查询词典 actual effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The effect of treatment is negatively correlated with the course of disease, generally, the effect is much worse with the course of disease is much longer; The effect is positively correlated with the course of treatment, it is better if the course of treatment is longer.The group of more than one year is much excellent than the group of less one year. The ex-electroencephalogram before treatment is normal, on edge and a little abnormal, the effect is better. As regards CT or MR result, there is difference between the effective group and the ineffective group. The result of CT and MR is negative, the effect is better, and the effect to demyelination and leukoencephalopathy is most worst.However,for the tipe of much abnormality, the effect is the most worst.The effect is related to course of disease.The effect is better if the course is shorter.There is no difference in the tipe of disease the symptoms before episode, the frequency of episode, the inducing reasons, the age, sex and so on.

病程的长短与疗效存在负相关关系，病程越长疗效越差；疗程与疗效存在正相关关系，疗程越长疗效越好；疗前脑电图的异常程度与疗效存在负相关关系，正常、边缘及轻度异常者，疗效较好，重度异常者疗效最差；无效组与有效组在CT及MR检查结果上有差异，有阴性者疗效好，阳性者疗效差，脱髓鞘及脑白质病疗效最差的趋势；疗效与性别无相关性；无效组与有效组在发作类型、既往病史、辨证分型、发作先兆、疗前发作频率、诱因、发病年龄、就诊前所用药物方面无差异。

As a whole, this article investigates the investment effect problem from a new view of regional economics. It covers the economic, social and ecological effect of regional investment; it defines the concepts of investment effect and regional investment effect, deciphers the connotation, classification and influential factors of investment effect, and establishes a basic theory framework of investment effect research; constructs a fire-new regional investment effect evaluation model that could evaluate comprehensively the economic, social and ecological effect of regional investment, including evaluation index system and evaluation method, which supplies the gap of regional investment effect evaluation in academic community.

总体上看，本文从区域经济学这一新的视角研究投资效应问题，内容涵盖了区域投资的经济效应、社会效应和生态环境效应；界定了投资效应、区域投资效应的概念，解读投资效应的内涵、分类与影响因素，建立了投资效应研究的基本理论框架；构建一个对区域投资的经济效应、社会效应和生态环境效应进行综合评价，包括评价指标体系和评价方法在内的全新的区域投资效应评价模型，弥补了学术界在区域投资效应评价方面的空白。

The chief results were summarized as follows: In the toxicities tests to aquatic animal in the room, the results showed that Deltamethrin and Lambda-cyhalothrin, which the actual toxicities in the 24 hours on fish, loaches and tadpoles all are above 100%, were the most high toxicity, and that Chlorpyrifos and Triazophos, which the actual toxicities in the 24 hours on them are bellow 54%, were the more high toxicity, and that Fipronil and Abamectin were relatively safe, which the actual toxicities in the 24 hours on them are 22%, and that Bisultap was completely safe, which the actual toxicities in the 24 hours on them are bellow 4%.

研究结果表明：溴氰菊酯、高效氯氟氰菊酯对水生动物的毒性最高，对鱼、泥鳅、蝌蚪24h急性毒性都为100％，毒死蜱、三唑磷毒性次之，对鱼、泥鳅、蝌蚪24h急性毒性均在54％之上，氟虫腈、阿维菌素较安全，对鱼、泥鳅、蝌蚪24h急性毒性均在22％之下，杀虫双对水生动物最安全，对鱼、泥鳅、蝌蚪24h急性毒性为4％以下。

The stress was the study of the new methods of calculation the harmonic voltage and the harmonic current, and a new concept of filter ratio to evaluate the actual effect of single harmonic filter was raised considering the background harmonics. As well, the calculation mode of the single harmonic filter was also given based on the concept of filter ratio; it can offer effect data to evaluate the actual filter ratio of the single harmonic filter. At the same time, a method of designing the single harmonic filter was given, too, considering the background harmonics of the power system. In this paper, both the computer analysis and the proven had been done. And the result was shown to be that the voltage aberration and the harmonic current were decreased, and that the actual filter ratio of single harmonic filter was raised. The reason that we must consider the background harmonics when designing a single harmonic filter was also expatiated in this paper.

重点研究了考虑电网背景谐波下谐波电压、电流的计算方法，提出了用滤波率来评价单调谐滤波器安装容量利用率的概念，基于滤波率，分别给出了不考虑、考虑电网背景谐波时，单调谐滤波器的计算模型，为定量分析和评价单调谐滤波器的实际补偿效果提供了依据：并以此提出了考虑电网背景谐波的单调谐滤波器计算机辅助优化设计方法，并经计算机仿真分析、论证，减少了负载母线谐波电压畸变率和注入系统的谐波电流，有效地提高了单调谐滤波器的滤波效果，阐述了单调谐滤波器的设计过程中要考虑电网背景谐波的理论和实际意义。

In the dissertation, the study on carbon fiber reinforced concrete andplain concrete has been reviewed. Then mechano-electric effect of CFRC and plainconcrete, its mechanisms, the interaction among mechano-electric effect,piezoresistivity and seebeck effect, the electrical circuit model of concrete based onmechano-electric effect, electromagnetic emission of concrete, the mathematicalmodel of mechano-electric effect are investigated. Also the converse effect ofmechano-electric effect of CFRC and plain concrete, that is, electromechanicaleffect, and the interaction between electromechanical effect and electrothermaleffect of CFRC are examined.

