查询词典 acridinium

Four peaks of acridinium of 2 showed pronounced upfield shifts due to the conversion of acridinium to acridane in 5 equiv of either F-(Figure 3b) or AcO-(Figure 3c).

对于与中心吡啶环相邻的质子（ΔHa=1.48ppm）以及相对于吡啶的氮的对位质子（（ΔHc=1.47ppm）观察到了最大的化学位移。

The addition of F-/AcO- to 2 was further confirmed by monitoring the changes in its 1H NMR spectra in the presence of either F- or AcO-(Figure 3). Four peaks of acridinium of 2 showed pronounced upfield shifts due to the conversion of acridinium to acridane in 5 equiv of either F-(Figure 3b) or AcO-(Figure 3c).

F－/AcO－添加到2中通过监测其在存在F－或AcO－时H NMR谱的变化进一步得到确认（图3）。2的吖啶盐的四个峰值证明了由于在5个当量F－（图3b）或AcO－（图3c）中吖啶盐转化成吖啶而引起的明显的高场位移。

In chapter 2, we introduced the fabrication of two kind of CE-CL interfaces, which are able to be used for the detection of Acridinium ester and its' labeled analytes.

论文的第二部分（包括第2、3、4章）主要涉及毛细管电泳化学发光检测器的研制及应用研究。

To explore the clinical reliability and usability of the acridinium ester-labeled CLIP and ELISA in detection of AFP to establish a specific, effective, simple and reliable detecting method in our lab.

［目的］探讨吖啶酯标记的化学发光免疫测定(chemiluminescence immunoassay， CLIA)分析技术与酶联免疫吸附试验一步法两种方法检测甲胎蛋白(Alpha-fetoprotein， AFP)的临床可靠性、适用性。建立本室特异、有效、方便、可靠的试验方法。

The disappearance of the three characteristic absorption peaks corresponding to the acridinium salts and the newly formed peaks at 280 nm for 1 and 288 nm for 2 strongly suggested transformation of the acridinium moiety into the corresponding acridane via anionic addition to the C9 position of the former (Figure 2b).

与吖啶盐相应的三个特性吸收峰的消失以及新形成的峰值（1为280nm，2为288nm），强烈表明了吖啶盐分子部分通过阴离子加入到前者的C9位置而转化成了相应的吖啶（图2b）。

CLIA using acridinium ester derivatives as chemiluminescent label has advantages of low background,high signal-to-noise ratio,no need of catalyst and simple luminescence system.

以吖啶酯衍生物为发光标记物的CLIA的特点是：标记简单、标记物稳定、不需要催化剂、自然本底低、受外界干扰因素少、信噪比高、发光体系简单等。

These chemical shifts in the aromatic region of the resulting nucleophilic adduct 2-F were consistent with the counterparts of 9,10-dihydro-9-methoxy-9-(4-methoxyphenyl)-10-methylacridine, 17b which was proven to be the adduct of the 9-phenyl acridinium salt formed with an excess of MeOH (Figure S7 in Supporting Information).

在最终的亲核加成化合物2-F的芳香族区中的这些化学位移与9，10二氢-9-甲氧基-9-(4甲氧基苯基)-10-甲基吖啶的对应物是一致的，17b 这被证明是与过量的MeOH形成的9-苯基吖啶盐的加成化合物（支持信息中的图S7）。

In the two-site sandwich CLIA for TSH,two anti-TSH monoclonal antibodies were involved,one was coated on microwells and another was labeled with acridinium ester. The assay sensitivity is 0.01mlU/L. Intrassay and interassay coefficients of variation are 4.29-6.71 and 4.36-6.14%,respectively. Average recovery is 98%.

以 DMAE·NHS为发光标记物，研究建立了两种 TSH CLIA方法，均采用一步夹心免疫分析法，在第一种方法中一株抗TSH单克隆抗体包被微孔板，另一株标记DMAE·N'HS，方法的分析灵敏度为0.olmIUA，批内变异系数为 4.29巧。71％，批间变异系数为 4.36ed.14％，平均回收率为 98％，与 TSH IRMA法的相关方程为 Y－0.20＋0.92X，相关系数为 0.993，与 Ciba Co讪ng TSH CLIA测量值的相关方程为 Y—0.16＋0.92兄相关系数为 0.986。

Different aspects associating the DMAE-NHS-based CLIA were studied in this thesis,including synthesis of acridinium ester,chemiluminescent characteristics of DMAE-NHS,labelling antibody or streptavidin with DMAE-NHS,two-site sandwich chemiluminescent immunoassay for TSH,two-site sandwich chemiluminescent immunoassay involved biotin-streptavidin system for TSH,and competitive chemiluminescent immunoassay using biotin-streptavidin system for TLVThe desired acridinium ester,DMAE-NHS,was synthesized according to the reference method with some modifications.

目前国内尚未见有关DMAE·NHS的合成及其应用于CLIA的研究报导，本论文研究了吖啶酯DMAE·NHS的有机合成方法，DMAE·NHS的发光性能，DMAE·NHS及其与抗体偶联物的热稳定性，DMAE·NHS标记TSH单克隆抗体和链亲和素，TSH CLIA，TSH BAS-CLIA和TT_4 BAS-CLIA。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失