查询词典 Western

Methods: The experimental g roup consisted of 24 patients with PE and 24 cases of normal placentae women respectively. Group of 24 patients with PE consisted of 12 mild cases and 12 severe cases. RT-PCR and Western Blotting were used to test the difference content of HLA-G between two groups.

采用半定量逆转录-聚合酶链技术及Western印迹技术分别检测了24例子痫前期患者(实验组，轻度子痫前期12例，重度子痫前期12例)，24例年龄相仿的正常晚期妊娠者胎盘组织中HLA-G的表达差异。

Nuclear Run-on Assay was used to address the contribution of transcriptioninitiation to the regulation of five histones by YSV. Actinomycin D inhibitor studies were used to detect the mRNA half-life of five histones. SLBP weremeasured by Western blot assay to identify regulation of YSV on Pre-mRNAsplicing.

应用Nuclear Run-on Assay观察YSV对人肝癌BEL-7402细胞的五种组蛋白转录效率的影响；应用放线菌素D转录抑制法观察YSV对组蛋白mRNA半衰期的影响；应用Western blot检测SLBP的表达水平观察YSV对组蛋白mRNA加工的影响。

Levels of Giα protein in sarcolemma were measured by Western blot analysis,and adenyl cyclase activities were determined by radioimmunoassay.

采用Western 杂交法和放射免疫法分别测定大鼠心肌Giα及腺苷酸环化酶的活性。

SDS-PAGE and Western blot of culture supernatant showed that hsIL-1RⅠ was secretively expressed. Recptor-ligand binding assays showed that the recombinant hsIL-1RⅠ has receptorligand binding activity.

发酵液经SDS-PAGE和Western blot证实分泌表达了sIL-1RI，经蛋白印迹受体配基结合反应证实分泌表达的sIL-1RI具有配基受体结合的生物学活性。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

Methods: The traditional method of chronic serum sickness was modified by adding followings: one kidney removed, intraperitoneal inject LPS etc., and the effect of Shenhua Tablet on 24h proteinuria, blood biochemical indices, kidney function, pathology and immunofluorescence changes was observed compared with Fosinopril, and and the effect of Shenhua Tablet on the ED-1 positive cell number in the glomerulus, protein and mRNA expression and activation of Stat3 and protein and mRNA expression of TIMP-1 in the kidney tissue, and a-SMA positive mesangial cells were observed compared with Fosinopril using western blot, northern blot and immunohistochemistry methods.

对传统的大鼠慢性血清病肾炎模型制作方法进行以下改良：切除大鼠一侧肾脏、腹腔注射大肠杆菌内毒素、以及改尾静脉注射为腹腔注射。与西药福辛普利作对照，观察中药肾华对改良慢性血清病肾炎大鼠尿蛋白、血生化、肾功能、肾脏病理变化的影响。并通过免疫组化、Western蛋白印迹及Northern杂交方法，观察中药肾华对改良慢性血清病肾炎大鼠肾组织肾小球内炎细胞浸润、Stat3蛋白与基因表达及活化、以及其下游调控的TIMP-1蛋白与基因表达、系膜细胞增殖的影响。

Western blot confirmed that the fusion protein reacted to positive serum against sheep pox.

Western blot试验表明，融合蛋白可被羊痘阳性血清识别，这为研究其在新型疫苗和诊断的研究奠定了基础。

The expressed protein accounted for more than 30 % of the total protein mass. Western blot confirmed that the fusion protein reacted to positive serum against sheep pox.

在IPTG 浓度为0.1 mM、温度为37 ℃、诱导6 h时表达量最大，约占菌体蛋白的30% Western blot 试验表明，融合蛋白可被羊痘阳性血清识别，这为研究其在新型疫苗和诊断的研究奠定了基础。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

METHODS The coimmunoprecipitation, sodium dodecyl sulfate polyacry amide gel electrophoresis, peptide mass fingerprint and Western blot analysis were used to isolate and identify the interacting proteins of DNA-PKcs.

通过免疫共沉淀获得DNA-PKcs的相互结合蛋白复合物，SDS-PAGE电泳分离蛋白，肽质指纹分析和Western印迹鉴定蛋白。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。