查询词典 Western

You can buy from this website by using a Credit Card, wire funds to our Bank Account, send money via Western Union or Email funds from any Canadian Bank account.

你可以买到从本网站的使用信用卡，电汇资金，我们的银行帐户，汇款通过Western Union或电邮资金来自任何加拿大的银行帐户。

The expressions of HIF-1αprotein were detected by Western blot, and we selected a properly cobalt chloride density and culture time as the most suitable culture condition.

1、用不同缺氧时间(0、12、24、48、72h)、不同浓度氯化钴(0、200、400、600、800μM)模拟缺氧培养食管鳞癌细胞Eca-109，以Western blot法检测细胞中HIF-1α蛋白的表达变化，选择可以引起HIF-1α蛋白表达增加的适当的氯化钴浓度和缺氧培养时间。

The protein expression level of Syk was lower than the control by Western Blot assay,but that of IFN-γwas higher.

Western Blot提示：试验组SYK蛋白表达降低，而IFN-γ蛋白表达增高。

In an interview before the second case was found, Dr. Linda A. Detwiler, who retired in 2002 asthe chief of the mad cow testing program and now teaches veterinary medicine at the University of Maryland, said the department should be using the Western blot test it was resisting.

在第二个案例被发现前，於2002年退休、目前任教於马里兰大学教授兽医药学、当年是狂牛症检疫计画主任的林达博士，说农业部应该使用该部一直抗拒使二的&Western bolt&检验法来做检验。

After the stimulation of LPS, the expression of CXCR4 and the concentrations of TNF-α and IL-6 in culture fluid were detected through the methods of Western Blot, immunohistochemistry and RT-PCR respectively.

在LPS刺激肺泡巨噬细胞后，分别应用ELISA、Western Blot及RT-PCR法检测肺泡巨噬细胞培养液中TNF-a和IL-6浓度以及肺泡巨噬细胞CXCR4蛋白和mRNA表达的变化。

The antisera were characterized by Western blot using MGr1 antibody as positive control.

6以MGr1抗体为对照，用Western blot鉴定制备的抗血清。

The immunoreaetivities of the prepared antisera with recombinant and native PrP proteins were evaluated by ELISA and Western Blot assays.

ELISA和Western Blot检测所制备抗体与重组和天然的PrP蛋白的免疫反应性。

To evaluate the specificities of the resultant antisera, bacterial lysates of seven NM strains were subjected to Western blotting.

为了评估得到的抗血清的特异性，以各抗血清为探针对七株不同的NM菌进行Western blotting。

To answer these questions, in the present study, an attempt was made to examine:(1) the GABA responses in mechanically dissociated Vme neurons with attached synaptic boutons by using the whole cell patch clamp technique;(2) the change of expression of KCC2 and NKCC1 during the postnatal stage in the Vme by using the Western blotting technique;(3) the percentage of colocalization of PV with KCC2 or NKCC1 during the postnatal stage in the Vme by using the double immunofluorescence histochemical techniques.

基于此，本论文进行了如下研究：(1)运用机械分离带终扣的单细胞膜片钳技术对大鼠Vme神经元对GABA的反应进行观察；(2)运用Western blotting技术结合图像分析方法观察生后发育过程中Vme神经元内NKCC1和KCC2的表达变化；(3)运用免疫荧光组织化学双重标记技术对生后发育过程中Vme神经元内NKCC1和KCC2与PV之间的共存关系进行了系统观察。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。