查询词典 actin

To investigate the variations of actin and ezrin protein and the interference of phosphocreatine of newborn porcine RTC ...

目的：研究缺氧新生猪RTC细胞actin及ezrin蛋白的变化和磷酸肌酸的干预，探讨新生儿窒息肾功能损伤的发病机制。

To investigate the variations of actin and ezrin protein and the interference of phosphocreatine of newborn porcine RTC cell during hypoxia, and discussion the mechanism of renal deficiency of newborn asphyxia.

目的：研究缺氧新生猪RTC细胞actin及ezrin蛋白的变化和磷酸肌酸的干预，探讨新生儿窒息肾功能损伤的发病机制。

Locations of transgenes in the recovered plasmids were determined by southern blotting. And then adjacent sequences of transgenes integration sites in three recovered aberrant classes were subcloned and analyzed. Transgene of A-2 was inserted into common carp DNA sequences homologous to the mouse phosphoglycerate kinase-1 gene and those of A-3 and A-6 were inserted into common carp DNA sequences homologous to the human epidermal keratin 14 gene and sequence of common carp β-actin gene, respectively.

通过Southern杂交对转植基因在回收质粒上的位置进行了定位分析，并对三种回收到的变异型转植基因旁侧顺序进行了亚克隆和顺序测定。A-2的转植基因整合到了与小鼠磷酸甘油酸盐激酶-1基因同源的鲤鱼基因组顺序旁侧，A-3的转植基因整合到了与人表皮角蛋白14基因同源的鲤鱼基因组顺序旁侧，A-6的转植基因整合到了普通鲤鱼β-actin基因顺序中。

8 cases of dendritic fibromyxolipoma were obtained and their clinicopathologic features were studied. Immunohistochemistry stains for CD34, bcl-2, Vimentin, Cytokeratin, EMA, S-100, HHF35 and Smooth Muscle Actin were performed with SLAB system on DAKO Auto-immunohistochemical stainer.

收集8例树突状纤维粘液脂肪瘤，观察和分析其临床和病理组织学特征，并通过免疫组织化学 LSAB 法，分析其 CD34、 bcl-2、 Vimentin 、 Cytokeratin 、 EMA 、 S-100、 HHF35和 Smooth Muscle Actin 等的表达。

The results suggest lineage-specific β-actin loss might happen in the radical evolution of teleost fishes.

这暗示伴随着鱼类的辐射式进化历程，可能发生了种系特异性的β-actin的丢失。

Methods With β-actin gene as a reference, reverse transcriptase polymerase chain reaction was conducted to dectect the expression of PSA mRNA in 35 BC samples, 12 paracarcinoma breast tissues and 10 breast adenofibroma samples.

以β actin作为内部参照半定量RT PCR方法检测 35例乳腺癌组织、12癌旁乳腺组织和 10例乳腺纤维瘤中PSAmRNA的表达。

Using phalloidium staining we observed a sharp disappearance of the actin stress fibers, formation of extensive microvilli as demonstrated by the scanning microscopy and formation of typical bile canaliculi structures as confirmed by a monoclonal antibody to aminopeptidase N which is a BC specific antigen. The appearance of microvilli and BC suggested that selenium is able to elicit a differentiation reprogramming.

利用Rhodamine pholloidin 染细胞内的F-actin 显示出细胞内的stress fiber 大部分都消失；利用Scanning electron microscope可以检测到细胞表面有明显的microvilli 的结构；并且利用标定胆小管的特殊蛋白可以发现硒元素可以诱导HuH7 肝癌细胞分化产生胆小管的结构。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Our objective is to understand the effect of the different epileptogenic matter as penicillin, coriaria lactone, bicuculline on the expression of hippocampus neuron microfilament F-actin and the relation with the gap junctional communication , and explore their significance in the epileptogenesis.

本实验旨在了解致痫剂如：青霉素、马桑内酯、荷包牡丹碱对海马锥体神经元细胞骨架微丝肌动蛋白F-actin的影响，探讨该表达与神经元缝隙连接通道的关系以及它们在癫痫发病中的意义。

The remaining 9 rats in control group, 16 rats in treated grouop and 15 rats in model group were decollated and obtained brains to detect the relative mRNA expression of nuclear receptor-related factor 1 (Nurr1) and muscarinic acetylcholine receptor 5(M5) byusing RT-PCR, which was the consequence of those comparing with reference geneβ-actin mRNA expression.

将对照组、治疗组、模型组其余9、16、15只大鼠，断头取脑，采用RT-PCR方法检测大鼠脑组织孤儿核受体及乙酰胆碱毒蕈碱型受体5(M5)mRNA相对于内参基因β-actin mRNA的相对表达值。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。