查询词典 Western

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

Then the flic gene was cloned into the E.coli expression vector pGEX-KG, which was induced with IPTG and the fusion protein was analyzed by SDS-PAGE and Western-blot.

构建重组表达质粒pGEX-flic，转化大肠杆菌工程菌BL21，经IPTG诱导表达后，通过SDS-PAGE及Western-blo对表达蛋白进行鉴定。

The 528 bp fragment of flic gene was successfully cloned into vector, the pGEX-flic recombinant plasmid was expressed and induced by IPTG and Western-blot method showed good antigenicity of the recombinant protein, and there were flexible regions such as coil region and turn region in flic protein.

获得的目的基因片段长度为528 bp，测序表明其序列与GenBank公布的一致；软件预测结果显示，该蛋白具有多个明显的二级结构成分及跨膜区；SDS-PAGE检测表明，在约50 ku处有一特异性条带；Western-blot检测表明，表达蛋白具有良好的抗原活性。

To avoid some of these complications, neurologistRobert Friedland and colleagues at Case Western Reserve University in Clevelandturned their attention to what people do in their free time.

为了避免这些因素的感染，美国Western Reserve大学的神经学家Robert Friedland和他的同事们并把眼光投向了那些人的业余升后，看看他们的业余时间都在干些什么。

To avoid some of these complications, neurologist Robert Friedland and colleagues at Case Western Reserve University in Cleveland turned their attention to what people do in their free time.

为了避免这些因素的感染，美国Western Reserve大学的神经学家Robert Friedland和他的同事们并把眼光投向了那些人的业余升后，看看他们的业余时间都在干些什么。

Coli mainly in a form of inclusion body. The expressed product contained about 25% of total somatic protein and showed good reactogenicity as proved by Western blot. The IgG level against GAMP induced by GAMP-CRM197 conjugate was significantly higher than those by GAMP and by the mixture of CAMP and rCRM197. Repeated immunization with the conjugate induced immunopotentiation, indicating the effect of CRM197 as a carrier protein.

结果重组CRM197在大肠杆菌中主要以包涵体形式表达，表达量占菌体总蛋白的25%左右；Western blot证明重组rCRM197具有良好的反应原性；各针接种后，结合物诱生的多糖特异性IgG水平均显著高于GAMP组和GAMP+rCRM197混合物组，具有较强的免疫原性；多次接种产生了免疫增强效应，重组CRM197具有载体蛋白的作用。

The expression of TGF-β in glomerulus was examined by immunohistochemistry. Smad2 phosphatase activity was detected by Western blotting.

光镜下观察肾组织的病理改变情况，免疫组化检测肾小球转化生长因子－β1，(TGF-β1)的表达，Western blotting检测Smad 2磷酸化水平。

Methods: The expression of glutamic acid receptor-2 in spinal cord was detected by immunohistochemical method and Western blot analysis.

应用免疫组织化学方法检测GluR-2在脊髓后角的表达，并用图像分析系统和Western印迹法进行定量处理。

Methods: Atrial tissue was obtained from the right appendage during open surgery in 25 patients with RHD. The mRNA of ACE and ERK2 and AT1R was semi-qualified by reverse transcription-ploymerase chain reaction and normalized to the glyceraldehyde 3-phosphate dehydrogenase. Western blotting analysis was employed to examine the expression of ACE and ERK and AT1R. Atrial collagen volume fraction was detected by Masson's stain.

25例风心病二尖瓣狭窄接受外科手术者，手术时取右心耳处心房组织500mg，通过逆转录—聚合酶链反应技术，以GAPDH为内参照测量ACE、AT1R、ERK2的 mRNA变化，用Western Blotting了解ACE、AT1R、ERK在蛋白水平表达的差异，经免疫组织化学了解胶原纤维容积分数改变情况。

The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase in K562 cells was detected by Western blot.

本研究探讨皖南蝮蛇毒蛋白提取物对白血病细胞K562增殖的影响及诱导凋亡的作用，采用MTT法检测不同浓度的蛇毒蛋白提取物对K562细胞生长的影响；应用电子显微镜观察药物作用后K562细胞形态的改变；流式细胞术检测蛋白提取物对K562细胞细胞凋亡作用；Western blot检测细胞增殖酶活性的变化。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。