查询词典 Western

It was found that the fusion protein has good antigenicity by Western-blot.

Western印迹分析结果说明该基因在大肠杆菌中的融合表达产物rSjGcp具有良好的抗原性。

Western blotting showed that the recombinant protein had good antigenicity.

Western印迹分析结果说明该基因在大肠杆菌中的融合表达产物rSj423具有良好的抗原性。

Western blot was used to identify the antigenicity and immunoreactivity of rFimA.

采用Western blot鉴定rFimA抗原性和免疫反应性。

Its antigenicity was identified by Western blot.

Western blot鉴定重组HpaA蛋白的抗原性。

The titer and specificity of murine antiserum was evaluated by ELISA, Western blot and immunofluorescent staining.

用ELISA、Western blot和免疫荧光分别检测抗血清的效价和特异性。

SDSPAGE and Western blot analysis showed that HSA/M2e was successfully expressed in the culture medium with an apparent molecular weight of 68 kD.

SDSPAGE和Western印迹分析证实了甲醇诱导的培养基上清中含有HSA/M2e，分子量约为68 kD。

The results are as follows: 37℃ is the most favorable temperature for induction expression of SUMO-hEGF in BL21, 5.0g/L the most best concentration of arabinose, and 4h the best time for induction expression. Meanwhile, the quantity of expression is around 20.2%. The conclusion that hEGF is the purified protein is verified by Western blotting. It is a good foundation for the development of hEGF genetic medicine.

结果表明： SUMO-hEGF在BL21中的最佳诱导表达温度为37℃、诱导剂阿拉伯糖的最佳浓度为5.0g/L、最佳诱导表达时间为4h，表达量约为20.2%；Western blot 分析证实，纯化后的蛋白是hEGF，为进一步开发hEGF基因工程药物奠定了基础。

Methods The expression of COX-2 in 10 specimens of normal oral mucosa, 30 specimens of OSF and 15 specimens of oral squamous cell carcinoma by SP immunohistochemistry. The COX-2 protein induction by arecoline in different concentration (30, 60, 90 uglmL), was analyzed by Western blotting in normal human oral keratinocyte.

应用免疫组化SP法检测15例OSF癌变组织，30例OSF组织，10例正常口腔黏膜中COX-2蛋白表达，western-blot法检测不同浓度槟榔碱(30、60、90ug/mL)对KC COX-2蛋白表达的影响。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Western blot analysis shows that the protein could be identified only by the antiserum to M. bovis but not the sera against.M, paratuberculosis, brucellosis, babesia and infectious bovine rhinotracheitis virus.

Western blot分析显示：该融合蛋白能与抗牛分枝杆菌阳性血清发生特异性反应，而与牛副结核病、牛布氏杆菌病、牛巴贝斯病和牛传染性鼻气管炎病阳性血清不反应。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。