查询词典 Western

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

Over the past 18 months, Emerson sold several small businesses which included its Western Forge hand tool unit and Buehler materials testing business, and in recent weeks sold its joint venture interest in Industrial Motion Control.

在过去18个月中，艾默生出售了几家小型企业，其中包括其Western Forge手工具业务以及Buehler材料检测业务，并在最近几个星期出售了其在工业运动控制领域的合资公司。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

Western Blot analysis indicated that parvalbumins from four fish (common carp, silver carp, crucian carp, sea bream) can all be detected by the polyclonal antibody specifically.

用制备的多克隆抗体进行Western blot分析，能特异地检测四种鱼（鲤鱼、鲢鱼、鲫鱼、黄鳍鲷）中的小清蛋白。

Results As shown in Western blot analysis, the presence of microsomes did not alter the molecular weight of TM-TNF , but it did result in the cleavage of the IL-2 signal peptide from S-TNFm,suggesting that the leader sequence of TNF might differ from the signal peptide of typical secretory protein in that it seemed not to have undergone cleavage during translation in the rough-faced endoplasmic reticulum.

结果 经Western blot 分析结果表明：微粒体的存在并不改变26×103 TM-TNF的分子量，但微粒体的存在能将S-TNFm上的IL-2信号肽切除，证实TNF引导序列与一般分泌性蛋白的&信号肽&不同，在粗面内质网翻译过程中不被切除。

Methods Mammalian expression vectors containing human MGMT gene were tranferred into PBMC in vitro via liposome. Realtime PCR and Western blot were used to detect the expression of MGMT at mRNA and protein levels.

利用脂质体法将含入MGMT基因的真核表达载体导入体外培养的外周血单个核细胞，实时PCR及Western blot检测目的基因mRNA及蛋白水平的表达。

SLEEPING BAGS: This is one of the items where cost is usually directly proportional to weight and quality. Many manufacturers (such as Western Mountaineering and Feathered Friends to name a few) produce bags which are rated at 20F and are below 2lbs.

睡袋：这项装备的价格通常是重量和质量是成正比的，许多生产厂商(例如： Western Mountaineering 和Feathered Friends)生产的温标为20华氏度的睡袋但重量却低于2磅。

SLEEPING BAGS: This is one of the items where cost is usually directly proportional to weight and quality. Many manufacturers (such as Western Mountaineering and Feathered Friends to name a few) produce bags which are rated at 20F and are below 2lbs.

睡袋：这一项目通常是重量和质量是成正比的，许多生产厂商(例如： Western Mountaineering 和Feathered Friends)生产的睡袋规格为20华氏度且重量低于2磅。

OBJECTIVE: To detect the differential expression of ChM-Ⅰ and CTGF protein in MSCs, hyaline cartilage cells and elastic cartilage cells by Western blot assay.

目的：以Western blot检测软骨调节素Ⅰ和结缔组织生长因子在大鼠骨髓间充质干细胞、透明软骨细胞及弹性软骨细胞中的差异表达。

Methods Physiological and biochemical parameters of the blood were determined to detect liver function. membrane protein and nuclear protein were isolated from the liver tissue of BDL rats. The protein expressions of MRP2 and FXR were determined by Western blot.

用胆总管结扎术方法制备大鼠梗阻性黄疸模型，检测肝功能；提取大鼠肝细胞膜蛋白与核蛋白，测定提取液蛋白浓度；采用Western blot技术检测MRP2和FXR蛋白表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。