查询词典 accumulator cell

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results: T cell anergy was antigen-specific. The state of T cell anergy can be reversed by PHA, CD3 mAb and PMA plus A23187. IL-2 can prevent the induction of T cell anergy, but it can not reverse the state of un-responsiveness. IL-2 and IFN mRNA can not express in anergic T cells. In contrast, IL-4 and IL-10 mRNA were detectable.

结果：T细胞无能为抗原特异性的，多克隆激活剂PHA、CD3单抗和PMA+A23187可以恢复无能T细胞的活性；IL-2可以阻止T细胞无能的形成，但不能逆转其耐受状态；无能T细胞IL2和IFN mRNA是关闭的，而IL-4和IL-10 mRNA则是开放的。

After inactivated by mito C, the anergic CD4〓T-cell was added in medium of new isolated the same donor CD4〓 T-cell and the recipient CD4〓 T-cell respectively, the same experiments with the anergic CD〓T-tell and supernatants.

TJU103联合CTLA4-Ig应用，对供着T细胞特异性增殖和细胞毒活性的影响 TJU103联合CTLA4-Ig应用能够抑制供者T细胞增殖，A492nm值为0.032±0.004，且具有抗原特异性。

Wenxin capsule can promote endolethial cells releasing CO and synthesing 〓, enhance activity of HO, induce angiectasis through activating guanylic cyclase and adenglate cyclate of intracellar of vessel smooth muscle cell to enhance content of intracellar cGMP and AMP. 3. Wenxin capsule can inhibit releasing of platelet activiting factor 〓 which inhibit platelet aggregation and have antithrombosis function. 4. Wenxin capsule can promote activity of t-PA, reduce activity of PAI-1, strengthen fibrinolytic function, therefore inhibit fibrin disposition. 5. Wenxin capsule can inhibit synthesis of smooth muscle cellular DNA. Its function don't kill it directly but prevent transformation from period Go to peried S. 6. Wenxin capsule is an effective prescription of anti-coronary atherosclerosis atherosis. The results of image pattern analysis tastifies that area of lipid plaque in treating group reduce 54% and 58% in contrast with that in control group. The results of light, electro- microscopy show that structure of endolethial cell and smooth muscle cell in treating group is more integral than that in control group.

其作用机制之一是由于该药具有良好的调脂降脂作用，其不仅能明显降低血清LDL-c及升高HDL-c含量，且能有效阻止TC沉积于血管壁，从而保护内皮细胞结构完整；此外，温心胶囊尚具有明显的抗氧化作用，该药不仅能明显降低血浆LPO含量，且能提高SOD活性，同时其对血红素在细胞内代谢的调节作用也参与了抗氧化过程。2 温心胶囊能明显促进内皮细胞释放CO及合成〓，提高HO活性，通过激活血管平滑肌细胞内鸟苷酸环化酶及腺苷酸环化酶引起细胞内cGMP、AMP浓度增加而引起血管舒张。3 温心胶囊能明显抑制血小板激活因子〓的释放，从而抑制血小板聚集，抗血栓形成。4 温心胶囊能明显提高t-PA活性，降低PAI-1活性，增强纤溶功能，从而抑制纤维蛋白沉积。5 温心胶囊能明显抑制平滑肌细胞DNA合成，其抑制作用不是直接杀伤抑制，而是阻止细胞由Go期向S期转化。6 温心胶囊是抗冠状动脉粥样硬化的有效制剂，图象分析结果证实，该药治疗组脂斑面积比对照组缩小了54％和58％，光电镜结果显示内皮细胞及平滑肌细胞结构较为完整。

The resultsshowed that malignant mesothelioma may be new histologic subtypes, including signet ringoidcell, small cell myxoid, myofibroblastic cell, lymphohistiocytoid, angioblastic cell and undifferen-tiated types.

结果发现，恶性间皮瘤新近的几种亚型有印戒样细胞型、小细胞型、粘液样型、肌纤维母细胞型、淋巴组织细胞样型、血管母细胞型和未分化型。

Different measures of potentill anserine extract has different effects on endothelial cell of capillary vessel of small rats' alveolar wall. Endothelial cell of capillary vessel of alveolar wall has hyperplasia and hypertrophy in different degrees; it leads to permeability of capillary vessel increasing in different extent. With the situation, different concentration of potentill anserine extract promotes vascular endothelial cell factor of small rat's pulmonary tissue secretion.

