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The cutoff value of E2/E6, used to distinguish the pure episomal form from a mixed form of episomal and integrated HPV16 DNA, was 0.81 in the multiplex real-time PCR test. c） HPV16 existed as episomes in most of the CIN Ⅰ lesions, and 42.9% of them had mixed episomal/integrated HPV16. The integrated form of HPV16 constituted the majority in most of the CIN Ⅱand CIN Ⅲ cases. In squamous cervical carcinomas, the HPV16 was mostly integrated into the host chromosome. d） The rate of HPV16 integration was associated with the degree of cervical lesions and the rate increased with the progression of cervical disease, e） The rate of pure integrated HPV16 in stage Ⅱ and Ⅲ（88%） was higher than that in stage Ⅰ（33.3%）. Conclusions: a） Multiplex real-time PCR is a rapid and sensitive method for detection of the integration state of HPV16 DNA. b） The integration of HPV16 DNA is a very early event in the development of cervical cancer and may act as an activation mechanism for progression from preinvasive to invasive cervical cancer, c） The pure integrated HPV16 in cervical cancer may be a useful prognostic indicator.

HPV—16E2、E6标准曲线相关系数均为1.00，扩增效率均在95％以上：2）确定0，81作为多重实时PCR区分游离型和混合型HPV-16的界值、3）CINⅠ中HPV—16大多以游离方式存在，但仍有整合的发生（42，9％）；CINⅡ和CINⅢ中HPV—16以混合型为主：浸润癌中以整合型HPV—16为主。4）HPV-16整合发生率与不同程度的宫颈病变有关，并且随着宫颈病变级别的升高HPV-16整合发生率呈上升趋势-5）Ⅱ＋Ⅲ期宫颈癌中整合型HPV—16所占比例显著高于Ⅰ期宫颈癌结论：1）多重实时定量PCR是一种敏感性高、简便快速的HPV—16病毒存在状态的检测方法2）HPV-16整合是宫颈癌变的早期事件，与宫颈癌前病变的进展有关。3）宫颈癌中整合型HPV—16的发生可能具有预后价值

Rotary International will act to secure excess travel medical insurance for the district governor-elect and spouse for coverage traveling to, from, and while at the International Assembly. This coverage is intended to respond in the event of a catastrophic illness or injury.

国际扶轮替地区总监提名人及其配偶往返国际讲习会及其会议期间之旅行范围可能发生的重大灾难疾病及伤害而保了额外的旅行医疗保险。

Besides, there are many other exciting acts for your choice ,for example, Air Wire Act,Wedding of Miao Nationality, Bamboo Rod Dance, High-speed Motoboat,Air Suspension Bridge, Lido, Courser Gallop, labyrinth, Air Swing, Fishing, Tennis Ball, and mini garden, white tower, Hui-Style Bonsai, Float Paradise, Nine-Curve Love Bridge.

除这些景点以外，还有许多参与性项目可供选择，如：高空溜索、苗族抢亲、苗族竹杆舞、高速摩托艇、百米高空吊桥、露天游泳池、骏马驰骋草原、迷宫、高空荡秋千、恩龙湖垂钓、网球健身等等。还有园林小品、白塔、徽派盆景园、水上乐园、九曲情人桥让您驻足小憩。

Motherfukkers wanna act loco, hit em wit, numerous shots with the fo'-fo' Faggots runnin to the Po-Po's, smoke em like cocoa Fuck rap, coke by the boatload Fuck dat, on the run-by, gun high, one eye closed Left holes through some guy clothes Stop your bullshittin, glock with the full clip Motherfukkers better duck when the fool spit One shot could make a ***** do a full flip See the ***** layin shocked when the bullet hit I hate my high youth, no niggaz wanna buy you But see me I wanna fuck for free Now I gotta let her take this ride, make you feel it inside your belly, if it's tight get the K-Y Jelly All night get you wide up inside the telly Side to side, til you say Jay-Z you're too much for me I am a little bit of loneliness A little bit of disregard Handful of complaints But I can't help the fact That everyone can see these scars I am what I want you to want, what I want you to feel But it's like no matter what I do I can't convince you to just believe this is real So I let go watching you Turn your back like you always do Face away and pretend that I'm not But I'll be here 'cause you're all that I got Got a condo with nothin but condoms in it The same place where the rhymes is invented So all I do is rap and sex, imagine how I stroke See how I was flowin on my last cassette?

