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Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

The results show the agglutinative activity, SOD activity, CAT activity and lysozyme activity in serum of Cyprians carpio increased in contrast to that of control group. The activity of fed lectin from Porphyra yezoensis group was 3.0, 0.8, 3.9 and 1.0 times, respedctively, as much as that of control group. The activity of fed lectin from Undaria Pinnatifida group was 1.0, 0.4, 3.0 and 0.7 times, respectively, as much as that of control group. The activity of infusing lectin from Undaria pinnatifida group was 3.0, 0.3 and 1.8 times, respectively, as much as that of control group. The activity of infusing lectin from Porphyra yezoensis group was 3.0 and 0.7 times, respectively, as much as that of control group.

实验结果表明，投喂和灌喂凝集素后，鲤血清中凝集素、超氧化物歧化酶、过氧化氢酶、溶菌酶活性与对照组相比都有提高，其中投喂条斑紫菜凝集素组比投喂对照组依次提高3.0倍、0.8倍、3.9倍、1.0倍；投喂裙带菜凝集素组比投喂对照组依次提高1.0倍、0.4倍、3.0倍、0.7倍，灌喂裙带菜凝集素组比灌喂对照组依次提高3.0倍、0.3倍、1.8倍；灌喂条斑紫菜凝集素组比灌喂对照组依次提高3.0倍、0.7倍。

The radical scavenging activity of Laminaria japonica pigments was studied by the DPPH2,2-Diphenyl~(-1-picrylhydrazylassay. The total pigments showed a strong DPPH radical scavenging activity of 65.819% with the concentration of 0.52mg.ml~(-1). The DPPH radical scavenging activity of fucoxanthin was 19.85% with the concentration of 0.27mg.ml~(-1). The antimicrobial activity of pigment in Laminaria japonica was studied by the method of round paper. The results showed that the pigment extracts have activity against Escherichia coli, Pseudomonas aeruginosa, Saricina flava and Saccharomyes cerevisia. The antibacterial activity of total pigments for Saccharomyes cerevisia was the strongest with an inhibition diameter of 18.9 mm; and the antibacterial activity of fucoxanthin for Saricina flava was also much significant with an inhibition diameter of 10.2 mm.

测定了它们对自由基的清除率和对四种细菌的抑菌效果，研究结果表明，总色素和褐藻黄素的浓度与自由基清除率成正比，当总色素浓度为0.52mg.ml~(-1)时，清除率达到65.819%，高于陆生植物黑米色素和紫甘薯色素；当褐藻黄素浓度为0.27mg.ml~(-1)时，自由基清除率达到19.85%，低于紫苏色素、紫甘蓝色素、黑米色素、紫甘薯色素；对大肠杆菌、绿脓杆菌、八叠球菌、金黄葡萄球菌的抑菌实验结果显示，海带总色素对金黄葡萄球菌的抑菌活性最强，抑菌圈直径为18.9mm；褐藻黄素对八叠球菌的抑菌活性相对较强，抑菌圈直径为10.2mm。

The activity of Carboxymethylcellulase rose and peaked on September 8, and then decreased; the activity of Pectinmethylesterase decreased before September 18 ( early and middle stage of maturity), and then rose to the peak value on September 28, and then decreased dramatically; the activity of Polygalacturonasewas lower before September 18, while its change was same as activity of PE after September 18; cellulose and protopectin of granulated juicy sac were higher than those of non –granulated juicy sac respectively, but activity of Cx, activity of PE and activity of PG of granulated juicy sac were lower than of non-granulated juicy sac respectively.

纤维素酶活性在成熟期间随果实成熟上升，到9月8日出现活性高峰后下降；果胶甲酯酶活性在成熟阶段前中期(9月18日)PE活性呈下降趋势，在9月28日出现PE活性高峰后快速下降，汁胞PG前中期活性较低，9月18日后的变化趋势和PE活性的变化一致。粒化汁胞的纤维素，原果胶含量均高于未粒化汁胞；它的CX活性、PE活性，PG活性低于未粒化汁胞。

Fresh weight, dry weight, root activity, peroxides activity and catalase activity were measured. The results showed that FW, DW, root activity, POD activity and CAT activity of Vallisneria natans L and Elodea nuttallii were improved when ammonia concentrations were less than 50mgkg^(-1) and 500mgkg^(-1), respectively.

试验结果表明，氨氮起始质量分数＜50mgkg^(-1)和＜500mgkg^(-1)时可以分别促进苦草和伊乐藻鲜物质量、干物质量和根系活力的增加，大于此范围则会抑制苦草为Vallisneria natans L和伊乐藻Elodea nuttallii的生长。

Organize the activity of some of combination, attract a few people to come over to conduct card quality, use for instance impose fine, recommend wait for a method, newcome of reoccupy content tarry person for instance I am new personality, my award to imposing fine is more absorption than the award to the gift, breathe out ah the activity is actually much it is pretty good, but there is communication to just go between the mainest strengthen leaf fat, picture a few board piece do an activity, integral is good, only everybody sends each each, hair was over to go, when the activity ends, do not have a person all the same, hold an activity to do not have what meaning so after all some are full of stains or spots in forum take place only but however attempt and accomplish nothing just opened content of edition give sb a present to do organizing an activity is little not.

