查询词典 adenovirus

The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

Objective To investigate the effect of adenovirus-mediated gene therapy with vascular endothelial growth factorand basic fibroblast growth factordelivered into the subdermal space to treat compromised skin flaps in rats.

目的探讨以腺病毒载体局部应用血管内皮生长因子和碱性成纤维细胞生长因子基因治疗对大鼠缺血皮瓣生存的影响。

ABSTRACT: Objective In order to investigate the treatment of bone defect repairing by recombinant adenovirus transudation the hBMP7 gene, the vector of hPMP7 recombinant Ad was constructed.

目的 为了研究人骨形态发生蛋白7(hBMP7)导入骨缺损模型中的治疗作用，构建重组腺病毒载体。

Moreover, overpression of Wnt10b in the organ culture of vibrissae HF has been manipulated by infected the recombinant adenovirus expressed Wnt10b protein(Adwnt10b).

Ki67在毛母质中的表达变化规律以及在毛母质中的表达部位与Wnt10b具有一致性。2、我们对Wnt10b重组腺病毒进行了逐级扩增并应用氯化铯超高速密度梯度离心法纯化，病毒最终滴度达到109 PFU/ml；3、首次发现加入Wnt10b重组腺病毒可明显促进体外器官培养的触须毛囊毛干和内根鞘生长，这与本课题组在在体动物实验中发现的现象一致。

The recombinant adenovirus was then large amplified and purified with Viraprep purification column and desalted.

大规模扩增重组腺病毒并用Vira-prep纯化柱纯化。

Objective To improve the construction of adenovirus expression vector harbouring widetype tumor suppressor gene PTEN.

目的 为改进构建野生型抑癌基因PTEN重组腺病毒表达载体的方法。

In this study, we immunized Balb/c mice with DNA and recombinant adenovirus-based SARS vaccines.

本论文的主要研究方向是以Balb/c小鼠的动物模式，测试带有SARS病毒基因的DNA与重组腺病毒疫苗。

Methods Bladder cancer cell line 253J and human fibroblast cell line MRC-5 were transfected by the recombinant adenovirus of different multiplicities of infection,and the infection rate was measured by observing the expression of enhanced ...

利用不同感染复数的重组腺病毒携带增强型绿色荧光蛋白基因感染膀胱癌细胞253J和人成纤维细胞MRC 5，荧光显微镜下观察其感染效率。

Results UCMSCs could be effectively infected by adenovirus containing GFP gene in vitro, the transfection efficiency has the dose-effect relationship with multiplicities of infection.

结果 腺病毒介导的GFP基因对于UCMSCs具有较高的转染效率，转染效率与MOI值具有量效关系，当MOI为100倍时，转染效率达95%。

The second part of this study: the constrction of the recombinant adenovirus.

论文的第二部分：含内皮抑素基因的重组腺病毒的构建。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。