查询词典 Western

In this study, the direct interaction between c-Abl and HSP70L1 was demonstrated by co-immunoprecipiation, in vitro binding assay and Far-Western blot.

本研究利用免疫沉淀、免疫印迹、GST-Pull down以及Far Western等方法证明了HSP70L1通过C端的120氨基酸片段与c-Abl在细胞内存在直接的相互作用。

Methods Pull-Down assay in vitro, Far-Western Blot assay and Co-Immunopreci-pitation assay in vivo were used to detect the binding of HBX protien to TF ⅡB.

应用体外蛋白与蛋白结合试验(Pull-Down试验)和Far-Western Blot试验，以及细胞内联合免疫沉淀试验。

The proteins interacting with BMP-2 were screened by AH 109 mating with the bait strain Y 187, and the interaction was confirmed using far-Western blot analysis.

最后用Far-Western blotting法进一步从体外论证BMP-2相互作用蛋白。

After the sequence and open reading frame were confirmed by DNA sequencing, the GADes gene was expressed in E.coli. SDS-PAGE analysis suggested that the bacteria BL21 produce a kind of fusion protein of 92KD and the expressed GADes fusion protein shows expected immunoreactivity by Western Blot.

SDS-PAGE电泳和Western Blot分别鉴定融合蛋白大小和免疫活性后，对表达条件进行优化，对包涵体进行分离、溶解和复性处理，以提高可溶性融合蛋白的产量，使融合蛋白达总蛋白的50％，相当于1.495g/每升培养物。

Methods IUGR model were established by maternal protein-malnutrition. The mRNA levels of TLR4 in hepatics of IUGR rats at 3 weeks of age were analyzed by fluorescent quantization RT–PCR. TLR4 protein levels in hepatics were determined by Western blot. Hepatics TNF-αand IL-1βlevels were measured by ELISA.

采用孕期蛋白质营养不良法建立IUGR大鼠模型，应用荧光定量RT-PCR技术检测3周龄子鼠肝脏中TLR4的mRNA含量变化，Western blot方法检测子鼠肝脏TLR4的蛋白表达，并应用酶联免疫吸附法检测肝组织中肿瘤坏死因子-α和白介素-1β的水平。

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V-Ⅲ by ELISA. Four patients ( 22.2%) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS.

对浙江省1982～1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者，用酶联免疫吸附法检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒抗体，发现4例阳性，并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。

The protein and mRNA expression of PLCγ1 was examined by Western blotting and RT-PCR, and the effect of the lentivirus on cell apoptosis was analyzed by flow cytometry.

应用Western-blot和RT-PCR对细胞中PLCγ1的蛋白和mRNA表达水平进行分析。应用流式细胞仪分析PLCγ1 siRNA对细胞凋亡的影响。

Breast tissues were taken at surgery from patients with breast adenoma, nodule of lobuli glandulae mammariae and breast cancer. Normal samples adjacent to breast cancer were used as control. NIS mRNA and protein were assessed in these samples by reverse transcription polymerase chain reaction , Western blot and immunohistochemistry methods.

1。乳腺组织取自乳腺纤维瘤、乳腺小叶增生以及乳腺癌患者，另取癌旁正常乳腺组织作为对照，分别运用半定量逆转录聚合酶链反应、Western Blot方法和免疫组织化学技术检测NIS的基因和蛋白表达情况。

BTL/Western Electric not only developed and produced magnetrons; they also produced a secession of shipboard fire-control radars in close conjunction with the Naval Research Laboratory.

不单在磁控管的研究与制造方面，BTL/Western Electric与NRL在舰载火控雷达上有非常紧密的合作。

A recent study conducted by the researchers at the Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Ohio, it is found that prolonged mobile phone use may damage sperm cells in male users.

最近的一项研究进行的研究人员在克利夫兰诊所勒纳医学院， Case Western Reserve大学，俄亥俄州，它是发现，长期使用手机可能会损害男性精子细胞的用户。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。