搞些联合的活动，吸引一些人过来引导帖子质量，比如用加精，推荐等手段，再用内容留住新来的人比如我是新人，我对加精的奖励比对礼物的奖励更热中，呵呵其实活动多了是不错，但是最主要的得加强板油之间有交流才行，像一些板块做活动，积分是好得，只是大家都各发各的，发完了就走了，等到活动一结束，照样没有人，这样举行活动就没有什么意义了毕竟论坛里有些斑斑只占位子但是却无所作为刚开版送礼物搞搞活动是少不了的。

Under the 6 h stress, SOD activity had been to maintain a high level, POD activity showed a greater change. When the stress lasted until 24 h, POD activity decreased and almost flatted compared with the control, this showed that POD activity was sensitive under the low temperature stress. Changes of CAT and SOD activity were in the line, the results indicated that activity of two enzymes coordinated the regulation of low-temperature adaptation.

本实验中，SOD活性一直保持较高的水平；POD活性表现为波动较大，当胁迫持续到24 h时，POD活性明显降低几乎与对照持平，说明POD受低温影响更敏感；CAT活性与SOD活性变化保持一致，表现出这两种酶在低温适应性调节过程中的协同作用。

The protease activity in foregut was higher than that of midgut in an acidic condition at pH 5.0-5.8, while in an alkalescence condition at pH 7.0-8.6, the protease activity was higher in midgut than that in foregut. The lipase activity of foregut was high and stable in two pH ranges: 4.2-5.0 and 6.2-7.0, and that of midgut was high at pH 3.8, while the lipase lost enzyme activity at pH over 9.0. The amylase activity was high and stable at pH 6.6-7.4 in both foregut and midgut. The cellulase activity of foregut and midgut was high and stable at pH 6.2-7.4 and 5.4-7.0 respectively.

刺参前肠蛋白酶活力在酸性环境下较中肠高，且在反应pH为5.0-5.8之间酶活力相对稳定，而中肠蛋白酶活力在碱性环境下较前肠高，且在反应pH为7.0-8.6之间酶活力相对稳定；刺参前肠脂肪酶活力随着pH的升高出现两个相对稳定的峰值，分别为4.2-5.0和6.2-7.0区间，中肠脂肪酶活力在pH为3.8时达到最大值，而在pH超过9.0明显失活；刺参前肠和中肠淀粉酶活力在pH变化时表现出相似的变化趋势，在反应pH为6.6-7.4之间酶活力较高且相对稳定；刺参前肠和中肠中纤维素酶活力在pH变化时反应不一致，前肠淀粉酶在反应pH为6.2-7.4之间酶活力较高且相对稳定，中肠纤维素酶活力在反应pH为5.4-7.0之间酶活力较高且相对稳定。

The PPO activity of genotype 2AL/2DL,2AL/2DH,2AH/2DL,2AH/2DH get higher one by one for one degree40-60AU/min·gand their activity level is low medium,medium,medium high,high medium,respectively;and they make activity decrease 30.1%,decrease 7.8%, increase 8.3%,increase 30.0%,respectively.8.2AL and 2DL gene make kernel PPO activity decrease 25%and 10%, respectively.2AH and 2DH gene make kernel PPO activity increase around 17%.2AL, 2DL,2AH,2DH keep PPO activity at low,medium,medium high,medium high level, respectively.

四种基因型2AL/2DL、2AL/2DH、2AH/2DL、2AH/2DH的籽粒PPO活性逐渐升高一个档次(40-60AU/min·g)，且其活性分别处于低偏中、中等、中偏高、高偏中水平，可分别使活性降低30.1%、降低7.8%、升高8.3%、升高30.0%。8.2AL和2DL基因分别使籽粒PPO活性降低25%和10%。2AH和2DH基因均可使籽粒PPO活性增加17%左右。2AL、2DL、2AH、2DH基因分别使籽粒PPO活性维持在低、中等、中高、中高水平。

Therefore, systemically research on distribution and activity of fault system in the middle part of Huanghua depression is done in this paper, including the quantitative analysis of planar distribution and activity of fault system ,the anatomization of the activity and evolution of the major fault, in order to determine the relativity and cause of the fault system. Conbined with the found reservoirs and the evidences of oil and gas displaying .The relationship between fault activity time and activity characteristic, planar distribution and the displacement type of the fault system, the contact relationship of lithology with the formation of oil and gas pools is discussed and finally the faults developing model to form oil and gas pools of upper Tertiary system are found in middle part Huanghua depression.

本论文以黄骅坳陷中区为主系统的研究了断裂系统的展布和活动特征，包括断裂平面展布与活动的定量分析、主干二级断层的几何特征和活动演化历史的详细解剖，来分析断裂系统的相关性和断裂系统的成因，并与黄骅坳陷已发现的油气藏及已有的油气显示成果结合，探讨断层的活动时间和活动特点、断层的平面分布、平面和剖面组合关系、断层两盘的岩性对接关系等方面与油气成藏的关系，最终建立黄骅坳陷中区新近系油气成藏的断裂发育模式。

Fourth, the work of netizens in Changsha.

四、在长沙工作的网友。

You are unique, Milo, unlike any I have ever seen before.

你总是充满希望的微笑着，仁慈地对待每一个人，即使是你要杀的人。