查询词典 Western

The expression levels of Bcl-2 protein were assayed by Western-blot and cell proliferation was measured by MTT method.

通过倒置荧光显微镜和流式细胞仪观察细胞的转染效率，Western印迹法检测Bcl-2蛋白表达水平，MTT法测定细胞增殖情况。

Western blot assays were used to detect the expression of βNGF.

Western Blot检测蛋白的表达情况。

Furthermore, the expression of bFGF and angiogenin was examined by RT-PCR and Western blot assays.

接着用RT-PCR和Western blot的方法检测了bFGF和血管生成素的表达情况。

The gene expression of orexin in rat hypothalamus tissue was examined by real-time PCR and Western blooding analysis.

应用实时定量PCR和Western 印迹杂交分析检测下丘脑组织中orexin的mRNA和蛋白质在急性低血糖应激状态下的表达改变。

The effects of different renaturation agents, blockings and labelled conjugates were studied to improve the technique of non-radioactive Western Blot.

应用不同蛋白质复性剂、封闭剂和标记物的对比方法以探讨Western印迹技术的改进。

Coli BL21 (DE3) plysS for expression under induction of IPTG. The expressed product in a form of inclusion body was denaturalized with urea, purified by nickel ion chelate affinity chromatography, renaturalized by gradient dialysis and identified by Western blot.

coli BL21(DE3) plysS进行诱导表达，采用Ni(上标 2+)螯合亲和层析法纯化经尿素变性的包涵体，梯度透析复性纯化的目的蛋白，并进行Western blot鉴定。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

Sheep were immunized with DNA vaccine and antibody production was analyzed by western blot and ELISA.

将纯化的DNA疫苗免疫绵羊后，用western blot和ELISA方法检测疫苗在绵羊体内的翻译和诱导IgG的产生。

Based on establishment of the human macrophage-derived foam cell model, the expression of Kv1.3 channel was investigated by immunocytochemical staining, reverse transcription-polymerase chain raction and Western blot. Furthermore, the effects of rMargatoxin, a Kv 1.3 channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein were studied.

在建立人巨噬细胞源性泡沫细胞模型的基础上，采用免疫细胞化学染色法、逆转录聚合酶链反应(transcription-polymerase chain raction，RT-PCR)及Western blot，研究Kv1.3通道的表达，并观察其特异性阻断剂--rMargatoxin对摄取氧化修饰低密度脂蛋白的巨噬细胞胆固醇代谢的影响。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。