查询词典 adenovirus

The adenovirus were transinfected to the MSCs, which were then combined with algin to form calcium alginate gel compound.

腺病毒转染后与藻酸钠复合形成藻酸钙凝珠。建立大鼠股骨干中段连同骨膜截去6 mm的缺损模型。F344近交系大鼠48只按随机数字表法分为6组，每组8只。

Objective To explore the influence of E1A gene of adenovirus type 5 on the sensitivity of breast cancer cells to chemotherapeutics.

目的 探讨E1A基因对乳腺癌细胞化疗药敏感性的影响。

To construct recombinant adenovirus,the N and G genes were respectively cleaved from the pMD18-T plasmids by double restriction endonuclease,and then cloned into shuttle vector pAdTrack-CMV coinstantaneously.

经卡那霉素抗性和酶切筛选，最终成功构建了含有N基因和G基因的重组穿梭载体pAdTrack-CMV-N-G。

Objective: To evaluate the selectively oncolytic effect of conditionally replicating adenovirus CNHK500 in breast cancer.

目的：观察选择性增殖腺病毒CNHK500对乳腺癌的特异性杀伤作用。

Methods: We used virus proliferation assay cell viability assay to evaluate the proliferation and cytolysis selectivity of CNHK500. And we used Western-blot to confirm the expression of adenovirus CNHK500 E1A and E1B in cancer and normal cells.

行病毒增殖实验和细胞生长抑制实验，验证CNHK500选择性复制和杀伤能力；Western blot检测腺病毒E1A和E1B在细胞中的表达。

In contrast, cancer cells lacking functional P53 would be expected to be sensitive to viral replication and subsequent cytolysis. However P53 defections wildly exist in most of human cancer cell, deletion of E1B-55kD gene can make the adenovirus target at p53 mutation tumor cell.

以往的研究表明，大部分人类肿瘤中均存在p53基因突变，因此E1B55kD蛋白缺失的重组病毒只能在P53功能异常的肿瘤细胞中复制，而在正常细胞中该重组病毒由于缺乏对抗P53蛋白的病毒产物，不能有效复制，因此具有肿瘤靶向治疗的特点。

Antitumor efficacy of replication-competent adenovirus CNHK500 in vivo was associated with increased dosage of CNHK500. Conclusions: The results prove that CNHK500 has highly proliferation selectivity and potent cytolysis effect on breast cancer.

肿瘤选择性增殖腺病毒CNHK500可选择性在端粒酶阳性的乳腺癌细胞中复制，并产生体内外杀伤作用。

To explore the possibility of the targeting expression of Escherichia coli cytosine deaminase gene by adenovirus-mediated transfer in human pancreatic carcinoma cells.

探讨腺病毒介导的大肠杆菌胞嘧啶脱氨酶基因在胰腺癌细胞中靶向性表达的可能性及其作用。

Objective: To prepare an adenovirus, Ad-CD, expressing bacterial cytosine deaminase,and use it for tumor suicide gene therapy.

目的：制备含胞嘧啶转氨酶基因的重组腺病毒并进行体外肿瘤的自杀基因治疗。

Objective: In order to investigate whether the recombinant adeno-associated virus -mediated foreign genes, LacZ/hVEGF, could pass the blood brain barrier by intra-carotid artery delivery or not and to investigate whether the foreign genes could be expressed in vivo in ischemic brain of the focal embolic stroke rats or not, a high titer and completely adenovirus free recombinant adeno-associated virus vector was constructed and studied.

目的：为了检测腺相关病毒载体携带的人类血管内皮因子基因能否通过血脑屏障及能否在脑组织中表达其基因产物，我们构建了一种新型、高滴度、无腺病毒污染的rAAV携带的报告基因和hVEGF基因分别经颈动脉对自体血栓局部脑卒中大鼠进行了研究。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。