查询词典 Western

Methods The mRNA and protein expression levels of NCAM were detected by reversed transcriptive polymerase chain reaction and Western blot after the rat hippocampal neurons were incubated with 20, 40 and 80μg/ml sodium fluoride for 24 hours in vitro.

原代培养海马细胞暴露于20、40、80μg/ml氟化钠24 h后，用RT-PCR和Western blot方法分别检测细胞内NCAM mRNA和NCAM各蛋白亚型的表达。

Methods: hypoxia model of detrusor smooth muscle cell in vitro was established by sodium hyposulfite which consumed the oxygen of the medium. the cx43 and cx43 mrna were detected by western blot and rt_pcr. the level of malondialdehyde was detected.

体外培养膀胱逼尿肌细胞，用连二亚硫酸钠清除培养基内的氧，建立缺氧模型，通过rt_pcr、western blot检测正常对照组、缺氧组及vite预处理组的逼尿肌cx43表达情况，并检测丙二醛质量浓度。

AIM: To observe the effect of solanine on the contents of caspase-3 and Bcl-2 in HepG2, and to explicate the mechanism by which solanine induces the apoptosis of tumor cells. METHODS: Laser confocal scanning microscopy and Western blot were used to measure the contents of caspase-3 and Bcl-2, and LCSM was used to determine their locations in the cell. RESULT: Solanine markedly increased the content of caspase-3 while decreasing that of Bcl-2 in HepG2, both in a dose-dependent way.

目的：观察龙葵碱对HepG2细胞内caspase-3及Bcl-2蛋白含量的影响，阐明龙葵碱诱导肿瘤细胞凋亡的作用机制方法：采用激光共聚焦扫描显微术和Western blot法检测caspase-3和Bcl-2蛋白含量，并对二者在细胞内的位置进行定位结果：龙葵碱能够显著升高跳西2细胞内caspase-3蛋白含量，降低Bcl-2的含量，并且均具有剂量依赖性。

Although you might imagine that type of music he tried, although you can imagine all types of movies he has done music, but his most famous "spaghetti Western" style is for the director Sergio Leone film background music and fame.

虽然你可能想像到的音乐类型他都尝试过，虽然你所能想象到的所有类型的电影他都做过配乐，但他最著名的"spaghetti Western"风格却是为导演Sergio Leone制作的电影背景音乐而成名的。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

At passage 3, cells were induced by 3 mmol/L sodium butyrate and 0.1% dimethyl sulfoxide for 5 days. Cells were harvested for Western blotting assay.

细胞培养到第3代时，分别用3 mmol/L丁酸钠盐与0.1% DMSO进行诱导，5 d后收集细胞进行Western blotting实验。

Chimeric proteins were expressed in COS-7 and CHO cell culture supernatant and confirmed by ELISA and Western blot.

ELISA 和Western blot确定在COS-7和CHO细胞中表达了CD40-Ig融合蛋白。

On Day 5, the spinal cords were removed and prepared for Western blot analysis of glutamate dehydrogenase and glutamate synthetase.

第五天，取髓作谷氨酸脱氢酶和合成酶的 Western 印记分析。

A non-back-ground reaction Western blot procedure for using polyclonal antibodies to study the proteins of citrus tristeza virusCTV was developed.

本文利用多克隆抗体发展了一种无背景的Western blot技术并用于研究柑桔速衰病毒CTV的蛋白。

Methods SP-A and SP-D were determined in the pleural effusions from 36 patients with lung adenocarcinoma and 28 with tubercular pleurisy by western blotting.

方法收集36例原发性肺腺癌性和28例结核性胸腔积液患者的胸水，采用 western blotting检测胸水中肺表面活性物质相关蛋白-A、D。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。