不同剂量的蕨麻提取物对小鼠肺泡壁毛细血管内皮细胞的影响不同，肺泡壁毛细血管内皮细胞呈不同程度的增生肥大导致毛细血管通透性呈现不同程度的增加，表明可能和不同浓度的蕨麻提取物促进小鼠肺组织某些血管内皮细胞因子（Vascular endothelial cell factor VECF ）的分泌功能有关。

Grew on the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca(superscript 2+) localization mainly concentrated within the vacuoles and intercellular spaces and there was also some Ca(superscript 2+) deposits in cell walls. But when Garyota urens L. was treated by the temperature of 2℃ for 48 h, the level of Ca(superscript 2+) increased in cytoplasm and plasma membrane, but decreased in vacuoles and intercellular spaces considerably. At the same time, the ultrastructure of chloroplasts suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoids was exiguous and unclear, the photosynthetic rate decreased evidently. And when Garyota urens L. was treated by the temperature of 2℃ for 120 h, the deposits of Ca(superscript 2+) mainly concentrated within the cytoplasm, nucleus and plasma membrane and there was also some Ca(superscript 2+) deposits in vacuoles, and the ultrastructure of some cells was simultaneously damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear, and the cell only have respiration.

研究结果表明，未经低温处理的董棕幼苗叶肉细胞，焦锑酸钙沉淀颗粒大量出现在液泡和细胞间隙中，细胞壁中也可见少量沉淀，而细胞基质中则看不到焦锑酸钙沉淀；经2℃ 48 h低温处理后，细胞基质和细胞膜上焦锑酸钙沉淀增加，而液泡和细胞间隙中的焦锑酸钙沉淀则显著减少，并且超微结构已初步显示出寒害的特徵，叶绿体外膜部分破损，类囊体片层稀疏且排列不规则，光合速率明显下降等；经2℃ 120 h低温处理后，细胞间隙内的焦锑酸钙沉淀极少，有的也紧贴在细胞外壁上，而细胞基质和细胞膜上则分布有非常多的焦锑酸钙沉淀，在核基质和液泡中也可见到少量的焦锑酸钙沉淀，并且超微结构遭到了显著破坏，叶绿体结构完全被破坏，核膜与液泡膜严重破损，内部结构模糊，细胞只表现为呼吸作用，不进行光合作用。

The quantities of starch grains in the two"cells"in micropyle part of embryosac gradually decrease and become unclear.With the further development of embryosac the starch grains in the central cell and antipodal cell disappear in succession,however,the egg cell does not.

在准极核移向胚囊中部前后，不同的功能性细胞有不同的表现，珠孔端的两个&细胞&淀粉粒数量逐渐减少并变得不明显，随着胚囊的进一步发育，其中中央细胞和反足细胞内的淀粉粒相继消失，但卵细胞内却一直保存着。

The results show that the normal process begins with archesporial cell and undergoes stages of primary and secondary sporogenous cell,microspore mother cell,dyad,tetrad,central nucleus microspore,vacuolated microspore,mature microspore,twocell pollen and threecell mature pollen.

对平流层辐射处理SP3谷子和对照CK3谷子雄性细胞发育的研究表明，雄性细胞正常发育过程从孢原细胞开始，经初生造孢细胞、次生造孢细胞、小孢子母细胞、二分体、四分体和单核小孢子中央期、单核小孢子液泡期、单核小孢子成熟期直到二细胞花粉、三细胞成熟花粉结束。

The results show that the duration of the 3 phases during the culture of cell suspension is related to the inoculum size, the initial nutrient concentration, the feeding of nutrients and the culture conditions such as temperature and dissolved oxygen, that the maximum cell yield reaches about 12g/L for batch culture or 35g/L for fed-batch culture in dry weight, that the saponin content of cells quickly increases in the bloom phase and keeps on increasing until the early stage of the declining phase, with a maximum saponin content of about 60~70g/kg in dry weight, that the conductance value of cell suspension decreases from more than 8000μS to less than 600μS with the growth of cells, but increases when the cells autolyze, and that part of cells turns red in the later culture, which has no apparent effect on the saponin content.

结果显示：细胞3个生长时期的持续时间与接种量、初始营养浓度、营养流加情况及培养条件有关；分批培养和流加培养时的最大细胞得率分别为12g/L和35g/L；细胞的皂甙含量在旺盛生长期快速上升，并保持增长至衰退期前期；干细胞中最大的皂甙含量达60~70g/kg；随着细胞的生长，培养液的电导值从8000μS以上下降至600μS以下，细胞自溶后逐渐回升。另外，培养后期部分细胞颜色变红，但没有发现颜色转变对皂甙含量造成影响。

The use of the web to spread fears that flicking the switch on the LHC could create a Black Hole that could swallow up the Earth particularly concerned him, he said.

他说，使用互联网散布恐慌说启动LHC会生成一个可能吞噬地球的黑洞的做法让他感到忧虑。

Not a single word have I written down.

我一个字也没有写下来。