查询词典 Western

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

Using immunohistochemical technique and image analysis system, Western blot, we observed that the level of HBcAg expressed in endocellular went down.

ELISA方法检测发现核酶抑制HBeAg表达48.6%，用免疫荧光、免疫组化、图象分析法、Western blot分析证实核酶可抑制HBV细胞内表达。

The purified PAT was identified by Western blot and enzymic activity analysis.

首先通过Western杂交和酶活性分析鉴定了PAT蛋白的纯度和活性。

The expression level and enzymic activity of transgenic rice were evaluated by Northern blot, Western blot and the enzyme reaction.

用Northern blot ，Western blot和酶活测定的方法检测了转基因水稻中几丁质酶和葡聚糖酶的表达和活性。

These proteins were cloned and expressed fragmentally. The positive band in the Western blot showed that the second section of the integral membrane protein (IMP2) was the target protein.

并进行克隆表达及分段表达，并在此后的Western blot鉴定中呈强阳性条带显示靶蛋白是整合膜蛋白的第2段。

MAIN OUTCOME MEASURES: The expression of VEGF in rats' gastrocnemius muscles was detected by using western-blot.

主要观察指标：用Western-blot法测定大鼠腓肠肌血管内皮生长因子表达。

Professor David Candy, a consultant paediatric gastroenterologist at Western Sussex Hospitals, said his team had tried hypnosis in a small group of children with severe abdominal pain problems and had 100% success rate.

Western Sussex 医院的儿科、消化科顾问 David Candy 教授说，他的团队尝试对一小组有剧烈腹痛的孩子们使用催眠疗法，取得了100%的成功率。

The expressions of t bet and gata 3 mrna and protein in the lungs were detected by reverse transcriptase polymerase chain reaction and western blot respectively.

用逆转录聚合酶链反应测定法和western blot法分别检测t bet， gata 3 mrna和蛋白在肺组织的表达。

Western blot and enzymatic assay indicated that the HL gene and germin gene was expressed in tobacco cell respectively.

Western杂交和草酸氧化酶活性检测分别证明了双价基因中的HL和germin在烟草中的表达。

Methods MTT assay for detection of single use of Adriamycin and combination of Adriamycin with gleditsia saponin on the Role of the SMMC-7721 cell survival rate; detection of mRNA expression of bcl-2 by PCR method; detection of protein expression of bcl-2 by Western blotting method.

MTT法检测单独应用阿霉素或阿霉素与皂角皂苷联合作用的SMMC-7721细胞的生存率；RT-PCR检测bcl-2 mRNA表达；Western blotting检测bcl-2蛋白的表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。