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Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

While this book's assessment of the future of our species was gloomy, Gray seemed to be making the point that, just as we emerged from the cauldron of evolution as a remarkable accident, an improbability, so too was our demise accidental, the result of overpopulation and overconsumption that was in turn the result of a series of extraordinary adaptations (the inventions of catastrophic agriculture and what we call civilization) necessitated by a horrifically bad roll of the cosmic dice (the striking of Earth by a meteor that wobbled its orbit and caused the ice ages).

然而，这本书对于我们物种的未来的评价是消极的，格雷想要表达的是：就如我们在进化的整个过程中，像是个伟大的意外，一种不太可能的情况下，出现了人类，我们的下场也是如此偶然，在天地宇宙间，偶然的一次坏运气（流行撞上地球，摇动了它的轨道，从而导致了冰河时代），使得人口过剩和消费过量与一系列不同凡响的改变（工业的发展带了灾难，我们却称之为文明）互为因果。

S Broad asphalt along the main road, followed by the array of more than 30 of its significant interest in large, medium and small Recreation: If the crazy "roller coaster" in the air on the track and came to a series of somersaults, and let you experience a soul-stirring centrifugal, rapid rotation of panic, can be done to stimulate;"fast scooters" in the track km On the fast, as Shirupozhu dive 30 meters high slope, Huer mad to Jixuan, feelings like Cloud Nine fall, as if riding a bull at running back; in particular, the addition of new large-scale projects,"Challenger Tour" Mars will give you the speed of excitement around the world,"wind speed," you over 10 meters in height, during which around and up and down several times before and after the turnover, there is a non-feet under the ultimate experience weightlessness; and the "monster House "is another boost fan, pitch dark, cold wind gusts, desperate calls and setting of zombie ghosts and volatile side of the creeps you scared, scared; 50 meters out to the" Ferris wheel "to let you enjoy the" List Small hills,"the sense of endless;" Liu Yong Jin,"When you enjoy the" drift "Huiguoshenlai no sense, as water spilled from the mountain 10 meters high fly drift down the moment, the heart Has been hanging in the air to the; also hit eyeful of Venus "bumper car"; as the mischievous people Shuaixiang half-empty,"Spider-Star,""rotating aircraft" and "forest mouse car," and so on, you will be able to bring a Although the stimulus was accompanied by tears, but more interest is the doubling of the situation, a test of your willpower.

沿着宽阔的柏油大马路，依次排列着三十多个各显其趣的大、中、小型游乐项目：如疯狂&过山车&在凌空的轨道上急转直下而又接连翻腾，让你经历一场惊心动魄的离心、快速旋转的恐慌，可谓极尽刺激；&快速滑行车&在千米的轨道上风驰电掣，势如破竹般俯冲三十米高坡，忽儿狂转急旋，那感觉仿如九霄云外下坠，又好象骑在蛮牛背上狂奔；特别是新增设的大型项目&挑战者之旅&将带给您火星绕地球的速度快感，&极速风车&，你凌驾十米高空中，其间几度上下左右和前后翻转，真有一种双脚无着下的终极失重体验；而&妖怪屋&又是另番刺激，伸手不见五指，寒风阵阵，绝望的叫声和出没无常的僵尸鬼面，吓得你毛骨悚然，胆战心惊；拔地五十米的&摩天轮&让你享受&一览众山小&的无垠感觉；&激流勇进&，当你优悠的&漂&感还没回过神来的时候，高山泻流水般从十米高处向下飞漂的那一刻，心已悬到半空中了；还有撞到满眼金星的&碰碰车&；恶作剧般把人甩向半空的&星际蜘蛛&、&旋转飞机&和&森林老鼠车&等，都能将你带到一个虽然伴有被刺激后的泪花，但更多的是兴致倍增的境地，考验了你的意志力。

Furthermore, the one-dimensional complexes 26 and 28 with strongπ-πpacking interactions are prepared from DMF solvent, which indicates that the traditional solvent volatilizing method could bring low-dimensional complex.4. By measuring the enthalpy changes of the liquid phase formation reaction for complexes a~e, and calculating the electronic densities of five 5-substituted derivation of isophthalic acid, we can make a conclusion that 5-substituted group changing of the ligand has a little influence on oxygen atoms of carboxyl groups.5. Fluorescence experiments of these complexes indicated that they could be used for luminescence materials in future.6. Using packing saturation rule we calculated and analyzed these series of complexes, and the results showed that the changes of complexes with changes of ligands could be explained from the rule.

