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If cells are not fixed with paraformaldehyde, keep the samples on ice and run them immediately after staining is accomplished

如果做好不能及时检测，我们一般是加入等量的2%的多聚甲醛固定，4度冰箱避光贮存。

If cell s are not fixed with paraformaldehyde, keep the samples on ice and run them immediately after staining is accomplished

如果做好不能及时检测，我们一般是加入等量的2%的多聚甲醛固定，4度冰箱避光贮存。

Secondly, the paper is expanded from follow three aspects: the virtual design, the virtual process and the product data managementduring the developing the torque converter. Applying the key technique of concurrent engineering---design for function, design for assemblyand design for manufactureof the method of design for "X", the virtual design of the torque converter is accomplished in CATIA V5. Then the model constitution and the simulation of virtual process of the torque converter is done in CATIA V5, including the model constitution and the simulation of 5-aixe NC virtual process of the vane, the model constitution（material:PVC） of the idler pulley rapid prototypingand the virtual assembly and the motion simulation of the torque converter, etc. 3-D data exchange between various CAD systems is realized by using STEP standard. The lever of classing the character model of the torque converter is given. And the access authorization is set up and given to manage the partners' reading/writing the product data.

应用并行工程的关键技术之一——面向产品全生命周期的设计DFX中面向功能的设计DFF、面向装配的设计DFA和面向制造的设计DFM的方法，在CATIA V5中完成液力变矩器虚拟设计；在CATIA V5中对液力变矩器进行虚拟加工建模和仿真，包括液力变矩器导轮叶片五轴数控加工刀轨建模与仿真、导轮快速成型RP建模、液力变矩器虚拟装配和运动仿真等；采用STEP标准实现多CAD系统之间三维数据交换，提出液力变矩器特征模型的分级标准并对三维模型数据进行分级管理，制定相应的权限等级以规范合作厂家的产品数据管理层次。

Design of the schematic、PCB layout and manufacture、measurement of the proposed image processor is accomplished.

完成了实时图像处理器的原理图设计、印制电路板设计和硬件的安装、调试。

Solvent extraction is accomplished by the addition of an immiscible solvent to the wastewater.

溶剂萃取是向废水中投加不溶于水的溶剂。

Methods From 1994 to 1999, a new endourethral stent has been implanted for 99 high risk patients with urinary retention caused by prostatic hyprtplasia. The implantation was accomplished with a new implanter under the guidance of intraoperative transrectal ultrasonography.

总结1994年2月应用新型支架、置入器械，改进置入技术，治疗99例高危前列腺增生症伴尿潴留患者的临床疗效。

Based on the segmented unstructured model of Nosiheptide fermentation process, the secondary variables are selected according to the implicit function existence theorem. The on-line identification of fermentation phases is accomplished by using an indicator variable which is gained by mathematical inference, and for each phase, a local soft sensor model is developed.

首先以分阶段的诺西肽发酵过程非结构模型为基础，根据隐函数存在定理进行辅助变量的合理选择；然后利用经数学推导得到的指示变量"伪比生长率"完成发酵阶段的在线辨识，并采用神经网络构建出对应于各阶段的局部软测量模型。

That the foundations laid down by the Commons, on the trial of Doctor Sacheverel, for justifying the revolution of 1688, are the very same laid down in Mr. Burke's Reflections; that is to say,—a breach of the original contract, implied and expressed in the constitution of this country, as a scheme of government fundamentally and inviolably fixed in King, Lords and Commons.—That the fundamental subversion of this antient constitution, by one of its parts, having been attempted, and in effect accomplished, justified the Revolution.

在审判Sacheverel博士的案件中，国会众院为1688年光荣变革辩护时所阐明的基础，与在伯克先生的《回应》中所阐明的完全一样；那就是说——撕毁这个最初的契约，在我们这个国家的宪法中隐含着或被明确表达的作为依附于国王，贵族院和众院的根本与不可更改的政府框架——对这份古老宪法的根本性的变更，它的一部分曾被试图修改并且实际上已经完成，这证明了光荣变革的正当性。

Accomplished thing of impracticable of a lot of enterprises.

做到了很多企业做不到的事情。

The mostly accomplished tasks are finally summarized in the paper, some improvable aspects are put forwards and further research to improve the system are presented.

论文最后总结了研究过程中取得的成果，分析了系统的不足，指出了进一步完善的方向和途径。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。