查询词典 Western

The new antigen P3 peptide which harvested from the 430A peptide synthesizer were verified by the mass spectrograph and HPLC, that the ratio of mass/charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95%. The Western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22KD and 55KD proteins, which were existed in the testis tissue protein of mouse, rat and human.

一、经抗原分子设计，在430A自动肽合成仪上合成的新抗原P3，经质谱仪分析证明其荷质比与理论一致；纯化后，其纯度大于95％；将新抗原与KLH偶连后免疫小鼠，经Western blot证实其诱导的特异性抗体可识别小鼠、大鼠、人睾丸组织中分子量约为22KD和55KD左右的蛋白质。

The expression of MDK was detected by real-time PCR and Western Blot. Results: The retroviral vector pLXSN-MDK was successfully constructed and infected SGC7901 cells,and the MDK gene expression of SGC7901/pLXSN-MDK was up-regulated.

病毒上清感染SGC7901细胞，G418筛选出阳性克隆，经实时定量PCR及Western Blot检测发现SGC7901细胞中MDK mRNA及蛋白表达水平明显上调。

The expressed product was purified by affinity chromatography and identified by Western blot, then determined for enzyme activity by Micrococcus lysodeikticus turbidimetric method and for biological activity by heat shock method.

表达产物经亲和层析纯化后，进行Western blot鉴定，并用溶壁微球菌比浊法检测酶活性，热激法检测其生物学活性。

Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型，分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本，行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况；行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润；行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达；分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot.

重组短穗鱼尾葵花粉profilin在大肠杆菌中高效的表达，进一步经Ni2＋亲和层析柱纯化后经Western-blot检测具有良好的免疫学活性。

Expression of peripheral monocyte MMP-9 was measured by RT-PCR and Western blot.

分离大鼠外周血单核细胞，用RT-PCR和Western blot检测MMP-9表达的变化。

Western Blot was utilized to evaluate the morphism on the expression of cyclin D1, Cdk6 and pRb and phosphorylation of pRb.

Western Blot法检测Cyclin D1基因多态性对Cyclin D1、Cdk6和pRb的表达水平以及对pRb磷酸化水平的影响。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞，获得重组杆状病毒rBV-σC；SDS-PAGE和Western-blot分析表明，在sf-9细胞中表达了分子质量约37ku的σC蛋白，该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应，这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

Western blotting shows that CTGF protein was over-expressed in neurofibroma tissue.

Western blotting结果显示神经纤维瘤组织有明显的CTGF蛋白表达条带。

Methods Specimens of neurofibroma tissue, keloid and normal skin were collected. The distribution and expression of CTGF was measured by immunohistochemistry. The mRNA expression of CTGF, TGF-β1,ColⅠ and ColⅢ was detected by reverse transcription- polymerase chain reaction. Expression of CTGF protein was measured by western blotting.

收集神经纤维瘤组织、瘢痕疙瘩组织及正常皮肤组织标本，免疫组化检测CTGF的表达，逆转录-聚合酶链式反应方法检测组织CTGF、TGF-β1、Ⅰ型胶原及Ⅲ型胶原 mRNA表达，Western blotting检测组织CTGF蛋白的表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。