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adriamycin相关的网络例句

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Adriamycin , sodium salicylate and acyclovir were used as model drugs to investigate the encapsulation efficiency of alginate-chitosan microcapsules to drugs containing different kinds of charge.

以阿霉素、水杨酸钠与阿昔洛韦为模型药,考察海藻酸钠-壳聚糖微囊对电性不同的药物的包封率。

MethodsThe tumor xenografts model was established by injecting the mouse lymphocytic leukemia cells (P388) in the subcutis of anterior axillary of Kunming mice, and then was treated with CZBG by intragastric administration and different doses of adriamycin by intraperitoneal injection.

方法将小鼠淋巴细胞白血病细胞系P388细胞接种于昆明小鼠腋前皮下构建小鼠移植瘤模型,予复方浙贝颗粒联合不同剂量的阿霉素治疗。

Objective: To investigate the role of superoxide dismutase in Adriamycin-induced heart failure and the protective effects of Sini decoction.

中文摘要:目的:探讨超氧化物歧化酶在阿霉素诱导的心力衰竭中的作用以及四逆汤的可能的保护机制。

Objective To explore the protective effect of taurine on toxic cardiomyocytes induced by adriamycin and its mechanism.

目的 应用阿霉素建立心肌细胞中毒模型,观察牛磺酸对中毒心肌细胞的保护作用并探讨其机制。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体;2)利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达;3)构建ZNRD1的小干扰RNA载体,并测序鉴定;4)利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞(AGS、SGC7901、MKN28)和小鼠成纤维细胞(NIH3T3),G418筛选后进行鉴定;5)利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞(SGC7901)、正常胃组织上皮细胞(GES-1)、对长春新碱耐药的胃癌细胞(SGC7901/VCR)、药敏白血病细胞(HL-60)、对长春新碱耐药的白血病细胞(HL-60/VCR),G418筛选后进行鉴定;6)利用MTT实验检测ZNRD1高/低表达对细胞(AGS、SGC7901、MKN28、NIH3T3、GES-1)生长的影响;7)通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响;8)通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响;9)通过流式细胞仪分析ZNRD1高/低表达对细胞(AGS、MKN28、NIH3T3、GES-1)的细胞周期的影响;10)通过流式细胞仪分析上调ZNRD1对细胞(AGS、MKN28、NIH3T3)的凋亡的影响;11)通过MTT实验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)体外药物敏感性的影响;12)通过肾包膜下移植法检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR)体内药物敏感性的影响;13)通过流式细胞仪分析ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)内阿霉素蓄积和泵出的影响;14)通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响;15)通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60)凋亡敏感性的影响;16)通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响;17)利用RT-PCR、Western blot对基因芯片的结果进行鉴定;18)利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用;19)利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用;20)利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响;21)利用反义核酸技术抑制p21的表达;通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响;22)利用反义核酸技术抑制p27的表达;通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响;23)利用脂质体转染法上调Skp2的表达;通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响;24)利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响;25)利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响;26)利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响;27)利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞(SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR)多药耐药表型的影响;利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

the objective of this study was to investigate the protection by apigenin against adriamycin-induced injury and its underlying mechanisms in normal blood cells.

小 摘要:本研究探讨芹黄素(apigenin,api)对化疗药阿霉素损伤正常血细胞的保护作用及其机制。

But injury of normal tissue is one of important factors which have to stop killing tumor cell thouoghly, in all process of tunor therapy Since the injury all over argans and cells of the body by free radical, especially significant side-effect to heart via ariblastine ,is universally accepted, we chose Adriamycin as chemical therapy medicine in our study.

由于阿霉素具有比较公认的自由基周身性损伤、特别是对心脏损伤的突出副作用,因此,本课题选择阿毒素作为化疗药物,模型制作采用在SD大鼠肝表面种植Walker-256瘤细胞株,于种瘤后第11天按2mg~(-1)kg体质量给单纯化疗组和化疗保护组的大鼠腹腔注射阿霉素,正常对照组和荷瘤对照组按相同比例腹腔注射生理盐水;同时,每日上午按0.5ml/100g体质量经胃给于化疗保护组抗氧化剂混合液,其余各组每日给于相同剂量的三蒸水;下午按0.1ml/100g体质量经胃给于化疗保护组维生素E溶液,其余各组每日给于相同剂量的处理过的油。

But injury of normal tissue is one of important factors which have to stop killing tumor cell thouoghly, in all process of tunor therapySince the injury all over argans and cells of the body by free radical, especially significant side-effect to heart via ariblastine ,is universally accepted, we chose Adriamycin as chemical therapy medicine in our study.

由于阿霉素具有比较公认的自由基周身性损伤、特别是对心脏损伤的突出副作用,因此,本课题选择阿毒素作为化疗药物,模型制作采用在SD大鼠肝表面种植Walker-256瘤细胞株,于种瘤后第11天按2mg~(-1)kg体质量给单纯化疗组和化疗保护组的大鼠腹腔注射阿霉素,正常对照组和荷瘤对照组按相同比例腹腔注射生理盐水;同时,每日上午按0.5ml/100g体质量经胃给于化疗保护组抗氧化剂混合液,其余各组每日给于相同剂量的三蒸水;下午按0.1ml/100g体质量经胃给于化疗保护组维生素E溶液,其余各组每日给于相同剂量的处理过的油。

Results Hela/MMC cells possessed the ability of 5.02 fold resistance to MMC and 2.03 fold cross-resistance to Capsulation and maintain the sensitivity to vincristine, adriamycin and 5-flurouracil (5-Fu).

结果 Hela/MMC细胞对MMC有5.02倍耐药,对DDP有2.03倍交叉耐药,对VCR、ADM和5-Fu保持敏感;耐药细胞核分裂指数增加;耐药细胞内丝裂霉素蓄积量比亲代细胞低32.64%(P.01)。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株(KB-A-1)的多药抗药性问题,本文从反义核酸和反基因核酸角度,通过MTT法检测细胞生长情况,ELISA法检测基因表达产物P-gp表达水平的变化,对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

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