查询词典 Western

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Akt,AMPK and ERK proteins were detected by western blot analysis,and the distribution of GLUT4 in these skeletal muscles was observed by immunofluorescence. Results The expression of GLUT4 on the cell membrane was significantly higher in the electrically stimulated groups than in the unstimulated groups.

用Western Blot法测定骨骼肌中蛋白激酶B(protein kinase B，PKB/Akt)、腺苷酸激活的蛋白激酶、细胞外信号调节激酶的表达和活性变化，用免疫荧光方法观察GLUT4在细胞膜及细胞内膜的分布。

In Western blotting analysis, P6 was specifically detected in the RBSDV-infected maize leaves and the viruliferous planthoppers, while anti-P6 antisera did not react with any protein from purified RBSDV particles.

Western blotting分析显示，P6抗血清不与纯化的RBSDV粒子蛋白发生反应，而在被RBSDV感染的玉米叶片和带毒的传播介体－灰飞虱中可检测到P6。

Program last year, Case Western Reserve University's Weatherhead School of Management in Cleveland restructured classes by themes -- the language of business, stakeholder management, global business, processes and systems, and execution.

为了使自己的管理人士MBA课程恢复活力，凯斯西储大学(Case Western Reserve University)Weatherhead管理学院按教学内容分类对课程进行了重组，重组后的课程包括商务语言、利益相关者管理、全球商务、过程与系统以及执行。

For GM to play that role, however, it will be important for the company to be careful which workers it cuts while it's in bankruptcy, said Susan Helper, an economist at Case Western Reserve University's Weatherhead School of Management.

不过，凯斯西储大学(Case Western Reserve University)维德罕管理学院经济学家赫尔珀说，通用汽车要想发挥这一作用，重要的是在破产中认真考虑要裁减的人员。

Then nuclear transcription factor expression level of chop in each group was detected with western blot.

以western blot法分析各组心肌中核转录因子chop的表达水平。

IHC, western-blot and immunofluorescence staning revealed that the levels of ILK and ICAM-1 up-regulated 6 h after RPE cells cultured with high glucose (30 mmol/L). Real time RT-PCR analysis showed that the levels of ILK mRNA increased at 2 h, and then decreased at 6 h.

免疫组化、western-blot和免疫荧光染色都显示RPE细胞在高糖培养6 h高表达ILK、ICAM-1蛋白，而实时荧光PCR检测显示在2 h其mRNA表达量开始上调，在6 h下降。

Western Blot justified that the low expression of sirtl gene after γ radiation plays a role in the high acetylated

Western Blot实验证明，sirtl基因在γ辐射后表达下调可能与p53的乙酰化水平升

For further analysis, express of Bcl-2 were assayed by Western blot.

以Western blot印迹法检测Bcl-2蛋白的表达。

EMMPRIN mRNA and protein expression were assayed by RT-PCR and Western blot.

应用RT-PCR和Western blot测定巨噬细胞、泡沫细胞中EMMPRIN基因和蛋白的表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。