查询词典 actin

The results showed that when both β-actin and GAPDH were used as internal control the MYH mRNA expression was complete consistent, and its expression was increased from the gastrula stage to larval stage, which represented the statistic significant difference at the level of 0.05 possibility. However, the level of mRNA expression during the developmental stages was instable when 18S rRNA was used as internal control gene.

研究结果表明，以β-actin和GAPDH作为内参，MYH基因mRNA表达水平完全一致，其表达量从原肠到仔鱼阶段逐次递增，仔鱼与原肠期阶段相比表达量差异显著；当采用18S rRNA作为内参时，MYH基因mRNA在发育阶段的表达量呈不稳定状态。

The coding sequence of grouper Epinephelus coioides growth hormone gene was cloned under the regulation of carp β-actin gene promoter and enhancer, so an"all-fish"recombinant pCAecGHc was constructed, then the construct was microinjected into eggs of Lepomis cyanellus and the transgenic Lepomis cyanellus was produced.

通过基因重组，将石斑鱼生长激素基因编码序列克隆到鲤鱼β-actin基因启动子下游，构建了&全鱼&生长激素基因表达载体pCAecGHc。

Actin plays an important role in the pathogenesis of nonbacterial chronic prostatitis.

β？actin作为紧密连接中的关键性调节因子，在慢性无菌性前列腺炎的发病过程中可能具有重要的调节作用。

As the critical regulatory factor of tight junction,βactin plays an important role in the pathogenesis of nonbacterial chronic prostatitis.

βactin作为紧密连接中的关键性调节因子，在慢性无菌性前列腺炎的发病过程中可能具有重要的调节作用。

Actin in nonbacterial chronic prostatitis model rats before and after immunosuppressive therapy, and to evaluate its correlation with CAP.

actin在大鼠慢性无菌性前列腺炎（nonbacterial chronic prostatitis，CAP）动物模型免疫抑制治疗前后的表达，探讨其与慢性无菌性前列腺炎发病机制的相关性。

AIM: To investigate the expression of βactin in nonbacterial chronic prostatitis model rats before and after immunosuppressive therapy, and to evaluate its correlation with CAP.

目的：检测βactin在大鼠慢性无菌性前列腺炎（nonbacterial chronic prostatitis，CAP）动物模型免疫抑制治疗前后的表达，探讨其与慢性无菌性前列腺炎发病机制的相关性。

The blots were probed with anti-Cdx-2, anti-JNK (Santa Cruz Biotechnology, Santa Cruz, California), anti-ERK, anti-phospho -ERK, antip38MAPK,anti-p-p38MAPK, anti-p-JNK (Cell Signaling, Beverly, Massachusetts) and anti-actin (Amersham Life Sciences,Piscataway, New Jersey) antibodies, and then incubated with appropriate species specific secondary antibodies labeled with horseradish peroxidase.

Blots分别与抗CDX-2抗体，抗JNK抗体（圣克鲁斯生物技术，圣克鲁斯，加州），抗ERK抗体，抗phospho -ERK抗体，抗p38MAPK抗体，抗p-p38MAPK抗体，抗p-JNK抗体（Cell Signaling， Beverly，美国马萨诸塞州）和抗actin抗体（ Amersham生命科学，皮斯卡塔韦，新泽西州）作用，然后在与辣根过氧化物酶标记的第二抗体孵育。

Methods The pathological features of EPS were observed in 17 cases, and the expressions of HCG, HPL, EMA, PRL, PLAP, Vim, actin, c-erbB-2 and c-myc were analyzed immunohistochemically.

对17例经病理检查诊断为EPS的病例进行病理学形态观察，采用免疫组化方法标记滋养细胞HCG、HPL、EMA、PRL、PLAP、Vim、actin、c-erbB-2和c-myc。

In our laboratory a chemically regulatable male sterile expression system had been constructed based on the antisense actin strategy and putatively transgenic tobacco plants with Kan-resistance had been obtained.

本实验室在自行研究成功的反义肌动蛋白雄性不育策略的基础上，构建成由铜调控的在绒毡层细胞特异表达anti-actin基因的植物表达系统，转化烟草获得由kan抗性筛选的烟草植株。

TUNEL method was used to detected the apoptosis in the artery. Results:①The MSC cocultured with VSMC expressed smooth muscle a-actin, calponin and CD31, no cells positive for calponin and CD31 were detected in the control group; and a lot of filaments were observed in the co-cultured MSC by electron microscopy.②We gain dual-stable expression of AT2R gene medicated by doxycycline regulatable system of mesenchymal stem cells.

单独培养及VSMC条件培养液培养的MSC仅SM-α-actin表达阳性；(2)Dox-on系统由四个质粒组成：调节质粒pUHD17-1 hyg，荧光报告质粒pUHC 13-3，筛选质粒pSV2neo以及含目的基因AT2R的应答质粒pUHD10-3/AT2R，以上质粒经鉴定均与其背景资料相符合，可用于实现目的基因片段在靶细胞中可调控表达研究；(3)本实验通过连续两个回合的转染及抗生素压力筛选，经Dox诱导表达后获取出低背景、高诱导表达AT2R基因的克隆。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。