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The invention discloses a method for extracting polysaccharide from chiretta and an application, which comprises breaking dry chiretta via a breaker into powder, arranging into an extraction pot, adding ligarine to reflux and defat, adding distilled water into defatted residues to extract, processing alcohol precipitation and centrifugation to obtain deposits, removing protein, processing acid hydrolysis and gel chromatography separation, freezing and drying to obtain active chiretta polysaccharide.
本发明公开了一种从当归中提取多糖的方法及应用。干燥当归用粉碎机粉成粉末,置入提取罐中,加入石油醚回流脱脂,向脱脂后的残渣中加入蒸馏水提取,醇沉、离心得沉淀,除去蛋白、酸解、凝胶色谱分离、冷冻干燥得到活性当归多糖。
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The results show that, by the way of optimizing preparation condition, the spherical zirconia powders with suitable particle size distribution are prepared using chemical precipitation method. Using heterogeneous chemical precipitation coating technique, the sph, erical nickel coated zirconia powders are prepared and the heterogeneous nucleation can be done on the surface of spherical zirconia powders. The thickness of coated layer, homogeneous degree and crack of surface can be controlled by the way of controlling powder concentration, adding rate of reactants feeding and adding surface active agent.
研究表明,采用化学沉淀法,通过优化制备工艺条件,可以制备粒径分布较均匀的球形氧化锆粉体;利用非均相化学沉淀包裹技术制备的金属镍包裹氧化锆微球粉体,球形氧化锆颗粒表面作为异相成核的场所,通过控制颗粒浓度、加料速度以及添加表面活性剂等工艺条件,可以调控包裹层厚度、表面均匀度以及表面裂纹等。
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After finish the digestion, then flow back to the blood.also, during the period of liver and gallbladder concertrate in the hepatoenteral circulation,the bile acid can drain massively with the excrement.and more seriously after adding the fat feed stuff.if this condition go on, the fat cannot be absorbed in time and the energy will be wasted and cause some disease and etc.but after adding the appropriate exogenetic emulsifier,the question can be solved.
作为正常的机体,胆汁酸由肝脏分泌,进入肠道,在完成消化,回流入血液,又在肝脏和胆囊重新富集的肝肠循环的过程中,会经过粪便大量流失。在脂肪含量较高的饲料配方下,流失更为严重。日肝脏分泌量不足弥补流失量,会造成脂肪消化的阻滞,能量的浪费,且易诱发肝胆代谢紊乱等各种疾病。得不到消化吸收的油脂,在肠道内被噬油微生物降解,使噬油微生物类群大量增殖,打破原来肠道内微生态平衡,产生腹泻等菌群失调症,以上的问题,都是由于脂肪乳化作用不足和脂肪代谢失调造成的。在补充了合适本物种须求的外源性乳化剂后,问题可得到解决。
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The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.
向血管平滑肌组织块中,加入用DMEM配制的0.15%胶原酶II溶液,水浴箱中作用1.5h,至组织呈絮状,用0.02%的EDTA清洗,然后,加入0.125%胰蛋白酶溶液,于37℃水浴振荡消化,即可得到分散的平滑肌细胞,用10%新生牛血清的培养液终止酶的作用。加入含20%新生牛血清的培养液,调节细胞密度为5×104~5×105个/L,接种,放入5%CO2、37℃培养箱中培养。当细胞达到70%~80%融合时即可按常规方法传代。
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Inhibitors including sodium dodecyl sulfate, boric acid, sulfur, and potassium fluoroborate (KBF4) are added to compare the surface quality of AZ91 casting Experimental results show that adding sulfur in green sand obtained AZ91sand castings with satisfied surface quality and adding KBF4 in green sand produced sand castings with best mechanical properties.
实验结果显示添加硫磺,可得到较佳模砂性质且有效控制产生较好的金属表面品质,添加氟硼酸钾有较佳机械性质。
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One test is circulating test which tailing water is filtered and reused,another test is adding a little frother in water before adding coal sample.
结果表明,循环水中积聚的有机物主要是醇、酮、羧酸盐等含氧化合物;有机物积聚可提高精煤回收率,改变浮选的选择性
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From the results, it was observed that the transfer migrate coefficient for hendecane was larger than that for kerosene; the transfer mass resistance increased and decreased with adding Span 80 and TBP respectively; there was little effect by adding liquid paraffin.
发现苯胺在十一烷/水界面的传质系数比在煤油/水界面上的大;在油相中加入span80和磷酸三丁酯会使传质阻力分别增加和减少,而液体石蜡的加入几乎不影响传质速度。
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The results show that after adding RBW 0.5-2.5% bentonite, the highest removal of COD and Kjeldahl Nitrogen was 32% and 24%; after adding RWB 1~5% lime, the highest removal of COD and Kjeldahl Nitrogen was 34% and 40%.
结果表明按土水比添加0.5%~2.5%的膨润土处理后,废水中的COD和凯氏氮的最高去除率分别为32%和24%;添加1%~5%的生石灰处理后,废水中的COD和凯氏氮的最高去除率分别为34%和40%。
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Adding fresh medium to cultures, when they had been cultured for 5-8 days, could promote cell sustained division, after which, adding a medium with lower concentration osmatic substance or without osmatic one could futher stimulate the cell division and ensure the active growth of the cell masses.
采用液体浅层培养方法,对埃斯基和雷蒙特红豆草的原生质体进行了培养。埃斯基花茎愈伤组织的原生质体在VK和Kao's培养基中2-6天分裂,2周内形成细胞团,19天时的分裂率和克隆率分别为71.41-76.19%、64.78-67.03%。
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Using slow-adding and fast-agitating method, protective coating is prepared by adding proper filler, plasticizer, curing agent, solvent and so on, then the formula is optimized according to analysis of the performance of self-production and other production made at home and abroad, including appearance, viscosity, solid content, corrosion resistance, etching ratio, compactibility, bonding force and peelable ability.
在基料中配以合适的填充剂、增塑剂、固化剂和溶剂等,采用慢加快搅法制备此种涂料,并通过对比自制涂料和国外化铣保护涂料的性能及分析数据,即外观、黏度、固体含量、抗蚀性、浸蚀比和致密性,优化保护涂料的配方。
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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.
在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。
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In fact,I've got him on the satellite mobile right now.
实际上 我们已接通卫星可视电话了
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The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.
世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。