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The changes include:(1) Put the urediniospores into the tube with two steel balls and 1. 6 g/L Chelex-100,the uredinia may be picked up with airing host tissues.(2) Add KOH into the extraction buffer instead of NaOH and vortex the tube violently for 30 min.(3) Prick a hole under the bottom instead of picking out of the steel balls, then centrifuge the tube to toss the liquid into the sheathed tube.

改进的 内容包括：(1)在有二钢珠和1.6 g／L Chelex的离心管中加入夏孢子，夏孢子可以来自带有风干寄主叶肉组织的夏孢子堆；(2)在离心管中加入KOH而不是NaOH，然后剧烈斡旋30 min；(3)将离心管管底穿一小孔，套上一新离心管，短暂离心甩出内容物。

All of these little things add up to a longer road to Cleveland, and a wearier team once they arrive.

所有这些小事加在一起不仅让通往克利夫兰的路越来越长，而且让我们在到达的时候变得更加疲倦。

All of these little things add up to a longer road to Cleveland, and a wearier team once they arrive.

所有的这些都只是通往克利夫兰大道的垫脚石，一支令人倍感厌恶的球队正在那凝望着湖人众将士们。

"Little things, but when you add them up together it is big things."

&改善是一点点慢慢发生的，但是到最后你获得的是一个了不起的进步。&

Although they are separate, together they all add up to something.

虽然他们是分开的，但是，一起他们全部加总为某事。

Although they are separate, together they all add up to something.

尽管它们之间是相互独立的，但同样的，它们加起来就是某样东西的总和。

Exactly how many octaves you add together is entirely up to you .

具体多少倍频你叠加在一起，这完全取决于你。

Would you like to spice up your life, add some romance, or find your soul mate?

你是否想香料你的生活，添加一些浪漫，或找到您的灵魂伴侣？

Melt the butter in a pot and add sugar, vanilla sugar, and the water, then boil up briefly.

融化牛油，加入砂糖，香草糖及水，然后稍微煮滚。

Boil up some water with ginger and spring onions. Add in straw mushrooms and cook for 4 minutes. Drain and soak in cold water.

把姜葱及适量之水煮滚，放下草菇煮4分钟，捞起用清水浸冷，取起抹干水。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"