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The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

According to the wheel-axle production line project of Ji" nan locomotive and rolling stock plant, this dissertation put forward "Designing of wheel-axle foundation production line and management of wheel-axle information and mainly carried on following several aspect works: According to current new technique of the domestic and international wheel-axle assembling is researched, and combining present produce condition, we have designed the machining and assembling product line of axle and wheel and non axle box rolling bearing assembling product line.

根据济南机车车辆厂轮轴工艺线建设项目，本文提出了"货车轮轴基础工艺线设计及轮轴信息化管理"研究课题，并主要进行了以下几方面工作：通过对目前国内外轮轴加工组装的新技术新工艺进行研究，结合工厂轮轴生产现状，对货车轮轴生产线进行了工艺方案设计。

Particular configurations on bedding planes and on the surface perpendicular to the bedding planes lead to different explanations for the genesis of these silty dykes:(1) they are considered as the oldest trace fossils of metazoan according to the spindle-crack of siltstones on the bedding plane and the sand-filling tube configurations on the surface perpendicular to the bedding plane;(2) they are considered as the liquefied sand-veins induced by earthquake according to the sinuous configuration of siltstone dykes on the surface perpendicular to the bedding plane and the obvious liquation feature.

由于该粉砂岩岩墙在层面上和层内具有特别的形态，因而对其成因具有多种解释：在层面上呈纺锤状粉砂岩裂缝以及在层内呈砂质充填管形态，从而被认为是最古老的后生动物遗迹化石；在层内呈弯弯曲曲的粉砂岩岩墙以及明显的流体化作用特点，所以又被解释为地震振荡作用形成的液化砂岩脉。

According to Raz, the function of law is to rule people\'s actions, which helps to solve the problem of social cooperation and helps people behave properly according to the right reason.

在拉兹看来，法律的功能是规制人的行为，它有助于解决社会协作问题，并在社会协作中帮助人们按照正确的理由行为。

However, according to Chinese law, if the shareholders do not behave properly according to the law, they may be forced to take joint liabilities for the debts of the company under certain circumstances.

然而，根据有关中国法律，如果股东有某些违反法律规定的行为，他们对公司的债务应当承担连带责任。

The preoperation diameter of ascending aorta was taken as baseline. Patients were divided according to its baseline aortic diameter into diameter0 mm group and diameter≥40 mm group, furthermore, the patients were also divided according to echocardiographic findings into bicuspid aortic valve group and tricuspid aortic valve group.

以术前升主动脉直径为基础值，按基础值的大小分为直径﹤40mm组和直径≥40mm组，并按主动脉瓣形态分为二叶主动脉瓣组和三叶主动脉瓣组。

Is an ambulation, hold an equipments then according to the wireless correspondence system of blue tooth and fix terminal, can immediately carry out an auto each other to link in need not cable inside the short distance of an importance constitute part, the correspondence of of each equipments passes a blue tooth mold a piece to carry out;Each equipments because of oneself of pick up a people what characteristic and special structure limit according to the program design problem of these equipmentses, pass Java miniature, the version(J2 ME) resolves to some extent.

基于蓝牙的无线通信系统是移动、便携设备和固定终端，在短距离内不用线缆即可实现自动彼此连接的一个重要组成部分，各设备之间的通信通过蓝牙模块来实现；各设备由于本身的接口特性和特殊的构造所限制的基于这些设备的程序设计问题，通过Java微型版(J2ME)在一定程度上解决。

According to the original plan to plant gum ebastine oncontour des cription, yin to write off a bit of the solid line that the leucogranites part of the dashed line just like the Internet image dot propomon descend to steam-and-ink, the line displayed is referred to as "blue mold" in translucent Lithographing of adhesive tape, then to bandnoise and extracranium needle stroke, put on, and then charged with paste paper will be charged against print-out, transfer to a long fibers'interface scourability yarn of paper, to sweep the LAN Dian powder, and will be responsible for the falling rocks, flushed with water, it emerged, each color will have to follow the program falls, injuring a writing brush and steam riemerella Tsui pen-and-ink hand according to the profile, the tone of a dense gradient is vvibdv Tsui pen point network.

照原图以植物胶依边框勾描，晴位部以不实线表现，浅色局部以实线不离像上钩图像的深浅网不面比例，以汽火不朱不兵线条表现不入来，称为「蓝模」，刻画在半透明的石印胶纸上，再以针笔勾瘪纹，擦上黑不朱，再用浆糊纸不兵黑不朱不正印不入来，转印于一种不幼纤维的冲纱纸上，以蓝靛粉扫上，再不兵之功降石上，以火冲刷，边框便呈现不入来，每一个印色便给依步调降一块石，再以羊毫及鸭咀笔用汽火不朱依边框手描，浓不浓不突变色调则给用幼咀钢笔不面网。

In order to reduce the error of a remote sensing area sentencing in two-stage survey of investigations, this article used the Bo'ai County state-owned tree farm partial remote sensing TM satellite picture and the correlation data establishes the visual solution to symbolize that, according to the solution which established translates the symbol to the place thing to carry on the remote sensing to interpret,cancelled in the room draws each place kind of boundary, extracted on the chart with the polyester membrane the area, may calculate according to the proportion interprets the area ,figured out interprets the area, simultaneously in order to enhance interprets the precision to obtain a more real reliable material, investigated 90 bottom classes in kindergarten's area from the location, and interpreted to these 90 bottom classes in kindergarten on the chart obtains the corresponding area,simultaneously established the linear model.

为了减少二类调查中遥感地类面积判读误差，本文利用博爱县国有林场部分遥感TM卫星图片和相关资料建立了目视解标志，根据所建立的解译标志对地物进行遥感判读，在室内勾绘出各地类边界，用聚酯膜求出图上面积，根据比例计算出判读面积，同时为了提高判读精度得出更加真实可靠的资料，从现地中调查了90个小班的面积，并对这90个小班在图上判读得出相应的面积，同时建立线性模型。

DARBY: Paul, bondman of God, and apostle of Jesus Christ according to faith of God's elect, and knowledge of truth which according to piety

CUVMP：神的仆人，耶稣基督的使徒保罗，凭著神选民的信心与敬虔真理的知识

This time, we do not want to colorize the image. I've dragged my Hue slider to the left to a value of -123

这次不要勾选着色，我将色相的滑标向左移到-123

A timely report from an independent prosecutor would surely be helpful in buttressing and justifying that order.

及时报告从一个独立的检察官一定会有助於支撑和合理的秩序。