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Then identify the separated bacteria according to its shape feature and the physiological biochemical reaction results. As the result a strain of bacteria which could consume ethanol efficiently was selected and identified as Acetobacter.

在以乙醇为唯一碳源的培养基中培养，用梯度驯化法筛选高效利用乙醇的菌株，并通过形态特征和生理生化实验进行菌属鉴定。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

On July 21, a RMB's exchange system, characterized by an administrable floating rate and also basing on the supply and demand of markets as well as adjusting it according to a package currency, was put into practice by the China Center Bank.

7月21日，央行宣布开始实行以市场供求为基础、参考一揽子货币进行调节、有管理的浮动汇率制度。

The study was aided by the Ailuropoda Fund and designed on account of the Chengdu zoo' s topic that the Detection of the feline distemper' s serum antibody and the foundation of its immune programme about the grow wild feline animal such as Panthera pardus L, et al. The study regarded the attenuated feline panleukopenia virus as the diagnostic antigen which was isolated from the tri-combinant vaccine of Feline rhinotracheitis virus, calicivirus and panleukopenia virus . According to the tesults of Virus Physical and Chemical Character Detection, Agar Diffusion Reaction and Virion Shape Electron Microscope Observation, it was affirmed that the virion which was isolated from the tri-combinant vaccine was feline panleukopenia virus .

本研究从猫瘟热病毒、猫鼻气管炎病毒、猫鼻结膜炎病毒三联弱毒活疫苗中分离猫瘟热病毒弱毒株，通过对分离株进行病毒理化特性检测、双向琼脂扩散试验检测和病毒粒子形态电镜观察，最终确认从三联弱毒疫苗中分离到猫瘟热病毒，并以此分离株作为诊断抗原。

This paper, aiming at the weak points of the low early strength and the slow setting and hardening of portland fly-ash cement , according to the forming mechanism of cement clinker minerals, cement clinker minerals are choosen, fly ash is used as a substitute of clay as raw materials, early and high strength type cement clinker has heen developed, the amount of alit in the clinker is very high.

本文针对粉煤灰水泥早强低，凝结硬化慢等缺点，根据水泥熟料矿物的形成机理，用粉煤灰代替粘土作原料，对熟料矿物进行优化组合，成功地研制出了一种高硅酸三钙的早期强度高的水泥熟料，这种熟料可掺入30％一40％粉煤灰，生产出425R型粉煤灰水泥。

According the spatial correlation, it is believed that the Jintananshan Fault as well as the whole fault bundle is the eastward-extending part of the Altun Fault. The special modality of the fault bundle, which spreads out eastwards, is in favor of decomposing and absorbing the slip movement, which is normal at a strike-slip fault's ends.

分析认为金塔南山断裂以及整个阿拉善南缘断裂束是阿尔金断裂左旋运动的东延部分，断裂束在平面上&帚状&的、向东撒开的构造样式有利于走滑运动量的分解、消减和吸收，符合走滑断裂末端的构造特点。

Dean Kuehnen Jr. is suing his ex-fiance, Andria Castellano, to compel her to either return the ring or give him cash -- as well as cover his legal fees and costs, according to the complaint filed in New York State Court.

根据纽约州法院受理的投诉诉讼中称，Dean Kuehnen Jr要起诉他的前未婚夫Andria Castellano强迫她还订婚戒指或还钱，此外还有他的法律费用。

According to Gadamer,"on-being"was still a kind of transcendent existence, and he accepted"comprehension"as the subject of theory on interpretation, and regarded"possession antecedently, beholding antecedently, grasping antecedently"as the ontological structure of theory on interpretation.

在伽达默尔那里，此在仍然是先验性的存在，他把海德格尔所谓的生存结构的一种样式——领会作为解释学本体接受下来，把&先行具有、先行见到、先行把握&的解释学循环看作解释学的本体结构。

The government trimmed as many as 150,000 jobs in the 1990s, but since then has taken on twice that number, according to research at Banco Santander, the Spanish bank that is one of Brazil's biggest foreign investors.

西班牙国家银行提供的数据显示，虽然巴西政府上世纪90年代削减了多达15万个政府职位，但自那以来新增的政府职位却是这一数字的两倍。这家银行是巴西最大的外国投资者之一。

As for beamless slabs with the same amount of prestressed reinforcement and non-prestressed reinforcement but different reinforcing ways, the bearing capacities are considered to be the same according to former experiment results. Based on this point, the arrangement of tendons is considered to be arbitrary by equivalent frame method as long an the amount of tendon is unchangeable.

对于仅预应力筋布置方式不同，而配有相同数量的预应力筋及非预应力钢筋的无梁板，以往的试验结果和理论分析揭示了它们具有相同的极限承载能力，很多人依据这一结论，认为只要保证了无粘结预应力筋的总量，其布置方案可以是任意的，因此采用等代框架法计算、设计预应力筋。

They go to town. Would you just ask them?

他们在城里你能问他们吗？

Ossein pretreated for a2 weeks period exhibited the maximum% yield incase of bone gelatin, while the yield from intact skin was higher than that realized incase of de-scaled skin.

预处理时间达2星期的骨胶原表现出最高的骨明胶百分产量，然而从未处理鱼皮中得到的产率比去鳞的鱼皮得到的要高。