motherfukkers不愿扮演买卖，击中在束手无策，无数镜头与fo'佛' faggots runnin向蒲圃的，烟在想可可他妈的说唱，焦炭，由船他妈的数据，对我们来说处，枪高，睁一眼闭一眼，左孔，通过一些家伙衣服停止你的bullshittin ，格洛克与充分剪辑motherfukkers更好的鸭当傻瓜随地吐痰一杆可以做出*****做了充分倒装看到***** layin震惊时，一颗子弹击中我恨我高青年，没有niggaz想购买，但你看我，我想他妈的免费现在，我的宝贝，让她在此转乘，使你感到这里面你的肚子，如果它的紧迫得到健扬果冻一整夜，你得到了广泛的内telly方方， TIL的，你说的Jay - Z你太多，我我感到一点点的寂寞一点点的漠视少数投诉不过，我可以帮不了事实，即每个人都可以看到这些伤疤我什么我要你想，我想你的感觉，但它的，不管是什么，我我无法说服你只是相信这是真实的，所以我放手看着你谈谈你的背部一样，你总是做脸去，假装我不是但是，我可以在这里'的事业，你都是我的一个共管与nothin但是避孕套在这同一个地方，而语言是发明所以，我要做的就是Rap及性别，想象我如何中风，看看如何，我flowin对我的最后卡式？

An adaptation he had been assigned to do for the famous actress Lana Turner was rejected as unsuitable for her; Williams described Turner in his Memoirs as unable to act her way out of her form-fitting cashmere.

&适应他被分配到这样的著名演员拉娜特纳被否决不适合她；威廉姆斯描述特纳在他的回忆录为无法&行事她走出她合身的羊绒。

When the document is delivered to the office of the sectary of state for filing, the correct filing fee, and any franchise tax, license fee, or penalty required to be paid therewith by this Act or other law must be paid or provision for payment made in a manner permitted by the secretary of state.

j 当文件提交州务卿办公室时，必须支付或者以州务卿允许的方式提供适当的备案费以及依据本法及其他法律规定要求交付的任何特许税、执照费或者罚金。

The results suggested that a low content of TCB and NAP would significantly influence the growth of rice seedling,presenting an important suggestion for water quality management during rice seedling period,and root length could act as an inference plant morphologic indicator for organic incident of TCB or NAP.

研究结果显示，水中微量的1，2，4-三氯苯和萘均会明显影响水稻幼苗的生长，这对水稻育秧期水质管理具有重要的参考意义，同时，根长可作为确定有机污染物发生事件的植物形态学参考指标。

Of course, also a few players express, for " lasting tower " activation code, they are willing to act according to on oneself love that breaks a back for the first time... hard bubble of use soft tactics who cannot sustain is ground,"Grind " child part is first the condition is face wants thick, before using this device, you are OK steal division to the sale of insurance company, clerk that sells promotion company continuously first.

当然，也有一些玩家表示，为了《永恒之塔》的激活码，他们愿意奉上自己的第一次断背之爱……软磨硬泡谁都架不住磨，&磨&子诀首要条件便是脸皮要厚，使用这招前，你可以先向保险公司的销售、直销推广公司的业务员偷师。

Because of above, I decided to write the dissertation with the heart of logistics insurance, the main point for the dissertation is that: no matter who dont know it will get it, people who engage insurance will know how to act in logistics insurance and how to solve problem, management level of insurance will know how to improve it and make it suitable for development of logistics, insurants read it will know how to choose a employable policy company.

基于上述情况，本文以物流保险为核心，写此论文的主导思想是：凡是没有做过保险的人看过此文，明白什么是物流保险；从事保险工作的人看过此文，明白如何做物流保险，怎么解决实务中的难题；保险管理者看过此文，明白如何完善现有的物流保险，适应当今物流业的发展；被保险人看过此文，明白如何选择一个好的保险人。

Or when you start a small local newspaper written by you and your friends and whose copies are sold or even given away free, for example a simple newspaper to discuss the music that you love (in America, we call these newspapers "zines"), you learn how providing a forum for discussion can unite a group of people and have a direct impact on the way they think and act.

当你创办一份由你自己及朋友撰文的地方小报并销售或无偿发送时，譬如一份探讨你们喜欢的音乐的小报纸(在美国，我们称之为&××爱好者报&)，你会了解到通过提供这样的一个论坛，你可以把很多人团结在一起，并且可以直接影响他们的言行。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。