查询词典 Western

We employed a cell line of mouse podocytes immortalized with the simian virus 40 large tumor antigen gene, and examined the cell proliferation using colony-forming and MTT assays following treatment with various concentrations of bFGF. BMP-7, alpha-smooth muscle actin e podocytes were detected by western blot. Methyl blue staining was used for the observation of cell morphology.

利用SV40肿瘤抗原转染所获得的永生性小鼠足细胞系培养，以集落形成法和噻唑蓝MTT还原实验测定不同浓度bFGF刺激下的足细胞增殖，采用SDS-聚丙烯酰胺凝胶电泳和Western blotting 检测BMP-7、alpha-平滑肌肌动蛋白、以及波形蛋白的蛋白质水平表达，同时以甲基兰染色观察细胞形态。

Western blot analysis showed that the antibody specifically reacted with the recombinant protein and natural human liver microsomal proteins.

Western blot鉴定表明该抗体可特异性识别重组蛋白以及天然的人肝微粒体蛋白。

Western blot results showed that the antibodies recognized proteins with molecular weight of 63000, 59000 and 53000, respectively, the antibodies could specifically react with synthesized peptides of CYP450 enzymes as well as natural human liver microsomal proteins.

Western blot结果显示，三种抗体均可与其合成肽及天然CYP1A2、CYP3A4及CYP4A11蛋白出现特异性反应，识别的相应抗原的相对分子质量分别为63000、59000及53000。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

Realtime RT-PCR and Western blot were used to test the expression of NSBP1,cyclinB1 and Bcl-2 in the xenografts.

运用实时RT-PCR和Western blot方法检测移植瘤中NSBP1、cyclinB1与Bcl-2的mRNA和蛋白的表达。

Titrations are performed in immuno-fluorescence, ELISA, and western blot so that you may make an educated guess as to the appropriate dilution for your application.

对于免疫荧光，ELISA法和western blot还要进行滴定，让您可以根据您的具体应用进行适当的稀释。

The changes of caspase- 3 protein and the expression of p38MAPK proteins were detected by western blotting at 0,30,60 and 180 min after dexamethasone- induced thymocytes apoptosis.

运用 Western blotting技术检测地塞米松处理的 Balb/c小鼠胸腺细胞 0， 30， 60， 180 min时相点 caspase- 3蛋白与 p38MAPK的表达变化。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

Transforming growth factor-β_1; human retinal pigment epithelium cell ; RT-PCR ; Western-Blot

转化生长因子-β1；人视网膜色素上皮细胞；逆转录多聚酶链反应； Western-Blot印迹法

In this experiment we detected changes of PARP mRNA by means of RT-PCR and PARP protein by means of Western-blot.

在本实验中，采用RT–PCR法检测PARP的mRNA的变化和Western-blot法检测PARP蛋白的变化。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。