查询词典 actin

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

RESULTS: A total of 36 rabbit models, 6 in the 1-month therapy group and 8 in the 3-month therapy group were died from infection. In the 1-month therapy group, thick ureteric wall and smooth ureteric lumina developed without any contracture. Under the optical microscope, transitional epithelial cells covered throughout the inner walls and smooth muscle cells appeared sparsely in a mussy configuration. In addition, CKAE1/AE3 staining was positive butα-actin staining was very weak when checked by immunohistochemistry. In the 3-month therapy group, amnion-ureters were enclosed by their peripheral tissue and showed rich blood vessels and normal ureteric lumina without significant contracture, futher more, there had formed a well-arranged transitional epithelial layer and smooth muscular layer, the immunohistochemistry result showed that the expression of CKAE1/AE3 and α-actin were positive.

结果：36只大白兔中，1个月治疗组死亡6只，3个月治疗组死亡8只，死亡原因均为感染。1个月治疗组大白兔羊膜输尿管柔软，长4 cm，管壁变厚，无挛缩，移行上皮组织爬满内壁，平滑肌组织部分再生，但较稀疏，排列不规则；免疫组织化学检查提示CKAE1/AE3染色阳性，α-Actin染色微弱。3个月治疗组大白兔羊膜输尿管与周围粘连，有丰富的血运，无明显挛缩，管腔不缩小，已形成良好的移行上皮层和平滑肌层，免疫组织化学显示α-Actin和CKAE1/AE3染色均为阳性。

Phylogenetic trees including all β-actin cds or 3 UTR of teleost fishes were constructed respectively, which indicate that teleost β-actin can be classified into four types, and the β-actin cloned from the rice field eel here is type II.

基于目前已知的全部鱼类的β-actin cds和3'UTR，我们分别构建了进化树。结果它们都一致支持将鱼类的β-actin基因划分为四类。黄鳝的β-actin基因属于其中的第二类。

The staining of F-actin in the ischemia reperfusion group was decreased significantly （P.05） compared with the normal control group. There was a marked loss of canalicular microvilli and distension of canalicular lumen in the ischemia reperfusion group. The staining of F-actin in the LA group was significantly increased than those in the I/R group （P.05）. Conclusions: Ischemia reperfusion induces an disruption of bile canalicular F-actin microfilaments and a loss of microvilli. Alpha-lipoic acid can protect bile canalicular F-actin microfilaments against IRI.

肝脏I/R可造成胆小管F-actin微丝破坏、微绒毛丧失，导致胆小管收缩减弱，胆汁排泄功能受损，这可能是大鼠肝脏I/ R后缺血型胆道病变发生的主要机制；α-硫辛酸通过清除氧自由基，降低脂质过氧化反应，调节细胞内氧化剂-抗氧化剂的含量，保护胆小管F-actin微丝结构免遭缺血再灌注损伤破坏，进而预防和治疗缺血型胆道病变。

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鲤科鱼类Beta-肌动蛋白基因序列，采用长片段PCR技术，克隆了青鱼β-actin基因DNA片段，并构建了青鱼β-actin基因5'启动调控区绿色荧光蛋白表达载体，通过显微注射泥鳅胚胎和COS-7细胞转染实验，证明了所克隆的青鱼β-actin基因5'启动调控区具有启动调控功能，并用之来构建&全鱼&基因的启动子部分。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

Mmunohistochemically, the tumor cells of both phenotypes in all three lesions stained for melanocytic (HMB-45 and Melan-A/MART-1) and myoid (desmin, smooth muscle actin, and muscle-specific actin/all muscle actin/HHF-35) markers.

HC，三个部位病变的2种肿瘤细胞表达HMB-45 and Melan-A/MART-1，肌样标记(desmin， smooth muscle actin， and muscle-specific actin/all muscle actin/HHF-35 。

In addtion to, Talin nucleates actin assembly and binds to vinculins and a host of actin binding proteins and other proteins mainly at the focal adhesion.

Talin在帮助actin的装配同时还可以和Vinculin及其它的actin结合蛋白以及粘着斑上的蛋白相结合。

It was demonstrated that the actin could be polymerized into discrete filaments of tree-like branch structure, random coil filaments cluster and long filaments with different diameters in F-buffer besides random aggregates. These polymerized filaments clearly exhibited the structural polymorphism and showed obvious difference from those assembled under the regulation by actin binding proteins or phalloidin.

研究发现，actin在体外通过自装配过程除了形成无序的蛋白堆积物之外，还能够聚合形成复杂的离散结构，包括树状分支的纤维丛、无规卷曲的纤维簇以及具有不同直径的长纤维等；这些大尺度纤维复合物明显不同于在ABPs或过量F-actin稳定剂参与下形成的由单根微丝和微丝束构成的聚合结构。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失