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［Objective］To investigate the relation between Actinobacillus actinomycetemcomitans and human buccal mucosa epithelial cells in order to understand the invasion ability of A.a to epithelia.

［目的］探讨伴放线放线杆菌( Actinobacillus actinomycetemcomitans，A.a)与人口腔颊黏膜上皮细胞之间的关系，加深对 A.a侵入颊黏膜上皮细胞能力的了解。

Dendrogram divided 37 haplotypes into two major clusters, capra aegagrus clustered into lineage A, while capra falconeri clustered into other lineage. Lineage A is the predominance lineage of the 7 goat populations, while lineage B has a relatively small proportion.

系统发育树将37种单倍型划分为两个较大的分支，角猾羊1条序列聚入A分支，而捻角山羊则自成1支。A分支为7个群体的优势类型，B分支的影响相对较小。

The copper atom is in a planar coordination site of [N_2O_2] and it devites from the mean plane by only 0.80 pm. Byπ-πstacking interactions, a alabastrine structure was obtained.(2)Schiff-base complex [Cu(H_2O)]·H_2O, where H3GS is the 3-carboxyl -salicylidene glycine, was synthesized and characterized by elemental analysis, IR spectra and single-crystal analysis. The crystal belongs to monoclinic system, space group P2(1)/c, a=848.46(3)pm, b=681.54(3)pm, c=1967.16(8)pm,β=95.8210(10)°, Z=4, R_1=0.0279, wR_2=0.0724. The copper atom is in a square-pyramidal field with the base

结果表明该晶系属单斜晶系，空间群P2(1)/c，晶胞参数：a=848.46(3)pm，b=681.54(3)pm，c=1967.16(8)pm，α=90°，β=95.8210(10)°，γ=90°，Z=4，R_1=0.0279，wR_2=0.0724，Cu原子位于轻微变形的四方锥场底心，底面被氮原子、酚氧原子、甘氨酸羧基的一个氧原子和一个水分子氧原子占据，而甘氨酸羧基的另一个氧原子占据相邻分子的锥顶，因而形成一维链状结构；合成了单核双聚配合物Na_2[Cu_2_2]·2H_2O ，铜三核配合物Cu_3_2·5H_2O和铜锌异三核配合物ZnCu_2_2·5H_2O，并用元素分析，IR光谱，电子光谱和磁化率测定对配合物的组

To further study the chemical constitutes of lipophilic active parts, we prepared Alc95% and Alc50% according to original literature. 20 compounds were isolated by using macroporous resin, silica gel, polyamide, Sephadex LH-20, Rp-18, preparative TLC and preparative HPLC chromato-graphy. The structures of them were elucidated by means of physical and chemical properties, modern spectroscopic analysis techniques—UV、IR、 EI/MS、ESI/MS、〓H-NMR、〓C-NMR、DEPT、HMQC、HMBC. They are nine anthraquinones: chrysophanol (95-1), physcion (95-2), emodin (95-5), aloemodin (95-7), rhein (50-1), sennoside A or B (50-6),ω-hydroxylemodin (95-10), chrysophanol-8-0-β-D-glucoside (95-12), aloemodin-ω-0-β-D-glucoside (95-13); three phenanthaquinones: tanshinone ⅡA (95-3), cryptotanshinone (95-6), tanshinone Ⅰ(95-4); one isoflavone: 7, 3'-dihydroxyl-5' methoxyisoflavone (95-14) and protocaechuic aldehyde (95-9), protocaechuic acid (95-11), astragaloside Ⅳ(50-4), amygdalin (50-5), 2, 3, 5, 4'-tetrahydroxylstilbene-2-0-β-D-glucoside (50-3),-catechin (50-2), cinnamic acid (95-8). Among them, 3'-dihydroxyl-5' methoxyisoflavone (95-14) is a new compound.

经过溶剂萃取、大孔吸附树脂富集、Sephadex LH-20精制、制备TLC及Semi-pre HPLC等手段从中分离了20个化合物，利用理化性质和波谱技术(UV、IR、EI／MS、ESI／MS、〓H-NMR、〓C-NMR、DEPT、HMQC、HMBC)相结合鉴定了它们的结构，分别为9种蒽醌类化合物：大黄酚，大黄素甲醚，大黄素，芦荟大黄素，大黄酸，番泻苷A或B，ω-羟基大黄素(ω-hydroxylemodin)，大黄酚-8-0-β-D-葡萄糖苷(chrysophanol-8-0-β-D-glucoside)，芦荟大黄素-ω-0-β-D-葡萄糖苷(aloemodin-ω-0-β-D-glucoside)；3种菲醌类化合物：丹参酮ⅡA(tanshinoneⅡA)，隐丹参酮，丹参酮Ⅰ(tanshinone Ⅰ)；1种异黄酮：7，3'-二羟基-5'-甲氧基异黄酮（7，3'-dihydroxy-5' methoxyisofla-vone）以及原儿茶醛，原儿茶酸，黄芪甲苷(astragaloside Ⅳ)，苦杏仁苷，2，3，5，4'-四羟基二苯乙烯-2-0-β-D-葡萄糖苷（2，3，5，4'-tetrahydroxylstilbene-2-O-β-D-g1ucoside），Z—儿茶素，桂皮酸。

It may have a preventional function from Alzheimer's disease. Molecular biological studies are shown that apoE isoform has a isoform-specific role to neurodegeneration. ApoE〓 and apoE〓 aid to sustain the stabilization of cytoskeleton protein tau, while apoE〓 hyperphosphorylates tau to destabilize the microtubule protein, which will make neurocyte easy to form neurofibrillary tangles in AD. Clearance of amyloidal peptide by apoE〓 and apoE〓 is more effective than by apoE〓. ApoE〓 has the strongest ability to clear the amyloidal peptide. It is shown that apoE〓 has a stronger prevention from Alzheimer's disease.