在DMF溶剂中得到了具有强π-π堆积作用力的一维配位聚合物[Ln(5-NO_2-bdc)(NO_3)]Ln=Pr(26和Ho(28)，表明传统的溶剂挥发合成条件可以得到低维的配合物。4、通过配合物a～e的液相生成反应焓的测定，以及配体阳离子的电子密度计算，进一步佐证了间苯二甲酸的5位取代基变化对配体的羧基氧原子配位能力影响不大的结论。5、荧光特性表明此类配合物可以用于发光材料。6、利用堆积饱和规律对所得系列的配合物的进行计算和分析，发现随着配体的变化，配合物的变化可以从堆积饱和规律出发进行解释。

Jami Miscik, head of global sovereign risk at Lehman Brothers, points to a series of less dramatic events, which highlight the political strains caused by rising food and energy prices: riots in Mexico last summer, after sharp increases in the price of maize flour; mass protests in Indonesia this month, provoked by the rising price of soyabeans; a deadly stampede in western China last November, caused by a rush for subsidised cooking oil; a food-price freeze in Russia, introduced just ahead of the parliamentary elections in December; gas and petrol rationing in Iran; blackouts in Argentina and South Africa.

雅米尔·米希克是雷曼兄弟全球主权风险主管，他谈到了一系列不那么引人注目的事件，突显出食品和能源价格不断上涨所带来的政治压力。他谈到，在去年夏季，由于玉米面价格暴涨，墨西哥发生了暴动；因黄豆价格不断上涨，印尼本月爆发了大规模抗议活动；去年11月，由于抢购降价烹调油，中国重庆发生踩踏事件，致人死亡；就在去年12月杜马选举前夕，俄罗斯采取了冻结食品价格的措施；伊朗实行天然气和汽油定量配给制；阿根廷和南非则出现了停电事件。

I was told once that few % of world's population has access to the internet, so this "multimedial revolution" isn't something as big as we are told - by media - to think it is have the access to hardware, software and the now-how to create audio and video- artefacts on their own, at their homes. Every one of them has a possibility to try and use his/her personal computer make a sound or create a series of moving pictures, a possibility to express oneself other way than through hard work, family life or contestation of the status quo.

曾经有人跟我说这世界上只有极小百分比的人与网际网路相连结，所以多媒介的影响不如媒体宣染来的夸张，简单的说，只是现下有较多的人运用个人电脑在家创作一些声音或流动的影像，舒发工作压力，话家常，说说生活或对事物的论点吧。

Work on this weapon has been under way since the 1990s but it has been crippled by a lack of funding and a SAAF requirement that has not always been clear. The A-Darter will still notionally equip SAAF Gripens, but a long-awaited series of initial flight tests have yet to occur and the future of the programme is in doubt.

这种导弹的研制工作从上世纪90年代就开始秘密进行了，但由于缺少资金以及南非空军变幻不定的需求而步履蹒跚。A-标枪手理论上将装备南非空军的鹰狮战斗机，但是被期待已久的首飞测试仍然是遥遥无期，整个项目的未来仍充满变数。

Some of his best and least-known work resides in the collection "Cabbages and Kings", a series of stories which each explore some individual aspect of life in a paralytically sleepy South American town while each advancing some aspect of the larger plot and relating back one to another in a complex structure which slowly explicates its own background even as it painstakingly erects a town which is one of the most detailed literary creations of the period.

他某些最好和最闻名的收集工作寓于&大白菜、天王&，这一连串的故事，每一个探索了一些个人生活方面的城市，而南美paralytically困倦一些规模较大的进步每个环节一回又一阴谋，并与在一个复杂结构徐徐阐述自己的背景，甚至为它刻意一座城市是一个最详尽的文学创作该期

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

A more elastic footstep entered next; and now I opened my mouth for a 'good-morning,' but closed it again, the salutation unachieved; for Hareton Earnshaw was performing his orison SOTTO VOCE, in a series of curses directed against every object he touched, while he rummaged a corner for a spade or shovel to dig through the drifts.

接着，更有活力的脚步走了进来，而这次，我也开口道&早安&，但是没有人回应，我只好闭嘴。他在墙角的一堆杂物中找挖地用的铲子或铁锹的时候，开始轻声的进行他的祷告，其实是一连串的诅咒，诅咒每一件他碰到的东西。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。