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Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

Taking mRNA from ephebic Bombyx mandarina as templates,the cDNA of four troponin I isoforms was cloned by RT-PCR,named TnI-A,TnI-B,TnI-C and TnI-D(GenBank accession numbers:FJ785828,FJ785829,F3785830 and FJ890515 )respectively.Sequence homology comparison and functional prediction of the amino acids encoded by the four TnI gene isoforms in Bombyx mandarina indicate that they have characteristics of troponin families.

与电子克隆到的相应的家蚕TnI亚型比较分析，结果显示：野桑蚕与家蚕TnI-B有相同的氨基酸，TnI-A和TnI-C有一个氨基酸的差别，而在家蚕中没有电子克隆到与野桑蚕TnI-D相同的亚型。3。

A mesophilic eubacterium, among all fully sequenced prokaryotic genomes up to date. Computational analysis on genes involved in basic metabolic pathways supports the experimental discovery that T.

结果显示，基因组大小为2，689，445-bp（Genbank Accession NumberAE008691），包含2，588个编码序列（coding sequences，CDS），其中1，764个CDS（68.2％）与已知蛋白质的序列同源，其余824个CDS（31.8％）的功能未知。

Thermoanaerobacter tengcongensis is a rod-shaped, Gram-negative, anaerobic eubacterium that was isolated from a fresh water hot spring in Tengchong, China. Using a"whole-genome-shotgun"method, we sequenced its 2, 689, 445-bp genome from an isolate, MB4〓(Genbank Accession Number AE008691). The genome encodes 2, 588 predicted coding sequences . Among them, 1, 764 (68. 2％) are classified according to homology to other documented proteins, and the rest, 824 CDS (31. 8％), are functionally unknown. One of the interesting features of the T. tengcongensis genome is that 86. 7％ of its genes are encoded on the leading strand of DNA replication.

中文题名腾冲嗜热厌氧菌全基因组测序及分析副题名外文题名 A complete sequence of the T.tengcongensis genome 论文作者包其郁导师杨焕明研究员学科专业遗传学研究领域\研究方向学位级别博士学位授予单位中国科学院遗传与发育生物学研究所学位授予日期2002 论文页码总数88页关键词腾冲嗜热厌氧菌基因组嗜热菌馆藏号BSLW /2003 /Q939.1 /33 腾冲嗜热厌氧菌（MB4＇）是分离于我国腾冲地区温泉内的革兰氏阴性厌氧真细菌。

Results Beta-tubulin genes, 1439bp and coding for 443 amino acids, was acquired and was submitted to GenBank and to get an accession number that was AY220457. The beta-tubulin gene of S. japonicum and Fasciola hepatica were homologous with an identity of 79%.

结果 获得了1个日本血吸虫新基因－微管蛋白基因，全长1439bp，编码443个氨基酸，与肝片形吸虫微管蛋白基因具有79%的同源性。

Within the Central European part of Russia's ethnic turn of the century the rise of the core area of .15 in Moscow as the center to form a centralized state in Moscow Duchy .1547 expansionist years later, vast tracts of land in neighboring countries Gezhan .1917 11 7 On the establishment of the Russian Soviet Federative Socialist Republic, in December 1922 with Ukraine, Belarus, South Caucasus Federation composed of the USSR (Union of Soviet Socialist Republics). 1924 -1940, Lithuania and other accession, a total of 15 former republics.

境内欧洲部分中部是俄罗斯民族兴起的核心地区。15世纪末形成以莫斯科为中心的中央集权国家莫斯科公国。1547年后推行扩张主义，割占了邻国大片土地。1917年11月7日成立俄罗斯苏维埃联邦社会主义共和国，1922年12月同乌克兰、白俄罗斯、南高加索联邦等组成苏联（苏维埃社会主义共和国联盟）。1924年—1940年，立陶宛等加入，共15个加盟共和国。

Each illegal sector has its own reasons for success: internet banks are easier to rob than high-street ones, for instance, and passport forgery has become easier since the accession to the EU of countries with more easily forgeable documents.

每个非法领域都有其自身成功门道：比如说，网上银行比位于主干街道上的银行更容易抢劫，及，自从进入欧盟国家的文件伪造起来简单得多，伪造护照也随之容易。

This study not only implicated a possible accession for the management of diseases characerised by pathological cell proliferation using drugs targeting PI3K/PTEN/PKB signal system, but also afforded theory evidence for the ideal results of the two agents in the prevention and treatment of atherosclerosis and restenosis after interventive myocardial reperfusion.

此项研究不仅为研制PI3K/PTEN/PKB调节药物用于以细胞过度增生为主要病理改变的疾病治疗展示了可能途径，更为该两种药物在冠心病一、二级预防或冠脉介入治疗后再狭窄防治中取得的理想疗效提供了理论支持。

And we will supply more products for customer with the accession of types of Shanghai NIHON KOHEN.

由于上海光电厂的产品品种不断增多，因此相信我们为客户所提供的产品也会不断增多。

The sequences of 16SrRNA, lipA gene and the deduced amino sequence had been disposited in. GenBank, with the accession number EU439251, EU414288 and ABY86751 respectively.

Pseudomonas sp.lip35的16SrDNA序列已在GenBank中注册，注册序列号为：EU439251；lipA基因序列及由其翻译得到的氨基酸序列已在GenBank注册，登录号分别为EU414288，ABY86751。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。