查询词典 Western

Methods RT-PCR, Western blot were used to detect the expression of bax in human gastric cancer cells line SGC-7901, and observe the effect of rhIL-24 on chorioallantoic membrane.

采用RT-PCR法和Western blot法检测rhIL-24对胃癌细胞株SGC-7901中促凋亡因子bax表达的影响，鸡胚绒毛尿囊膜技术观察其对血管形成的影响。

Select chorioallantoic membrane vascular extract protein of different time points , Detect FGF2 and TGF-β2 protein in chorioallantoic membrane of this three-stage by Western blotting, judge whether to establish a model of dynamic vascular development successful.

选取不同时间点的尿囊膜血管，提取蛋白，用Western blotting技术检测FGF2和TGF-β2蛋白在血管发育的这三个阶段的表达量的变化，判断动态血管发育模型是否建立成功。

The immunological competence of the expressed protein was tested by means of Western blotting and enzyme-linked immunosorbent assay, and its biological activity was assayed by chicken chorioallantoic membrane and Matrigel angiogenesis assay.

酶联免疫吸附和Western blot技术检测纯化的VEGF165蛋白免疫学活性，鸡胚尿囊绒毛膜实验和matrigel血管形成实验检测其生物学活性。

Methods A plasma clot clonal assay, immunocytochemical staining and Western blot were used in this study.

用血浆凝块巨核细胞集落培养，观察对生长的影响，并进一步使用免疫组化和Western blot证实受体的存在和对c-fos表达的影响。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

PCR technique was used to identify the genotype of the model mice and expression of βB2 crystallin protein was detected by Western blot.

采用PCR对小鼠基因型进行鉴定，Western印迹法对晶体蛋白的表达进行鉴定。

In TUNEL assay it can be observed that cystamine can reduce myocardial cell apoptosis . At the same time Western Blot analysis to prove cystamine can reduce cardiac cell apoptosis and Deth receptor and Mitochondria through theses two pathsway, NF-κB protein expression significantly increased furthermore, it promots cell survival, which can reduce cardiac hypertrophy,apoptosis.

在TUNEL assay中可以观察到胱胺可以减少心肌细胞的凋亡，同时我们也利用Western Blot证明胱胺可以减少心肌细胞的凋亡是透过Deth receptor和Mitochondria这两条路径，而这样子降低凋亡的现象很有可能与NF-kB这讯息传递的分子上升有关，因此胱胺确实具有保护心肌细胞的作用，可改善心肌细胞肥大、凋亡的情形。

The mice were killed 3, 7 and 14 days after operation (8 mice per group at a time), the tissue of cystiform was harvested to receive gross, histology and immunohistochemistry observation, as well as RT-PCR and Western blot detection.

术后观察动物一般情况，于给药后3、7 及14 d，每组取8 只小鼠背部囊壁组织行大体、组织学观察及免疫组织化学、RT-PCR、Western blot 检测。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

Methods The ROS production in the cells was detected by using 2,7-dichlorofluorescin diacetate as probe and the protein expression of the AP-1 and ERK2 determined by Western blot.

用分子探针2，7-DCF测定细胞内ROS的产生量以及用Western blot测定ERK2激酶和转录因子AP-1的蛋白表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。