查询词典 Western

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

Equal amount of absolute alcohol was given to the control group. The expressions of vitamin D receptor in HL-60 cells of each group were identified by Western blot analysis, and cellular morphological changes were observed after being stained by Wright-Giemsa.

Western印迹技术鉴定维生素D受体在各组HL-60细胞的表达；Wright-Giemsa染色观察细胞形态学变化；流式细胞术检测细胞表面标志物CDl4抗原的表达；反转录-聚合酶链反应法分析c-myc基因的表达。

Methods Serum IGFBP-3 levels in 20 normal adults, 33 active acromegaly and 34 patients with idiopathic growth hormone deficiency were measured by Western blot.

本文采用Western印迹方法测定20例正常人，33例活动性肢端肥大症病人和34例特发性生长激素缺乏症病人血清IGFBP-3水平。

Western blot and double staining analyses show NYD-SP27 becomes detached from sperm, as they undergo capacitation and acrosome reaction.

Western blot和双染的研究表明在精子发生获能和顶体反应的过程中NYD-SP27逐渐从精子上丢失。

METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to create diabetes. At 2 week after the models were created, BDNF protein was administered into the vitreous cavities of rats. At 4 week after the models were created, the rats were killed and the retina was removed for Western blotting and Whole-mount immunohistochemistry for TH to observe the changes of TH and dopaminergic amacrine cells in retina.

9周龄Wistar大鼠，链脲佐菌素（streptozotocin，STZ）腹腔注射，制成糖尿病模型，于模型建立2wk后，大鼠眼玻璃体内注射BDNF溶液，于模型建立4wk后，处死大鼠，取出眼球，取视网膜组织进行Western blotting及视网膜铺片免疫组织化学染色检测，观察视网膜中TH水平的变化，从而反映糖尿病大鼠视网膜中多巴胺能无长突细胞水平的变化。

Using immunohistochemistry and western blotting, to detect the expression of glucocorticoid receptor in the central amygdaloid nucleus of respective rat groups, and image analysis and statistical analysis were performed.

应用免疫组化、Western Blotting定位定量检测各组CeA的GR表达，进行图像分析和统计学处理。

ELISA and Western blotting showed that the recombinant protein has good antigenic.

ELISA和Western blotting实验证实，重组蛋白具有良好的抗原性。

In vivo, CXCR4-A549 and A549 cells were injected subcutaneously into nude mice(each group in 6).Four weeks later the mice were scarified and the tumors in situ and the lungs were taken out to be examined histologically.(3) Th cell cycle distribution was measured by flow cytometry to analyse the effect of SDF-1/CXCR4 in the cell proliferation and atoposis. The changes of the intracellular calcium concentration after SDF-1 activation were aslo measured by flow cytometry. Western blot was used to anlayse the phosphorylation of AKT and ERK, and the expression of MMP-2 and VEGF-C.

3为探讨SDF-1/CXCR4在肺癌侵袭和转移中的作用机制：①利用流式细胞仪检测CXCR4-A549与A549两组细胞的增殖和凋亡，分析上调CXCR4的表达后，对细胞生长周期的影响；②利用流式细胞仪检测两组细胞钙离子内流强度，分析SDF-1/CXCR4对钙离子通道活性的调控；③采用Western blot实验检测两组细胞PI3K/AKT、MAPK/ERK相关信号通路的活性，分析SDF-1/CXCR4可能激活的下游信号分子；④采用Western blot和RT-PCR检测两组细胞肿瘤转移相关基因VEGF-C、MMP-2的表达，探讨SDF-1/CXCR4与VEGF-C、MMP-2的相互作用关系。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。