进行了光温敏自交不亲和材料HE97亲和性转换区间的研究，依据田间跟踪调查叶龄及室内考种研究了HE97亲和性转换区间的光温临界值，结果表明：1日最低温度是影响HE97自交不亲和性的主要原因，亲和性转换区间在16.5℃～18.1℃之间。2通过HE97自交和姊妹交以及将HE97与郑58作为双亲，各自的花丝一分为二，分别正反交，授粉后4个小时分别取其花丝进行固定、染色，观察花粉管的伸长情况，根据结实率与花粉管伸长的对应关系，结果发现观察到花粉管的对应植株收获到了种子，而没有观察到花粉管的对应植株结实率极低或没有结实，本研究把HE97初步划为孢子体自交不亲和类型。3以HE97为母本，87-1为父本，按4母：1父行比，通过花粉直感效应研究天然异交率，结果母本HE97结实良好(62%)，其中自交粒占70%，异交粒占30%，结合花丝切片观察表明，亲和花粉的萌发及受精会导致落在同一果穗柱头上不亲和花粉的萌发不再受抑制。

Monovalent cations decrease the affinity of Mg〓 to the enzyme but don't affect the affinity of Fru-1, 6-P〓.

钾离子的存在使得酶对镁离子的亲和力下降，而对酶与底物的亲和力没有影响。

Change of sodium pump activity is involved in hypoxic injury in rat cortical neurons. High affinity sodium pump is re lated to the protection of hypoxic injury and low affinity sodium pump is related to the hypoxic injury.

Na，K-ATP酶活性改变参与了皮质神经元的缺氧性损伤，其中高汞和力Na，K-ATP酶与皮质神经元缺氧性损伤保护作用有关，而低亲和力Na，K-ATP酶则与其缺氧性损伤有关。

Stressing on "fine reputation","quality","service","credit","practice", not only the grandeur.④Encouraging the innovation, not the unearthliness, playing down the effect of entertainment stars or idols, decreasing the fashion index.⑤"personality" is not the "self central". Social sense of responsibility and interpersonal "feeling of affinity" are both intensified.3.1.2 Factor analysis, revealing 19 value element vectors, which include "comfort","personality","simple","fine reputation","responsibility","service","price","wisdom","distinction","enthusiasm","stimulation","taste","sex difference","quality","vigor","idol","fashion","affinity","aesthetic" and so on. Four orientation diagrams coordinated with the average factors and the standard deviations of these 19 value elements, described the multi directional value orientation structure for China college students' sportswear consumption.3.1.3 Clustering analysis are applied to classify the former 19 value elements, which discovered that three inferior group are classified from the college students' consumption group based on the whole specimen data. It could be the reference of enterprise brand building for the orientation of consumption values.3.2 General personal values, self conception and demographic are lead into as three major variables for the interrelationship analysis of sportswear consumption value system.3.2.1 Firstly, the college students having their general values and existing the inferior group feature regarding to the consumption values.

三、研究的主要成果及其意义对中国大学生休闲服消费价值体系进行探索性分析1、首先对中国大学生休闲服消费价值观量化问卷所调查的样本数据进行频数统计趋向性分析，发现呈现的主要特点有：(1)"个性化"和"简约"已成为潮流，而寻"刺激"、找"激情"总体收敛；(2)追求"美感"，更不放弃"舒适"，两者兼得才是完美；(3)重"美誉"、讲"质量"，重"服务"、讲"信用"，求实际、不求高贵，"明智"有素；(4)求新不求怪异，明星、偶像效应淡化、时尚指数不高；(5)"个性化"不是"唯我化"，社会责任感和人际"亲和力"在强化。2、其次对样本数据进行因子分析，揭示出以"舒适"、"个性"、"简约"、"美誉"、"责任"、"服务"、"价格"、"明智"、"独特"、"激情"、"刺激"、"品位"、"性差"、"质量"、"活力"、"偶像"、"时尚"、"亲和"和"美感"等十九个价值元素为矢量的休闲服消费价值体系，并以因子均值和标准差为参数勾画出19个价值元素坐标四方位图，从总体上描述了中国大学生休闲服消费多维的价值取向结构。3、再者进行聚类分析，对上述十九个休闲服消费价值元素作不同量值的分类，发现在样本总量中可以细分为有较大区分度的三个类别，呈现出大学生消费群体中存在休闲服消费价值体系不同的亚群体，可为以消费价值观为取向的企业品牌价值定位提供参考基谱。

The selectivity for K over Na of the low affinity K uptake system in the roots of Puccinellia tenuiflora was approximately similar to that in glycophytes. However, the selectivity for K over Na of high affinity K uptake system in the roots of Puccinellia tenuiflora was much stronger, which indicated that HAK and HKT played very significant roles in the process.

小花碱茅根低亲和性K 吸收系统对K 、Na 的选择性吸收能力与甜土植物大致相同；但其高亲和性K 吸收系统的选择性能力很强，表明HAK和HKT在此过程中起重要的作用。

The macrocyclic polyamines are a kind of excellent ligands and have a substantially high binding affinity with transition metal ions. Macrocyclic polyamine metal complexes as enzyme models are widely used in chemical biology, medicine and gene therapy. 1, 4, 7, 10-tetrazacyclododecane is a representative macrocyclic polyamines compound and shows high binding affinity with transition metal ions, such as Zn, Cu and Co. Their metal complexes especially multinuclear complexes were widely used in DNA, RNA recognition.

大环多胺化合物是一类性质独特的优秀配体，具有良好的金属配位能力，其金属配合物作为人工酶模型广泛应用于核酸结构及功能研究、基因表达与调控、抗癌药物的研制等生物、化学和医学领域。1，4，7，10-四氮杂十二烷是典型的大环多胺化合物，对过渡金属离子Zn， Cu and Co有良好的配位能力，这些金属配合物特别是多核金属配合物被广泛应用于DNA识别中。

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中，我们发现，除非过量，否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象；过量的La〓（La〓/CaM摩尔比＞4）将引起钙调蛋白结合功能的下降，同时明显改变金属-钙调蛋白-Mas三元复合物的构象；La〓对Ca〓CaM-Mas的N末端表现高度选择性，显示了在三元复合物中两种离子间的协同效应；在三元体系中，La〓与钙调蛋白的亲和力明显上升，而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异，显示了作用的选择性；Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序，La〓很可能优先与C末端结合，从而使Mas/Mas X首先与C末端结合，该顺序与钙离子相同；La〓的参与使三元复合物在动力学上更加稳定。

Affinity chromatography materials for kinase;2. To search a suitable process route of purification of kinase in yeast by affinity chromatography, hexokinase was preparatorily purified by salt preciptation.

为确定一条采用亲和层析技术分离纯化酵母中磷酸激酶的较佳工艺路线，采用分步盐析初步纯化己糖激酶，考察硫酸铵饱争度对盐析效果的影响；以ATP为配基，SESA活化纸纤堆素制备亲和层析剂，确定最佳偶联条件和最佳静态吸附pH值后，将粗酶液直接上亲和层析柱，看分离纯化的效果；在载体和配基之问接上1。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。