查询词典 adenovirus

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

However, molecular conjugate vector containing adenovirus could enter cells via adenovirus receptor which exists on large range type of cell membrane, its cell targeting is affected.

然而，由于在不同组织细胞膜存在腺病毒受体，分子偶联载体可能通过腺病毒受体进入细胞内，致使基因转移靶向性受到影响。

This study was to evaluate enhancive effect of trichostatin A, a histone deacetylase inhibitor, on the transfection efficiency of adenovirus in ovarian carcinoma cell line A2780, and explore its possible application to adenovirus-based gene therapy.

本研究通过观察组氨酸去乙酰化酶(histone deacetylase， HDAC)抑制剂Trichostatin A对卵巢癌细胞株A2780 CAR表达水平的影响，探讨HDAC抑制剂在腺病毒载体基因治疗中应用的可能性。

The study mentioned above indicated that in addition to anti-adenovirus effect, prior effect of the contained- Qingfei oral liquid serum was to adjust the function of the body and histiocyte. So it could treat the child with adenovirus pneumonia. This embodied the multitarget effect Chinese herbs on treating viral diseases.

以上研究表明，清肺口服液含药血清除了有抗腺病毒的作用外，更重要的在于调节机体和相应的组织细胞功能，从而达到治疗小儿腺病毒肺炎的目的，体现了中药治疗病毒性疾病作用的多靶点效应。

Recombinant adenovirus containing major outer membrane protein gene of Chlamydia psittaci of chicken origin were successfully constructed with human adenovirus type 5 vector which lack El and E3 genes.

利用E1和E3缺失的人腺病毒5型载体，首次在国内外成功构建了含有鸡源鹦鹉热衣原体主要外膜蛋白基因的重组腺病毒。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

Considering undividing cells might be transfected with adenovirus vector andmacrophages could be activated by IL-2,we transfected mIL-2 gene into the freshlyisolated peritoneal macrophages with recombinant adenovirus,and pulsed with tumorantigen in vitro,in order to enhance the immune effector function as well as theantigen presentation function simutaneously,and acquired more effective actions ofantitumor.

本研究选用可转染非分裂细胞的缺陷型腺病毒为载体，将活化巨噬细胞的小鼠IL-2基因转染至小鼠腹腔巨噬细胞，并在体外行肿瘤抗原冲击，以期在增强其免疫效应功能的同时充分发挥其抗原提呈功能，从而取得更好的抗瘤效果。

Results: NIH3T3 cells were effectively transfected by adenovirus containing VEGF gene in vitro, the transfection efficiency was in a dose-effect manner with multiplicities of infection of the adenovirus.

结果：携带hVEGF基因的重组腺病毒对于NIH3T3细胞具有较高的转染效率，转染效率与病毒感染复制数(multiplicities of infection， MOI)具有量效关系。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

The replicative recombinant adenovirus is more effective than the replication defective adenovirus used generally at present in replicative ability, oncolytic ability and the expression of exogenous gene in the tumor cell.

构建的复制型重组腺病毒在肿瘤细胞内的复制能力、溶瘤特性和外源基因的表达明显优于目前广泛应用的复制缺陷型重组腺病毒。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。