查询词典 adenovirus

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

In order to analyze the specificity of multiple PCR, multiple PCR detection system was used to detect Blastomyces albicans, Chlamydiae trachomatis, Escherichia coli, hemophilies influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, Human cytomegalovirus, duovirus, adenovirus and Henrietta Lacks strain of cancer cells.

用此多重PCR体系分别检测分离的白色念珠菌、沙眼衣原体、大肠埃希氏杆菌、流感嗜血杆菌、肺炎克雷伯杆菌、金黄色葡萄球菌、肺炎链球菌、人巨细胞病毒、轮状病毒、腺病毒和Hela细胞，分析此多重PCR体系的特异性。

These results suggest that the chimeric oncolytic adenovirus H101-F35 vector might be a useful candidate for gene therapy of cancer.

这些结果表明嵌合型溶瘤腺病毒载体H101-F35在肿瘤基因治疗中将有很好的应用前景。

Methods RNAi combinant adenovirus vector which targeted 1566 site of NF-κB p65 mRNA was constructed and the effect of p65 gene knockdown in HUVEC was detected by Western blot analysis.

构建针对NF-κBp65 mRNA序列1566位点的重组RNAi腺病毒表达载体，并利用RNAi腺病毒抑制HUVEC p65表达。

It is known that adenovirus E1A inactivates Rb normal function by directly binding to Rb. Thus, we reason if E1A blocks the normal function of Rb, it may lead to cisplatin-induced deamidation of Bcl-xL and sensitize the p53-null ovarian cancer cell, SKOV3-ip1 cells, to cisplatin.

过去已知腺病毒E1A会藉由与Rb结合而抑制它的正常功能；因此我们推论若E1A抑制Rb的正常功能之下，cisplatin的处理应该可以导致Bcl-xL 的去胺基化并且使没有p53的卵巢癌细胞SKOV3-ip1对cisplatin较敏感。

LX2 cells were transfected at the adenovirus tite pfu100, and were harvested at different times (24,48,72h), then western blotting was executed to detected the expression of ectogenous ampk.

对凋亡信号通路的进一步研究发现，Bcl-2在LX2中是低表达的，而Bax在AMPK过表达之后，表达增加，而用RNAi方法敲低Bax之后，细胞再用携带AMPK基因的腺病毒感染，LX2细胞不再发生凋亡，说明Bax参与了AMPK诱导LX2凋亡的信号通路。

Objective To construct a hTERT and Cox-2 promoter regulated oncolytic virus by replacing the endogenous adenovirus Ela and Elb promoter,respectively.

目的 应用同源重组的方法构建以hTERT和Cox-2启动子调控增殖的肿瘤特异性增殖腺病毒。

Methods The promoter of hTERT and Cox-2 was subcloned from human genomic DNA, then ligated to shuttle vector pd306 which contains the Ela and Elb gene of adenovirus to control the expression of Ela and Elb,respectively.

将hTERT和Cox-2启动子从人类白细胞基因组中亚克隆出来，并将启动子分别插入到腺病毒穿梭载体pd306上的E1A和E1B基因前，使hTERT和Cox-2启动子分别调控腺病毒必须基因E1A和E1B的表达，再将构建后的pd306和腺病毒的骨架质粒BHGE3在Ad293细胞内进行同源重组，并用重组后的病毒感染Hela细胞检测病毒对肿瘤细胞的杀伤力。

In this dissertation, we exploringly studied the new antigen epitopes and adenovirus vector vaccine for prevention and therapy of the H.

本论文首先利用基因重组技术，将Hp抗原Ure B、Hsp A、Vac A和Cag A的基因分别克隆至真核表达载体，并成功将ute B和hsp A基因融合。

Adenovirus carrying BMP-2 gene, after being mixed with fibrinous gel, was siphoned off on biodegradable scaffolds.

将BMP-2腺病毒载体(Ad-BMP-2)与纤维蛋白凝胶混合后复合PLA／PCL支架，移植修复骨缺损。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。