查询词典 actin

A new expression vector with the promoter of the medaka β-Actin promoter and green-fluorescent protein gene was constructed based on Nde I site of pBluescript SK+.

从载体pBluescript SK+的Nde I位点处插入外源基因片段，构建了含有青鳉β-肌动蛋白启动子和绿色荧光蛋白标记基因的新表达载体。

Among seven different constructions we examined, a DNA fragment, named pCMV-MB-EGFPITR, containing EGFP reporter gene driven by human cytomegalovirus promoter and medaka β-actin promoter, produced the most intense GFP expression.

在我们试验的七个不同构筑质体中，被命名为pCMV-MB-EGFPITR的DNA片段，含有被人类巨细胞病毒启动子和稻田鱼β型肌动蛋白启动子所驱动的增强型绿萤光蛋白报导基因，能产生最强的绿萤光表现。

We employed a cell line of mouse podocytes immortalized with the simian virus 40 large tumor antigen gene, and examined the cell proliferation using colony-forming and MTT assays following treatment with various concentrations of bFGF. BMP-7, alpha-smooth muscle actin e podocytes were detected by western blot. Methyl blue staining was used for the observation of cell morphology.

利用SV40肿瘤抗原转染所获得的永生性小鼠足细胞系培养，以集落形成法和噻唑蓝MTT还原实验测定不同浓度bFGF刺激下的足细胞增殖，采用SDS-聚丙烯酰胺凝胶电泳和Western blotting 检测BMP-7、alpha-平滑肌肌动蛋白、以及波形蛋白的蛋白质水平表达，同时以甲基兰染色观察细胞形态。

Using semi-quantitative PCR within the exponential phase, with beta-actin as an external control, the constitutive transcriptional expression of genes of phase I (CYP1A), phase II xenobiotic metabolizing enzymes, HSP70 and ATP1A1 were also investigated.

应用半定量RT-PCR的方法，以β-肌动蛋白作为外参照，在指数期增长范围内分别得到了CYP1A、GSTA、GSTR、HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA的比值，确定黄斑篮子鱼肝脏去毒相关基因的组成型表达水平。

The method has thyroid transcribe factor to specimen of 12 SHL tissue - antigen of 1(TTF-1), epithelial film , CK7, CK20, dash forward the albumen that touch bubble , undee albumen , flowing flesh actin , placental alkalescent phosphoric acid is enzymatic , be addicted to bead albumen, among them observation of 10 lens that make report.

方法对12例SHL组织标本进行甲状腺转录因子-1(TTF-1)、上皮膜抗原、CK7、CK20、突触泡蛋白、波形蛋白、平滑肌肌动蛋白、胎盘碱性磷酸酶、嗜铬粒蛋白、CD34的SP免疫组织化学法标记，其中10例做电镜观察。

DNA synthesis rate was assessed by performing tritiated thymidine incorperation assay. The expression of SM-α-actin and proliferation of DSMC were analyzed by immunofluorescent assay and flow cytometory.

结果显示，逼尿肌细胞的形变量和粘弹性并不呈线性关系，一定限度内的张力负荷（拉伸20％）粘弹性系数下降，而继续加大张力（拉伸30％）时变化并不明显。

Results From the fourth week, in diabetic rats, the contents of AGEs in plasma and the abdominal aorta increased, the relative area of α-SM-actin in the abdominal aorta decreased, the density of nucleus of VSMC increased, and mitochondria and rough endoplasmic reticules in VSMC increased.

结果 从4周起，DM大鼠血浆和腹主动脉的AGEs显著增多；腹主动脉α-SM-肌动蛋白的相对面积减小，VSMC的细胞核密度增大，线粒体和粗面内质网增多。

Methods The changes of contents of AGEs in plasma and the abdominal aorta and changes of VSMC of the abdominal aorta were investigated with methods of fluorospectrophotometry, immunohistochemistry, computer image analysis and transmission electron microscopy on streptozotocin-induced diabetic rats. Results From the fourth week, in diabetic rats, the contents of AGEs in plasma and the abdominal aorta increased, the relative area of α-SM-actin in the abdominal aorta decreased, the density of nucleus of VSMC increased, and mitochondria and rough endoplasmic reticules in VSMC increased.

后者在早期主要是血管平滑肌细胞(vascular smooth muscle cell， VSMC)和胶原纤维增多，弹性纤维减少，动脉增厚等，引起动脉的顺应性降低，舒张功能减弱［1～3］；晚期主要表现为动脉粥样硬化和动脉硬化，其中以VSMC的增生和迁移较为突出，其表型也由收缩型转化为合成型［4，5］。

To get your brain relaxin, cause they be actin maniac in action

放松你的大脑，使他们在行动中发狂

After removing DNA, histones and part of NHPs, we found that actin and tropomyosin exist in the polytene chromosomal scafiold by immunofluorescence experiments.

对去除DNA、组蛋白和大部分非组蛋白的果蝇多线染色体的免疫荧光实验表明，肌动蛋白和原肌球蛋白存在于果蝇多线染色体骨架上。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。