查询词典 Western

Methods The expression of Rhbg in cortex, outer medullar and inner medulla of the kidnty was detected by Western blotting.

利用Western印迹分别检测Rhbg在低蛋白饮食组和正常对照组大鼠肾脏皮质、内髓、外髓的表达。

Results: The expression of HO-1 mRNA in macrophage cell was significantly enhanced by the melilotus suaveolens extract. The result of Western blot showed that the expression of HO-1 protein was significantly increased after the intervention of melilotus suaveolens extract.

结果：臭苜蓿根提取物明显升高巨噬细胞HO-1的mRNA表达水平，Western blot结果显示，臭苜蓿根提取物干预后HO-1的蛋白表达水平也明显升高。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

To study the role of FoxO1 on myoblast proliferation and differentiation and the transformation of muscle fiber type in porcine,here we use yorkshire as experimental materials and detect the expression of genes related myogenesis and muscle fiber type in pporcine myoblast that was knockout or activated FoxO1,adopting the technology of RNAi,real time-PCR and Western blotting et al..

为探讨FoxO1对猪成肌细胞增殖分化及肌纤维类型转化的作用及机理，本试验以大白猪为研究材料，利用RNAi、real-time PCR、免疫细胞化学和Western blotting等技术，通过在原代培养的猪成肌细胞中敲除和激活内源活性FoxO1，检测肌肉形成与肌纤维类型相关基因的表达。

Western blot proved good specificity of expressed Nef protein.

Western blot检测显示了良好的特异性。

Recombinatino polypeptide or chemically synthesized polypeptide antigen is used to immunize the mammal to obtain polyclonal antibody and the nonconservative amion acid sequence of the antiboby to N-terminal with recognition site qBrn-2 of development regulatory protein qBrn-2. The antibody does not recognize other POU structure domain protein, and recognizes specifically qunique protein in Western imprinting experiment. The qBrn-2 expression mode obtained immunohistochemistry process is identical with the resulted height of in-situ molecular hydridization.

以重组多肽或化学合成多肽为抗原免疫哺乳动物获得的多克隆抗体，这一抗体对发育调控蛋白qBrn－2的识别位点为qBrn－2的N－末端的非保守性的氨基酸序列，该抗体不识别其它POU结构域蛋白；在Western印记实验中，该抗体特异地识别单一的蛋白；利用免疫组织化学接示的qBrn－2表达模式与分子原位杂交的结果高度一致。

After 200 days clinical observation, brain, cerebellum and obex were examined by Western Blot to find the enzyme-persistent prion protein and by real-time PCR to detect the prion gene expression.

临床观察200天后，对大脑、小脑和脑干，进行蛋白酶抗性朊蛋白的Western Blot检测和朊蛋白基因表达绝对定量。

In this experiment, the object was ovaries from adult mouse, we use western blot technique and immuno-histochemistry method to study and research expression and distribution changes of ERβand p-ERβduring oestrous cycle and early gestational period (1~11d).

本实验以成年雌鼠卵巢为实验材料，分别采用western blot技术和免疫组织化学方法，研究ERβ和p-ERβ在小鼠卵巢各发情周期和妊娠早期(1~11d)表达量与定位分布的变化规律。

The results during oestrous cycle of western blot showed that in mouse ovary ERβand p-ERβwere highly expressed at oestrus, but there was no significant deviation at other periods.

发情周期的western blot实验结果表明：ERβ和p-ERβ在小鼠发情期卵巢中均呈现高水平表达，而在其它时期两者的表达量均较低，且各自相互间差异不显著。2。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。