查询词典 Western

One such experiment was done by Dr. Roy Baumeister of Case Western Reserve University.

Case Western Reserve大学的Roy Baumeister医生做了一个这样的实验。

The expressed protein was identified by SDS-PAGE and Western blot.

表达的蛋白经SDS-PAGE和Western blot，进行分析。

He proteins of TLR2, 3, 4 and 9 were also comparedby immunofluorescence staining and western blot.

LR2，3，4，9蛋白水平的表达使用荧光免疫组织化学和western blot来检测。

Methods mRNA and protein expressions of TLR4 and TLR9 were detected by RT-PCR and Western blot.

RT-PCR和Western blot法检测TLR4和TLR9在mRNA和蛋白水平的表达情况。

The expressed product was identified by SDS-PAGE and Western blot.

采用SDS-PAGE和Western blot检测目的蛋白的表达。

Result: The fusion protein was identified by SDS-PAGE and Western blot.

结果：表达的融合蛋白经SDS-PAGE和Western blot鉴定无误。

The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明，制得的兔抗PRRSV IgG纯度较高，具有良好的特异性，可用于原位检测病猪肺组织中PRRSV抗原的分布；Envision法的检测结果显示，PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内，其次为肺泡上皮细胞和细支气管黏膜上皮细胞；通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测，均有较高的阳性细胞表达，平均阳性细胞率为46.5%。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Methods Models of severe thoracic trauma were established,alveolar macrophages were obtained by collecting broncho alveolar lavage fluid,and then separated and cultured.TLR4 mRNA and the expression of TLR4 protein were measured by northern and western blot before and 2,4,8,16,24h after trauma.

建立大鼠严重胸部创伤模型，支气管肺泡灌洗分离、培养肺泡巨噬细胞，利用Western、Northern分子生物学技术检测创伤前、创伤后2、4、8、16、24小时肺泡巨噬细胞TLR4的表达水平。

By using the method of Western blot on fractions of nuclear and cytosol of primary cultured mantle cells and immunogold electron microscopy, we found out the completely different localization of these two proteins: CaM was localized both in cytoplasm and nuclear but CaLP was localized only in the cytoplasm.

通过对体外培养的珍珠贝外套膜上皮细胞核质分离后进行的Western blot分析和免疫电镜研究发现，CaM和CaLP的亚细胞定位是截然不同的：CaM分布于外套膜上皮细胞的细胞核与细胞质中，而CaLP却仅分布于外套膜上皮细胞的细胞质中。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。