查询词典 Western

To isolate mononuclear cells from foetus umbilic cord blood, After MNCs were incubated in the presence of APS group (APS 200 mg·L-1) and control group for 24 h,the cells were stimulated with Epo（5 IU/mL） for 0,2,5,30 min; STAT5 was measured by ICC and laser confocal scanning microscope, JAK2、STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL in each group at different groups.

分离纯化胎儿脐带血单个核细胞，在常规体外培养体系加入APS（200 mg·L-1）作用细胞24 h，促红细胞生成素分别刺激0，2，5，30 min，免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达；Western-blotting-增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

Western Blot analysis also demonstrated that level of ANXA1 in the villus was up-regulated.

Western Blot分析ANXA1蛋白质的表达，结果与RT-PCR一致。

The coordinators of the network will be Professor Li Shi and Professor John Whalley (University of Western Ontario, Canada), and there will be an organizing committee with 7 Chinese representatives and 3 international representatives.

研究网络的协调人为李实教授与 John Whalley 教授(加拿大西安大略大学 University of Western Ontario， Canada)，并由7名中方代表和3名国际代表组成组委会。

Results of Western-blot reveal: along with the increase concentration of taxol, the expressions of Fas、FasL proteins increased gradually, while the expressions of Caspase-8、Caspase-3 zymogen proteins decreased gradually.

Western-blot结果显示：随着紫杉醇浓度的增加，Fas、FasL蛋白表达量逐渐增加，Caspase-8、Caspase-3酶原蛋白表达量逐渐减少。

Methods Using Western ligand blot technique, the authors measured plasma levels of IGFBP-1-4 in uremic acidotic rats and uremic but without acidotic rats, in the non-uremic acidotic and pair-fed rats and also delermined liver mRNA in UA and UB rats.

采用Western ligand blot等方法，对随意进食组、尿毒症酸中毒组及其对照组尿毒症未合并酸中毒组、非尿毒症酸中毒组及其对照组无酸中毒组的大白鼠进行了血清IGFBP-1-4的测定，对UA、UB组肝脏IGFBPs mRNA进行了测定。

And then the expressing level of AcrAB was detected by Western blotting. The results indicated that the level of resistance to fluoroquinolones of isolates appeared closely correlated with substitution in GyrA and ParC, but expressing level of AcrAB had not significant changes.

序列分析及AcrA的Western blotting检测结果表明，临床分离的鸡大肠杆菌对喹诺酮类药物的耐药水平与GyrA和ParC的突变密切相关，而AcrAB外输泵的表达水平无显著变化。

The result indicated: antibacterial concentration of inner thallus increased progressively with time prolongation when added CCCP with antibacteria, proved that multi-resistance of E.coli in livestock and avian is mediated really by active efflux system; The multi-resistant level have positive correlation with AcrAB transcription and expression level; Quantitive RT-PCR,Western-blotting and fluorescence quantitive PCR can use to detect AcrAB.these results provide theory foundation to detect and study multi-resistance of E.coli in veterinary clinic and have a important scientific significance to develop neotype antibacterials.

结果表明，多重耐药株在有CCCP 存在时，菌体内抗菌药物的浓度随时间的延长而递增，证明畜禽大肠杆菌多重耐药性确实由主动外排系统介导；其多重耐药水平与AcrAB的转录、表达水平呈现正相关；定量RT-PCR、Western-blotting、荧光定量PCR可用于AcrAB水平的检测。这些结果对检测临床大肠杆菌的多重耐药水平、对研究兽医临床大肠杆菌多重耐药机制提供理论依据，对开发新型抗菌药物有重要的科学意义。

Western blot assay indicated that the crude extract reacted with serum obtained from sixteen Amaranthus spinosus pollen-allergic subjects and contained four allergen bands in total, and that the 78 kD and 40 kD bands were the major allergenic components.

Western blotting结果表明，16份刺苋花粉过敏患者的血清全部呈阳性反应，浸出液中共有4条致敏条带，其中主要致敏蛋白的分子量是78 kD和40 kD。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。