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The citric acid-producing strain Aspergillus niger 2363-2, was isolated and used in our research. The fermentation conditions were optimized as follows: sucrose as carbon source 8. 0-14. 0%, ammonium chloride as nitrogen source 3. 0-4. 0g/l, initial pH 2. 0, and cultural temperature 30℃. The influential factors of formation of spores were also studied, and a series of measures were taken to restrain the spore formation on the basis of our experimental results. With the consideration of the cultural environment and microbial cell itself, the factors affecting the duration of citric acid-producing activity of microbial cells were examined, and it is found that undissociated citric acid and the ageing of microbial cells are the main factors. The experimental results also show that partial replacement of fermentation broth can lengthen the duration of citric acid -producing acitivity of microbial cells.

筛选出了用于试验研究的柠檬酸发酵用菌Aspergillus niger 2363-2,确定了其最适发酵条件—碳源选用蔗糖,浓度8.0~14.0%,氮源为NH〓Cl,浓度3.0~4.0g/l,发酵初始pH值为2.0,培养温度30℃;讨论了分生孢子产生的影响因素,并通过试验提出了抑制分生孢子产生的措施;从分析微生物所处的环境和微生物本身两个方面出发,探讨了影响微生物菌体产酸活性持久性的因素,认为分子态柠檬酸和菌体本身的老化是影响微生物产酸活性持久性的两个主要因素,并提出采用部分置换发酵液发酵能延长微生物菌体的产酸活性。

With the analysis of the data and policies from Hengyang government, this paper has investigation into the mechanisms of uneconomic factors affecting the house prices, and in order to promote the development of real estate in Hengyang city, some valuable suggestions and countermeasures are given based on the research..

本文以衡阳市商品房价与政府房地产政策为基础进行分析,对房价上涨的非经济因素的作用机理进行研究,并在此基础上对进一步控制商品房价格合理上涨提出具有针对性的建议与对策,以促进衡阳市房地产事业的健康发展。

They provide the ungoverned spaces from which terrorism, criminality, drugs and migration can spill over borders affecting nations around the world.

它们会造成无政府状态,恐怖主义、犯罪、贩毒和非法移民会从这里滋生并蔓延,从而影响到世界各国。

The results show that heating temperature and holding time are two important factors affecting the grain size and uniformity.

实验表明,加热温度和保温时间是影响晶粒尺寸和均匀性两个重要的因素。

The main factor affecting the frequency characteristic were given as the following:the coefficient of corrosion of the Al foil surfaces the figure of the hole of scorrosion,and the character of the oxide film on the surface of the anode and cathode;the fabric of the unimpregnated liner in the capacitor,as well as the resistance and steeping of the working electrolyte and the construction of the capacitor.

影响频率特性的主要因素有:阴极和阳极铝箔表面腐蚀系数的大小及腐蚀坑洞的形状;阴极和阳极铝箔表面氧化膜的性质;电容器中衬垫纸的构造;工作电解液的电阻和浸渍情况以及电容器的内外结构尺寸等。

The electrode structure of the uniplanar scattering-field capacitive sensor is the essential factor affecting sensor performance.

该文在电磁场分析的基础上,采用有限元分析的方法研究了传感器电极结构与敏感特性之间的关系,并通过实验对分析结果进行了验证。

Jagged1 increases the number of bipotent colony-forming unit-granulocyte macrophage and unipotent progenitors (CFU-granulocytes and CFU-macrophages), without quantitatiely affecting terminal cell differentiation, whereas Delta1 reduces the number of CFU-GM and differentiated monocytic cells.

Jagged1增加双潜能细胞和单潜能祖细胞(CFU-G和CFU-M)的数量,而并不影响终末分化细胞的数量;另一方面,Delta1减少CFU-GM和分化的单核细胞数量。

The lifetime of the electrical submersible pumping unit is the main factor affecting the overhaul period of electrical pumping unit.

对电泵井故障模式的调查和统计表明,潜油电泵机组使用寿命是影响电泵井检修周期的主要因素。

The main factors of affecting the stability of submarine pipeline are analyzed, and the result shows: effect of Wake has disadvantage for pipeline stability, weight of per length unit pipeline is a important factor affect the stability of pipeline.

根据所建模型对影响裸置管线横向稳定性的各项因素分析表明,忽略尾流的影响会严重高估裸置管线的在位稳定性,影响管线稳定性最主要的因素是单位长度管线重量。

The aim of this research is to find out the experimental parameters that affecting the results when analyzing bacteria by MALDI TOF MS, and to set up a general sample preparation protocol for establishing mass database of Bacillus anthracis and Yersinia pestis. It will play an important role in rapid identification of bacteria, and tracing its origin in the scenario of bioterrorism and outbreak of infectious disease for microbial forensic analysis. Methods:As for the universalness of sample preparation protocol for bacterial analysis by MALDI TOF MS, bacteria being investigated included: gram-positive bacteria including B.

本研究通过对MALDI TOF MS分析细菌的方法进行系统的研究,阐明影响MALDI分析的重要实验因素的作用,并建立MALDI TOF MS分析细菌的标准实验方法,从而实现不同菌种和同一种内不同菌株的鉴定,为建立炭疽芽孢杆菌和鼠疫耶尔森氏菌的质量图谱数据库奠定基础,从而能够快速准确地检测和鉴定我国不同来源的炭疽芽孢杆菌和鼠疫耶尔森氏菌,在生物恐怖或传染性疾病爆发时,作为微生物法医学的一种重要技术,快速、准确地鉴定细菌,并追踪其来源。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

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