查询词典 actinomycin

The influence of the substrate concentration, temperature, pH value, the concentration of certain inorganic ions(Mg, Mn, NH4 +), actinomycin D and α-amanitin on the polymera

观察了底物浓度、温度、pH等与转录活性的关系；探讨了Mg~、Mn~、NH_4~+等离子和放线菌素D、α-Amanitin对转录活性的影响。

Methods Microsatellite instable-high colon cancer cell line HCT116, microsatellite stable colon cancer cell line HT29, as well as control MSS breast cancer cell lines were treated by NMD inhibition drug emetine and actinomycin D.

方法采用NMD抑制药物emetine联合actinomycinD，分别处理高度微卫星不稳定型大肠癌细胞株HCT116和微卫星稳定的大肠癌细胞株HT29，以及对照的MSS乳腺癌细胞株，以全基因组cDNA芯片检测经药物处理和不经药物处理的细胞株的mRNA表达变化。

Nuclear Run-on Assay was used to address the contribution of transcriptioninitiation to the regulation of five histones by YSV. Actinomycin D inhibitor studies were used to detect the mRNA half-life of five histones. SLBP weremeasured by Western blot assay to identify regulation of YSV on Pre-mRNAsplicing.

应用Nuclear Run-on Assay观察YSV对人肝癌BEL-7402细胞的五种组蛋白转录效率的影响；应用放线菌素D转录抑制法观察YSV对组蛋白mRNA半衰期的影响；应用Western blot检测SLBP的表达水平观察YSV对组蛋白mRNA加工的影响。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

In addition, a metabolic inhibitor, actinomycin D has no effect on enzymatic activities of caspase-1/ICE-like and caspase-3/CPP-32/like proteases during Fas-mediated CD4〓T cell apoptosis. These results indicate that the appearance of caspase-1/ICE-like and caspase-3/CPP32-1ike proteases activities is a post-translational activation of these proteases. It is likely these caspases constituently express in a inactive form in the cytosol of CD4〓T lymphocytes. Once the rest SEB-responsive CD4〓T cells are restimulted, those inactive caspases such as caspase-1/ICE-like and caspase-3/CPP32-1ike proteases are sequentially cleaved to become active forms, and the apoptotic signal is transducted to downstream signaling molecules, Finally leads to CD4〓T cells apoptosis.

另外，通过代谢抑制剂放线菌素D对超抗原SEB诱导静息SEB应答〓细胞凋亡过程中Caspase成员酶活性变化的研究表明Caspase-1／ICE样，Caspase-3／CPP32样胱氨蛋白酶并非新合成的信号分子，很可能以无活性方式组成性表达于静息SEB应答〓细胞胞浆，在超抗原SEB再次刺激诱导〓细胞凋亡早期，先后顺序活化，借以传递凋亡信号到下游信号分子，诱导〓细胞凋亡。

The colon temperatures were measured by a thermistor and the cytosolic free calcium concentration ([Ca2+]i) in dissociated brain cells was measured by Fura 2-AM. The results demonstrated that the pyretic action of egtazic acid (0.6 μmol, icv) was markedly inhibited by DEX (5 mg/kg,iv), but DEX (60-120 μmol/L) did′t affect [Ca2+]i in dissociated hypothalamus cells. Actinomycin D, which interferes with gene transcription (3 nmol, icv), completely abolished the antipyretic action of DEX on egtazic acid-induced fever.

结果发现：脑室灌注乙二醇双(2-氨基乙醚)四乙酸(0.6 nmol)引起家兔结肠温度明显升高，静脉注射地塞米松(5 mg/kg)显著抑制家兔乙二醇双(2-氨基乙醚)四乙酸性发热，地塞米松(60-120 μmol/L)并不影响下丘脑细胞内[Ca2+]i，而事先脑室灌注抑制基因转录的放线菌素D(3 nmol)则完全取消了地塞米松对乙二醇双(2-氨基乙醚)四乙酸性发热的解热作用。

At this time,priming with 25 IU/ml mouse IFN about 12-24 h and then induced with NDV and superinduced with cycloheximide(20μg/ml) and actinomycin D(2μg/ml),the titer of IFN in the media would be 10~5IU/ml approximately, or 10~5IU...

用25IU/ml鼠IFN起动12—24小时，用NDV作诱生剂，并采用环己亚胺(20μg/ml)和放线菌素D(2μg/ml)进行超诱导，IFN产量可高达1×10~6IU/ml左右，比活接近1.3×10~5IU/ml蛋白。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。