分子生物学研究发现，apoE异构体对神经细胞退化表现出异构体特异作用，apoE〓和apoE〓有助于维持微管的稳定性，而apoE〓则使tau蛋白超磷酸化，破坏微管的稳定性，使神经细胞易于形成神经纤维缠结。apoE〓和apoE〓对A β的清除率远远高于apoE〓，apoE〓对A β的清除能力最强，可见apoE〓对AD的发生具有较强的阻止作用。

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化，结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受；和钙调磷酸酶相比，2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的，钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力；Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的，钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析，发现它是一个在哺乳动物中保守的，但功能未知的基因。

Full-term pregnancy of primipara 120 cases of illness; Stochastic double divides into three groups: research of group-A group and B group. control group of C group, A group (n=40), 0.75% levobupivacaine 10mg for CSEA, B group (n=40), 3% chloroprocaine the hard membrane pericoel hinders, C group: 0.75% bupivacaine for under the arachnoidea the cavity hinders the anaesthesia, In surgery process, to A and B groups, 2% lidocaine were intermittently injected respective-ly by epidural cathete, effec not enough of anesthesia.

足月初产妇120例随机双盲分成三组：研究组为A组和B组，对照组为c组。A组(n=40)蛛网膜下腔阻滞麻醉用药为0.75%左旋布比卡因10mg，使用腰麻硬膜外联合阻滞行剖宫产术，B组(n=40)以3％氯普鲁卡因为硬膜外麻醉的局麻药，使用硬膜外麻醉阻滞行剖宫产术，c组(n=40)腰麻用药为0.75%布比卡因10mg，使用腰麻硬膜外联合阻滞行剖宫产术。

3To introduce a minimally-invasive technique of a new percutanous external spinal fixation assistanted minimally-invasive surgical procedure treating thoralumbar fracture and analyse its clinic applicable area and value. [1]According to advantage of spine external transpedicular fixation technique and limitation of traditional percutanous external transpedicular fixation,design a new percutanous external transpedicular fixation.

(1)根据脊柱外固定技术的优点，传统脊柱外固定器的不足自行研制一套新型脊柱外固定器(WS-1型)；(2)取7具新鲜小牛腰椎标本，自制实验不稳定性骨折模型，把实验分成正常A组、骨折模型B组、新型脊柱外固定器固定C组、新型脊柱外固定器固定联合PVP技术D组，分别按顺序测试四组的三维稳定性，并测试A组、C组与D组在300N轴向压缩实验，再行统计学分析比较；(3)应用新型脊柱外固定器联合椎间盘镜微创椎管减压技术和PVP技术手术治疗胸腰椎骨折，病例选择：本组病例共69例，年龄自10岁-70岁，平均年龄56.3岁。

Bandi,the pirate with a parrot on his shoulder,found a treasure map but he soon lost it.A schoolboy called Peter picked up the map.he knew it was a treasure map,but he didnot know where he could find the treasure.

班迪，海盗与鹦鹉他的肩膀上，发现了宝藏地图，但他很快就失去了所谓的it.A小学生彼得拿起map.he知道这是一个宝藏图， 255 ，但他知道他在哪里可以找到treasure。

A boiler water controlling system with basifier a mg/L Na3PO4+b mg/L NH3+c mg/L NaOH is investigate in the paper. Several calculate methods are discussed and the precision pH value calculating equation is proposed for a cycle chemistry system that has seven changing parameters coexisting together, i. e. a mg/L Na3PO4+b mg/L NH3+c mg/L NaOH+d mg/L H2SO4+e mg/L CH3COOH+f mg/L CO2+g mg/L SiO2. As an example of equilibrium phosphate treatment, the pH value are obtained and showed in graphics with the four kinds of impurity changing from 0 to 1.0 mg/L. The calculating results can be used in practical boiler water pH value control.

对于一个采用a mg/L Na3PO4+b mg/L NH3+c mg/L NaoH碱化剂的炉水控制体系，主要讨论在炉水中存在d mg/L H2SO4+e mg/L CH3COOH+f mg/L CO2+g mg/ L SiO2 4种杂质时炉水pH值的计算方法，得到通用的pH值计算表达式；并以炉水平衡磷酸盐处理工况为例，计算这4种杂质在0-1 mg/L变化时，其单独或联合作用对炉水pH值的具体影响程度，以6幅图形展示计算结果，该结果可应用于炉水pH值的实际控制。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。