查询词典 affinity constant

Based on the physical meaning of each term in the linear parameter log I (a constant correlating the affinity of solut e t o stationary phase) of stoichiometric displacement model for retention o f solute, the thermodynamic characteristics of log I of solute in revers ed-phase liquid chromatography were investigated theoretically. This poi nt was tested by experimental data with two linear relationships:(1) plot of log I vs 1/T with apolar, polar small solut es and proteins;(2) plot of log I vs log Po/w (partition co effi cient of solute between n-octanol and water) with apolar and polar smal l solutes.

依据液相色谱中溶质计量置换保留模型及线性参数log I(与1 mol溶质对固定相的亲和势大小有关的常数)，通过作图得知非极性和极性小分子溶质及生物大分子的log I与绝对温度的倒数1/T，以及小分子溶质的log I与其在正辛醇-水中分配系数的对数log Po/w呈线性关系，从两方面进一步证明了log I具有热力学平衡常数的性质。

Interaction between fusion peptide of influenza virus A and its related antisense peptides was studied. A peptide was screened out with high binding ability to fusion peptide and the dissociation constant was shown at the level of umol/L. In order to quantitatively characterizing the affinity interaction, a system consisted of quartz crystal microbalance biosensor and flow injection analysis was designed and established.

据此初步结果，研究了甲型流感病毒融合肽与其反义肽的相互作用；提出了基于反义肽的组合化学法筛选目的多肽或蛋白质的亲和配基的全新的研究思路，筛选到了与融合肽具有强相互作用的多肽，其与融合肽的解离常数达到umol/L。

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中，我们发现，除非过量，否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象；过量的La〓（La〓/CaM摩尔比＞4）将引起钙调蛋白结合功能的下降，同时明显改变金属-钙调蛋白-Mas三元复合物的构象；La〓对Ca〓CaM-Mas的N末端表现高度选择性，显示了在三元复合物中两种离子间的协同效应；在三元体系中，La〓与钙调蛋白的亲和力明显上升，而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异，显示了作用的选择性；Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序，La〓很可能优先与C末端结合，从而使Mas/Mas X首先与C末端结合，该顺序与钙离子相同；La〓的参与使三元复合物在动力学上更加稳定。

Based on the retention and Z (the number of solvent molecules involving in t he displacement from stationary-phase surface ), log I (a constant relating t o the affinity of 1 mol of solute to the bonded stationary phase ), and j (a const ant r elating to the affinity of 1 mol of solvent to the bonded stationary phase ), th e elution strength of acetic acid-water mobile phase was found to be stronger th a n that of methanol-water-TFA.

用参数Z （1 mol溶剂化溶质被溶剂化固定相吸附时从两者接触表面释放出置换剂的摩尔总数），log I （与1 mol溶质对固定相亲和势有关的常数）和j（与1 mol溶剂对固定相亲和势有关的常数）对9种蛋白质在此流动相体系中的保留进行了表征。

For the high affinity sites, binding constant decreases with the decrease of the alkyl chain lengh in the surfactants, but for the low affinity sites, binding constant increases and are not distinct when the alkyl chain is dedocyl. The binding sites are related to the alkyl chain lengh, not for the number of hydrophile groups and temperature.Isothermal titration calorimetry has been used to observe the enzyme-catalyzed hydrolysis reaction of urea.

对于高结合位点，结合常数随疏水碳链长度的减小而减小；对于低结合位点，结合常数随疏水碳链长度的减小而增大，相应的当碳链减小至C12时，低结合位点不明显，结合位点数可能只与碳链长度有关，随亲水头基和温度变化不明显。

In the absence of coorperative effect, the reaction between lectin molecules and their receptors on cell surface can be described as below when equilibrium is reached:In which LT denotes total lectin concentration, LF free lectin concentration, mva-lency of lectin, ni number of receptors with affinity constant Ki to lectin, CT total lectin concentration.

本文提出一个描述细胞与多价凝集素反应的平衡关系式和用紫外分光光度法测定游离凝集素浓度求受体数量及其与凝集素的亲合常数的方便方法。

The relative cross-reactivity of PA with AFB-1,ZON,T-2,FB were less than 0.01%and with an affinity constant for PA of about 1.54×10~8 liters per mol.It offered a foundation for developing immunoassay method for PA.

单克隆抗体与黄曲霉毒素B1、玉米赤霉烯酮、T-2毒素和伏马毒素B1均无交叉反应率，单抗的亲和常数为1.54x10~8 L/mol，为进一步建立青霉酸的免疫学快速检测方法提供了基础。3。

Competitive indirect enzyme-linked immunosorbent assay was employed to perform the affinity measurement of dissociation constant and affinity constants for bovine milk lactoferrin and IgY specific against LF using antisera from rabbit and hen as references.

以竞争性ELISA法来测定牛乳乳铁蛋白抗原与蛋黄中抗LF特异性抗体间之解离常数与亲合常数，而同时以兔与鸡之抗血清为对照组。

The affinity constant of the anti-SQ polyclonal antibody 1405~# was 1.22×10~8L/mol. The cross reactivity with other SAs was less than 0.17%. Three hybridoms cell lines secreting monoclonal antibody against SQ were obtained.

同时，以SQ-BSA为免疫原免疫Balb／c小鼠，经融合实验获得了三株分泌抗磺胺喹噁啉单克隆抗体的杂交瘤细胞株SQl_(12)A_(10)，SQ_(26)B_7，SQ_(28)H_7。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