查询词典 Western

Flow cytometry was used to measure the cells cycle kinetics of the treated cells. Hoechst33258/PI double staining was performed to evaluate the cell morphology. Western blotting was conducted to analyze the expressions of p53, Bcl 2 and Bax. Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells.

以胎盼蓝活细胞拒染法测定药物对细胞生长、增殖的抑制及IC50 ；以流式细胞术观察药物对细胞周期的影响；以荧光双染法（Hoechst33258/PI）观察药物对细胞形态学的影响；以Western blot法观察药物对细胞P53、Bcl 2、Bax蛋白表达的影响。

Methods: Expression levels of MMP10 gene and protein in human spontaneous abortion decidua were determined by RT-PCR, Western blotting and distribution by immunohistochemistry.

利用RT-PCR和Western blotting技术分别检测MMP10基因和蛋白在人正常蜕膜和自然流产蜕膜的表达水平、利用免疫组织化学技术检测MMP10在人正常蜕膜和自然流产蜕膜组织中的分布。

Some of the differential expression proteins were verified by Western blot analysis and immunohistochemical staining, and the results were consistent with 2-DE analysis.

一些差异表达的蛋白质通过Western blot分析和免疫组织化学染色进行了验证，结果和2-DE分析的相一致。

The differential expression level of nm23-H1 in the 2 groups of 5-8F cells were confirmed by Western blotting.

Western 印迹分析证实了nm23-H1在药物处理与未处理5-8F细胞中的差异表达水平。

By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

The results of Western blotting and immunohistochemical method showed that HO-1 protein expression increased in DOX group and DOX -IR group,and was negative in normal group.

Western blotting法和免疫组织化学S-P法检测显示DOX 组和DOX-IR组 HO-1蛋白表达增加，HO-1在正常肝脏内的表达呈阴性。

The expression of GDNF of spinal L4 DRG was determined by Western blot.

采用Western blot法检测脊髓L4背根神经节GDNF的蛋白表达。

Western-blotting confirms, but the shirt-sleeve albumen that dissolve conveys can have good immune reactivity with CCK-8 electropositive serum.

Western-blotting证实，可溶表达的融合蛋白能和CCK-8阳性血清具有良好的免疫反应性。

By action on HNF3β,HNF6 play some role in endoblast differentiation.Reverse transcription PCR can detect HNF6 gene expression in all tissues. Western blot can detect HNF6 protein expression in all tissues.

本研究结合免疫组织化学、逆转录PCR和Western杂交技术，分别在基因和蛋白质水平上初步探索了转录因子FOXF1、Notch2和HNF6在人类肝癌中的表达规律。

Methods The methods of both morphological analysis and cell viabilities were used, and Western blot was also used to analyce the role of antitoxiciy of estrone.

用形态学鉴定和细胞死亡率的测定以及Western blot方法分析雌激素对NMDA诱导神经元凋亡的作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。