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TheMADS box locates just at the 5'end of most of genes and is highly conserved.According to these,a primer was designed and the effort to isolate new membersof MADS box gene family in rice genome by RT-PCR was made.

绝大多数已克隆到的这类基因的MADS-box恰好位于5'端，而且非常保守，因此，我们根据MADS box基因的保守区设计了一个特异的引物，试图通过RT-PCR分离出水稻花中MADS box基因家族的新成员。

The Lyb genes were novel members of Y-box gene family containing a conserved cold-shock domain with two RNA binding motifs (RNP-1 and RNP-2). Moreover, phylogenetic analysis revealed that there was only one type of Y-box gene before the emergence of gnathostomata. By contrast, the Y-box gene were divided into three groups, YB1, YB2, and CSDA during evolution of jawed vertebrates.

此外， 从Swiss-Prot下载了经实验验证过的Y-box蛋白序列数据，结合实验克隆得到的Lyb基因所编码的氨基酸序列构建系统发育树，发现Y-box基因在有颌类出现以前只有一种类型，有颌类出现以后， Y-box基因分化成YB1、YB2和CSDA三种类型。

ARC1(arm repeat containing 1 ) is a very important downstream protein factor in sporophytic self- incompatible signaling process. It is made up of arm repeat domain, U-box domain, leucine zipper domain/ coiled-coil domain, one nuclear localization signal and two nuclear export signal, arm repeat domain and U-box domain are main functional domain.

臂重复蛋白ARC1(arm repeat containing1，ARC1)是孢子体型自交不亲和信号传导途径下游非常重要的蛋白质因子，由臂重复结构域、U-box结构域、亮氨酸拉链/卷曲螺旋结构域、1个核定位信号和2个核输出信号组成，其中臂重复结构域和U-box结构域起主要功能。

One of these clones,M79 was selected for further study.M79encodes a typical MADS box protein,and it shares high homology with many ofthe MADS box genes in plants.

对其中一个克隆M79的DNA序列和蛋白质序列分析表明，M79基因编码多肽含有一个典型的MADS box和K-box，与植物中的许多MADS box基因产物有很高的同源性。

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The main products are: all kinds of out of style, power distribution cabinets, as well as fixed capacitance cabinet; central air-conditioning automatic control system; a variety of lighting boxes, wall-mounted box, outdoor waterproof box, meter boxes, power boxes; a variety of fire control box , joint boxes, console; a variety of dual-power switch box, since the lotus root decompression starter boxes, variable frequency constant pressure water supply system.

主要生产的产品有：各种抽出式、固定式配电柜以及电容柜；中央空调自动控制系统；各种照明箱、挂墙箱、室外防水箱、电表箱、动力箱；各种消防控制箱、联动箱、控制台；各种双电源切换箱、自藕减压起动箱、变频恒压供水系统。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

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电气工程验收标准：漏电开关安装正确、使用正常；电气器件、设备的安装固定应牢固、平整；电器通电试验、灯具试亮及灯具控制性能良好；开关、插座、终端盒等器件外观完好，绝缘器件无裂纹，安装牢固、平正，安装方式符合规定；并列安装的开关、插座终端盒的偏差，暗装开关、插座、终端盒的面板、盒周边的间隙符合规定；弱电系统功能权齐全，满足使用要求，器具安装牢固、平正。

Was founded in 2000, the company is located in Des Pinghu Town, Longgang District, Shenzhen City Pass First Industrial Zone, Zhejiang Hongfeng plastics factory is in Shenzhen, branch offices, is a professional manufacturer of all kinds metal badges / lock key chain / tie clip / brooch / earring / medals / medals / medals / coins / necklaces fashion jewelry plastic jewelry box manufacturers, product reunification PS plastic injection raw materials from the product site for high - under a transparent cover for the black, the outer surface of the box according to customer demand a variety of pattern and gilt text box can be produced in accordance with the requirements of customers into various shapes lamination PVC Blister Flocking Flocking cloth and cloth, widely used in a variety of metal gifts and fashion jewelry, artificial jewelry industry first, and for chocolate, food packaging, we have developed a series of plastic box the size of specifications, the product a high degree of transparency, exquisite appearance and reasonable price, prompt delivery, quality reliable.

公司简介依美塑胶五金制品有限公司成立于2000年，公司座落于深圳市龙岗区平湖镇辅城坳第一工业区，属浙江鸿峰塑胶厂驻深圳的分公司，是一家专业生产各种金属徽章/锁匙扣/领带夹/胸针/耳环/奖章/奖牌/纪念章/纪念币/项链等时尚饰品用塑胶饰品盒的厂商，产品统一采用塑料PS原料注塑而成，产品的上盖为高透明下盖为黑色，盒子的外表面可根据客户的需求烫金各种图案及文字，盒内可根据客户的要求制作成各种形状的PVC冲片植绒布和吸塑植绒布，广泛用于各种金属礼品和时尚饰品，人造首饰品行业，且用于巧克力食品的包装，我们目前开发了一系列大小规格的塑料盒，该产品透明度高，外观精美，价格合理，交货迅速，品质可靠。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。