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MSCs from human BM were isolated by Ficoll density centrifugation cultured and expanded in DMEMLG containing FBS. MSCs are adherent and fibroblastic, and maintained similar morphology as passaging.

从人骨髓中分离和培养间充质干细胞，在原代和传代培养中其形态学上均为贴壁、纤维状的细胞，随著代次的增加细胞形态更加趋於一致。

The adherent cells were mounted on a cover slip and stained with Hoechst 33342,and subsequently stained with mouse anti-human ant...

结果荧光显微镜下可见BGC-823细胞系中存在SP细胞，且该类细胞体积较小，约占细胞总数的1.8%；免疫荧光染色可见爬片细胞中ABCG2+细胞即为SP表型细胞。

METHODS: Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells. DMEM medium containing fetal bovine serum, penicillin, streptomycin and L-glutamine was added. Following several adherences and purification, the floating cells were discarded. Thus, many adherent cells with a confluence were collected. When cells were 60%-80% confluent, cells were digested by trypsin for subculture.

无菌条件下，应用Percoll密度梯度离心法分离脐血标本，收获中间层细胞，加入含胎牛血清、青霉素和链霉素、L-谷氨酰胺的DMEM基础培养液，再经反复贴壁纯化，除去悬浮生长细胞，得到较多呈融合状态的贴壁细胞，待细胞达60%~80%融合时胰酶消化传代。

Assessment of Gastric Sensitivity, Compliance and Accommodation: The stomachs were distended isobarically by barostat via a double-lumen polyvinyl tube with an adherent plastic bag, gastric sensitivity was measured by perception threshold and gastric compliance was calculated as the slop of the pressure-volume curve.

胃敏感性、顺应性和容受性的检测：用barostat及双腔气囊导管对胃进行等压扩张，以患者的不适阈代表胃对扩张的敏感性，以压力-容量曲线的斜率反映胃顺应性；将气囊压力恒定为MDP+2mmHg，以进食前后胃内平均容量的差值来反映近端胃的容受性。7。

Sobrinus to synthesize insoluble polysaccharide was enhanced in the adherent phase, which may clarify why bacteria in biofilms are highly cariogenic.

培养时间和蔗糖浓度增加，导致远缘链球菌合成的水不溶性胞外多糖较游离状态明显增加；生物膜特殊的生长环境，是造成这种差异的可能原因。

With their special adherent roots known as 'crampons', however, they can anchor themselves so strongly, even to very slippery surfaces, as to be almost impossible to remove undamaged.

其特殊附着的根称为' crampons '，不过，他们可以锚自己如此强烈，甚至很滑表面，以几乎是不可能的，以消除完好无损。

Cupules solitary; bracts imbricate, scalelike, linear, or conical, adherent, prostrate, or reflexed.

壳斗孤独；覆瓦状，鳞片状，线形的苞片，圆锥状，附着的或，匍匐，或反折。

Blood leucocyte phagocytosis to Staphylococci aureus and phagocytic activity of adherent head kidney cells were unaffected by dietary vitamin C.

鱼体非特异性的细胞免疫活动(包括血液白细胞和头肾细胞的吞噬活动)也不受维生素C的影响。

The Mason is called a "true Noahida", ie an adherent of the pre-Christian and pre-Mosaic system of undivided mankind.

梅森被称为&真正的Noahida &，即一个贴壁前的基督教和前花叶系统的不可分割的人类。

Results LPS exposure, which had no influence on mean arterial pressure, induced a significant increase in the number of rolling and adherent leukocytes and mast cell degranulation, a progressive increase in albumin efflux, and a decrease in the velocity of red blood cells in venules.

结果 LPS在不影响血压的情况下，显著增加了细静脉内白细胞滚动和黏附数目、血浆白蛋白漏出和肥大细胞脱颗粒率，降低了细静脉红细胞流速。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。