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Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Law culture surface layer structure, as the expression of the laws culture deep structure, under the condition of the China s accession to the WTO, especially international globalization, alters fast and changes into the trends of the modern accession and becomes an important non-slighting nacreous section of the culture in the world laws culture system.

内容摘要：法律文化的表层结构，实质上作为法律文化深层结构的法律意识形态的外在化表现形态，在中国入世的条件下，尤其是在世界经济全球化的浪潮中，迅速转型和改变，演绎成为现代化进程的趋势，成为多元并存的世界法律文化体系中不可忽视的、具有光彩的一只重要文化。

Sequence analysis showed that the full-length cDNA sequence of Sc-SAMDC (GenBank Accession number: GQ246459) was 1 968 bp, with three open reading frames, tiny ORF, upstream ORF and main ORF. The mORF was 1200 bp encoding 399 amino acid residues with a molecular mass of 43.6 kD, and the deduced protein had two highly conserved function domains (proenzyme cleavage site and PEST domain).

序列分析显示，甘蔗Sc-SAMDC基因(GenBank Accession number： GQ246459) cDNA全长1968 bp，存在3个读码框（袖珍读码框tORF、上游读码框uORF和主读码框mORF），mORF长1200 bp，编码399个氨基酸的SAMDC酶原，预测分子量为43.6 kD，该酶原含有两个高度保守的功能结构域（酶原剪切位点和PEST结构域）。

The 6 AChE Genes from L.bostrychophila,L.entomophila and L.decolor were demarcated into Insect TypeⅠAChE Gene and Insect TypeⅡAChE Gene.4 Gene cloning of nAChR and mRNA expression level in L.bostrychophilaTwo nicotinic acetylcholine receptor subunit genes,Lb al and Lb a8,were cloned from the psocid L.bostrychophila.The full length cDNAs of Lb a1(GenBank Accession No.: EU871527) and Lb a8GenBank Accession No.

昆虫的两类AChE严格的区分，其中Ⅰ型AChE先与脊椎动物AChE聚合后才与Ⅱ型AChE相聚，说明两类AChE基因在物种分化前就已经分化。4嗜卷书虱烟碱型乙酰胆碱受体基因克隆及其mRNA表达水平研究利用RT-PCR和RACE技术成功克隆获得了嗜卷书虱2个烟碱型乙酰胆碱受体亚基的全长序列，分别命名为Lb a1(GenBank登录号：EU871527)和Lb a8(GenBank登录号：EU871526)。

Based on the cloning of Spidroin2 cDNA of Nephila clavipes (Genbank Accession No. AF441245), we constructed a prokaryotic expression vector pET-28b-Sp by double digestion.

本研究在获得生长于中国的Nephila clavipes蜘蛛牵引丝蛋白Spidroin2 cDNA（Genbank Accession No。

Bioinformatics analysis showed that the gene sequence was highly consistent with previously reported F3,5H genes, such as GenBank Accession D14588, Z22545, and DQ352142. The encoded amino acid sequence was highly homology with that of GenBank Accession BAA03438.1, BAC10997.1 (Nierembergia sp. NB17) and AV85470.1 at 99%, 88% and 87%, respectively.

生物信息学分析表明克隆的F3，5H基因与GenBank登记号D14588、Z22545和DQ352142等的F3，5H基因序列高度一致；其编码的氨基酸序列与GenBank登记号BAA03438.1、BAC10997.1和AAV85470.1的高度同源，同源率分别为99%、88%和87%。

In contrast,the expression levels of Lb est2 in nymphal stages are lower than that in adult stage.2 Gene cloning of AChE and mRNA expression level in L.entomophila2.1 Gene cloning and sequence analysis of AChE in L.entomophilaTwo full length cDNA encoding AChE were cloned from L.entomophila by the methods of RT-PCR and RACE,named Le ace1(GenBank Accession No.:EU854149) and Le ace2GenBank Accession No.

CarE基因在不同发育阶段mRNA表达量的分析结果表明，Lb est1的表达量随着该虫生长发育的进行逐渐降低，至成虫期时表达水平最低；相反，L6 est2在若虫期表达水平较低，而在成虫期表达水平最高。2嗜虫书虱乙酰胆碱酯酶基因克隆及其mRNA表达水平研究2.1嗜虫书虱乙酰胆碱酯酶基因克隆及序列分析利用RT-PCR和RACE技术成功克隆获得嗜虫书虱2个AChE基在的全长序列，分别命名为Le ace1(GenBank登录号：EU854149)和Le ace2(GenBank登录号：EU854150)。

The genetic similarity coefficient analyses The genetic similarity coefficient between 85 accessions and their original backgroud Stylosanthes distributed between 0.6598 and 0.8351, in which No.3 accession was minimum, and No.85 accession was maximum.

在田间表现较好的7个株系中，2号与86号的相似系数最小，为0.6960，遗传距离最远；综合田间观察和AFLP分析，2号株系表现最佳，有望成为替代热研2号柱花草的新品种。

Noting China's intention to become a Party to the GPA, some members of the Working Party stated that China should, upon accession, become an observer to the GPA, and should initiate negotiations for membership in the Agreement by tabling an Appendix 1 offer within two years of accession.

注意到中国有意成为GPA参加方，一些工作组成员表示，中国应自加入时起成为GPA观察员，并应在加入后2年内通过提交附录1出价，开始加入该协定的谈判。

The sequence （GenBank accession DQ77520） contained a novel promoter region with TATA box,CAAT box and CA2 box-like elements in the identical positions common to the eucaryote promoter sequence regions in comparison with the sequences in the GenBank.

对该产物测序并提交GenBank数据库（GenBank accession DQ677520），经序列比对分析，发现该序列具有真核启动子序列的基本结构特征，含有TATA盒、CAAT盒、GC盒等元件。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。