本文对碳纤维混凝土和素混凝土机敏性的国内外研究现状进行了全面的评述，在大量实验分析的基础上，系统地研究了碳纤维混凝土和素混凝土的力电机敏性，研究了力电效应机理、力电效应与其它效应(压阻效应、塞贝克效应等)的耦合关系、基于力电效应的混凝土电学模型、混凝土电磁发射现象、混凝土力电效应的数学模型等；论文同时对混凝土和素混凝土的电力效应、碳纤维混凝土电力效应与电热效应的耦合关系等进行了探讨；最后开展了基于力电效应的机敏混凝土结构应用研究。

In the long run, the cumulative effect of inequality on investment is negative.（2） Inequality has moderate effect on education, and the cumulative effect is positive.（3） The effect of inequality on investment overweighs its effect on education, so inequality has a strong indirect effect on growth instantaneously. The effect turns positive and then weakly negative.

研究发现：（1）收入差距在即期对投资有非常强的负面影响，之后影响变为正，再逐渐下降至微弱的负，从长期来看，收入差距对投资的累积影响始终为负；（2）收入差距对教育的影响较弱，其累积影响始终为正；（3）由于投资对于经济增长的作用超过了教育，因此收入差距对于经济增长的间接影响主要来自于投资的渠道。

In the first section, the effect of sodium houttuyfonate on the level of lysozyme secreted by macrophages was first tested by bacteriolytic method; then the effect of sodium houttuyfonate on the level of tartaric acid resistant acid phosphatase produced in macrophages was investigated by analyzing the phenol generated in the reaction that was catalyzed by the enzyme; the third, the effect of sodium houttuyfonate on the phagocytic activity of macrophages was observed by microscopy; the forth, the effect of sodium houttuyfonate on the production of complement C4 from macrophages was tested by hemolytic method; the fifth, the effect of sodium houttuyfonate on the expression of C3b receptors on the surface of macrophages was analyzed by observing the formation percentage of zymosan wreath; the sixth, the effect of sodium houttuyfonate on the respiratory burst in macrophages was tested by flow cytometry; the seventh, the effect of sodium houttuyfonate on the production of IL-1α and IL-1β from macrophages was tested by ELISA method.

在这一章中，首先，采用溶菌法考察合成鱼腥草素对巨噬细胞分泌溶菌酶水平的影响；其次，采用游离酚法研究合成鱼腥草素对巨噬细胞产生的耐酒石酸酸性磷酸酶水平的影响；第三，采用显微镜观察法考察合成鱼腥草素对巨噬细胞吞噬功能的影响；第四，采用溶血法测定合成鱼腥草素对巨噬细胞产生的补体C4水平的影响；第五，采用酵母花环法检测合成鱼腥草素对巨噬细胞表面C3b受体水平的影响；第六，采用流式细胞术法观察合成鱼腥草素对巨噬细胞呼吸爆发的影响；第七，采用ELISA法探讨合成鱼腥草素对巨噬细胞产生IL-1α及IL-1β水平的影响。

The court further held that petitioner was a "public figure" for purposes of determining respondents' liability; that respondents were protected by the First Amendment thereby requiring petitioner to prove "actual malice"; and that based on the depositions, affidavits, and pleadings there was no genuine issue of material fact on the issue of actual malice, neither respondents' failure to investigate nor unfair editing and summarizing being sufficient to establish "actual malice."

法院更进一步举行上访人决定应答者的责任为目的是"民众演算"；哪一应答者被第一个改善保护了藉此需要上访人真实的恶意；和基于沈淀，口供和辩论那里是没有在真实的恶意议题上的物质事实的真正议题，皆不应答者无法调查也不不公平的编辑和概述充份建立"真实的恶意。"

This graduation design main is to Xi Yuan high school this the campus network of the school construction carry on of programming and design, for true accurately carry on programming design, oneself special carried on Xi Yuan a high school of actual investigate, according to actual the circumstance carry on analysis and drew a school of whole programming layout plane chart, by this actual get this time design project, in the project the equipments, facilities involve choice and install the location all have in many ways consideration, but be subjected to technique knowledge etc.

本毕业设计主要是对西苑中学这所学校的校园网络建设进行的规划与设计，为真实准确的进行规划设计，本人特别进行了西苑中学的实际勘察，根据实际情况进行分析并绘制出了学校的整体规划布局平面图，藉由此实际得出了本次设计方案，方案中所涉及设备、设施选择及安装地点均有多方考虑，但受技术知识等因素限制本设计如有纰漏敬请指出改正。

We are glad to have the opportunity to introduce to you our newly -developed product.

很高兴能有此机会向贵公司介绍我们新开发的产品。

The large artificial lakes abound with birds and wildlife.

大型的人工湖有很多的雀鸟和野生动植物。