查询词典 Western

In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection.

本研究采用RT-PCR和Western Blotting技术，检测正常培养和感染HCMV的神经胶质瘤（U251）细胞NGF的表达，探讨人巨细胞病毒对神经胶质瘤细胞内源性神经生长因子表达的影响。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组，术前两天至术后两天，口服Doxycycline，30mg／kg／天)，分别于术后第一天、术后一周、术后两周、术后四周取心肌组织，采用免疫组化测定胶原含量和Ⅰ／Ⅲ胶原比例，酶谱法测定心梗后MMP-2，9活性蛋白的表达规律，Western Blotting进一步确定酶谱法中所消化条带蛋白的属性，逆转录-聚合链反应法测定心梗后MMP-2，9和TIMP-1的mRNA的变化规律，运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

In this paper, the complete nucleotide sequence of watermelon mosaic virus Chinese Isolate was determined. On the basis, the HC-Pro protein and CP protein both are the factors for aphid transmission were cloned and expressed in vitro, and their antiserum were prepared. By far western blot, viral receptors were identified in aphids, and their interactions were studied. The molecular mechanism of nonpersistent transmission was further elucidated.

本文完成了我国西瓜花叶病毒的基因组全序列的测定，在此基础上，克隆和体外表达了蚜传因子HC-Pro和CP蛋白，制备了相应抗体，利用Far western blot技术鉴定了其蚜虫体内的受体蛋白，并对三者互作关系进行研究，进一步阐明了非持久性传毒的分子机制。

TNFR1/RANK chimera cloning of cDNA, with its plat E-retroviral package by retroviral infection such transfer to TNFR1/R2 gene knock-out mice (TNFR1-/R2-) Bone marrow macrophages in; TNFɑ used to stimulate, respectively, to observe the OC and differentiation of bone re-absorption capacity; confirmed by Western Blot method TNFR1/RANK through NF-?

克隆TNFR1/RANK嵌合体的cDNA，用plat E将其包装成逆转录病毒，通过感染将这种逆转录病毒转染到敲除TNFR1/R2基因的小鼠(TNFR1-/R2-)骨髓巨噬细胞中；用TNFɑ刺激，分别观察OC的分化及骨的重吸收能力；用Western Blot方法证实TNFR1/RANK是通过NF-？

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各式各样的剪纸、做工精细的刺绣，还有象征着吉祥如意的中国结……如果你想把它们搬回家，只要选择paypal支付、Western Union Money Transfer/Money Gram支付、Credit Card支付，大约一周以后，你所选购的商品就会被送到你的手上了。

Perceptively on 3d and 5d. Collagen is positive in Western blot on 9d. Aniline dyeing is positive on 12d. The control group was negative. Ad-BMP-2 transferred MSCs cultured in fibrin gel was positive in Aniline dyeing, Immunohistochemical. The therapy effect of group was better in histology, biochemistry and biomechanics in all periods than the other two groups, and was similar to the natural cartilage.

结果 ①Ad-BMP-2转基因MSC BMP-2、CollagenⅡ的PT-PCR分别在3、5d呈阳性，CollagenⅡ的Western Blot 9d为阳性，较对照组有显著差异；②转基因MSC经纤维蛋白体外三维培养后免疫组化和苯胺蓝染色呈阳性，电镜显示细胞生长良好，有基质合成；③转基因修复组各时期大体、组织形态学和组织化学成份均显著优于其它两组，12W时力学和组织学已接近正常关节软骨。

The humanization and specificity of chimeric antibody was identified by ELISA and Western bloting, and the tumoricidal activity of the expressed chimeric antibody was detected by tetrazolium salt phenazine methosulfate assay.

采用ELISA和Western bloting法鉴定抗体的人源性和抗体-抗原结合活性，四唑盐/吩嗪硫酸甲酯比色法检测抗体的生物学活性。

Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

The recombinant protein was purified with the different pH. Western blotting testing of the porcine expression of pGEMX POB in E.coli BL21 was positive.

利用非融合表达产品制备抗血清，检测融合表达的重组蛋白，Western-blot为阳性。

As soon as Bell Labs engineered a producible American magnetron, Western Electric set up to build them.

当贝尔实验室着手设计美国的磁控管时，Western Electric已开始生产了。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。