查询词典 Western

Three different antiserum, rabbitantiserum against formalin-killed-cells of V. parahaemolyticus zj2003 (apathogenic strain isolated from diseased large yellow croaker, from Xiangshan bay,Zhejiang province), rabbit antiserum against OMP of the bacteria and large yellowcroaker convalescent antiserum were prepared for western blotting of the OMPpreparation.

以饱和硫酸铵梯度盐析法结合琼脂糖柱sephadex G200凝胶过滤法初步纯化了大黄鱼血清免疫球蛋白，再经割胶回收法得到进一步纯化的重链片段，分子量约为75kDa，免疫新西兰兔后获得兔抗大黄鱼免疫球蛋白血清，为ELISA和Western blotting检测技术奠定了基础。

Methods 0, 2.5, 5, 10, 15 and 20 μmol/L arsenious acid-treated human hepatocellular carcinoma HepG2 cells were cultured under hypoxia for 8 hours. The cell proliferation and cell viability was assayed by WST-8 and the trypan blue dye exclusion method. The expressions of HIF-1α in human hepatocellular carcinoma HepG2 cells were checked by RT-PCR and Western Blot.

以0、0.25、0.5、1、2和4μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧环境中培养8h；1μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧培养0~10h，WST-8法和台盼蓝染色法分析细胞增殖和活力，采用RT-PCR和Western Blot方法检测肝癌细胞中HIF-1α的表达。

RT-PCR and Western blotting results showed that the transgene was expressed in the liver of the transgenic mice.

RT-PCR和Western blotting结果显示，外源基因在转基因小鼠的肝组织成功表达。

The expression of p27kip1 protein was detected by Western blotting.

Western印迹检测p27kip1蛋白的表达。

Protein and RNA expression were analyzed by Western blotting and RT-PCR, respectively.

蛋白质和RNA表达分别用Western blotting和逆转录PCR技术进行分析。

The expression of PPARγ mRNA and protein were measured by RT-PCR and Western blotting separately.

用RT-PCR方法及Western blotting方法分别检测PPARγmRNA和蛋白的表达。

Western Blotting analysis of antibody specification was successful.

Western Blotting检测抗体的特异性较好。

The levels of PAI-1 protein and mRNA were analyzed with Western blotting analysis and RT-PCR respectively.

采用MTT和流式细胞仪检测细胞增殖，Western blotting和RT-PCR法检测PAI-1的表达水平。

The protein and mRNA expressions of Bcl-2 and Bax were detected by Western blotting and RT-PCR, respectively.

Western blotting和RT-PCR检测各组细胞不同时点Bcl-2和Bax蛋白、mRNA的表达。

Moreover, the protein expressions of all kinds of integrin subunits were also detected with Western blotting.

同时用Western blotting检测各种整合素亚单位的蛋白表达变化。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。