查询词典 Western

Western Blot was used to test the expression of NF-κB in nuclear and cytoplasm. The intranuclear expression of NF-κB was confirmed by ELISA assay. The influence on cell apoptosis of gastric carcinoma cell by Paeoniflorin and 5-FU was estimated by flow cytometry.

应用Western Blot方法检测各组胞核NF-κB表达情况，并应用胞核NF-κB活性检测进一步确定胞核内NF-κB的表达情况，流式细胞仪检测联合应用芍药苷及5-氟尿嘧啶(5-FU)对胃癌细胞凋亡的影响？

Although many efforts have been made to elucidate the role of Kail gene in many kinds of carcinoma,there are little reports concerning the role of Kail in human colorectal carcinoma,especially regarding the mechanism of its down-regulation in metastasis carcinoma. In present study,the expression of Kail was detected by in situ hybridization,immumohistochemistry and Western Blot in four cell lines of HCC with different metastatic potential.

为探讨Kai1基因与大肠癌转移之间的相关性，本研究利用四种不同转移潜能的大肠癌细胞系HCT116、HT29、LoVo与SW480，从mRNA与蛋白质水平，用原位核酸杂交、免疫组织化学与Western Blot三种方法检测Kai1／CD82的表达，得到了一致的结果：Kai1／CD82在HT29与SW480细胞系的表达较LoVo与HCT116为高。

The results indicated that Kail/CD82 is higher expressed in HT29 and SW480 than in LoVo and HCT116 whatever in mRNA or protein level.The possible mechanism of down regulation of Kail was also analyzed. By PCR-SSCP,8 exons of Kail,exon 3 to exon 10,were screened in LoVo and HCT116 cell lines. The methylation of CpG islands in promoter region was analyzed,and the expression level of p53 was detected by IH and Western Blot.

为进一步探讨Kai1基因表达下调的机制，本研究用PCR-SSCP技术分析了Kai1／CD82表达较低的LoVo与HCT116细胞系的Kai1基因第3～10外显子是否存在点突变，检测Kai1基因启动子区域CpG岛的甲基化状况，并用免疫组化与Western Blot等方法研究Kai1基因表达与p53异常之间的相关性。

Western blotting results showed that the expressed protein had similar immunogenicity with the natural α-BGT protein purified from the venom of Krait Bungarus spp..

经Western blot分析，在大约8 kDa处出现明显的目的带，与预计蛋白分子量大小一致，说明表达产物与天然α-银环蛇毒素具有相似的免疫原性。

The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized the seed toxin protein of Jatropha curcas L. by Western blot.

然后我们以麻疯树的胚乳、根、茎、叶、叶柄及其诱导出的愈伤组织为材料，对麻疯树种子毒蛋白作Western-blot杂交。

At 0, 4, 8, 16, 24 h after operation, the expressions of SR-A protein and mRNA in liver tissue were assayed by western blot and RT-PCR respectively; the levels of plasma endotoxin and interleukin-6 (IL-6) were measured using limulus test and ELISA.

于术后0、4、8、16、24 h分别采用Western blot和RT-PCR检测肝组织中SR-A蛋白和基因表达水平；以鲎试验和ELISA测定血浆内毒素和白介素-6(IL-6)；光镜观察肝组织病理变化并测定血浆ALT、TB含量。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

To 7 days after transduction, cells were observed and recorded with fluorescence microscope, the expression levels of report gene EGFP in tumor cells were examined by using flow cytometry and Western blotting, the expression of report genes luciferase was analyzed with luminometer to obtain the relative light units.

用荧光显微镜观察EGFP阳性细胞，流式细胞仪检测EGFP阳性细胞的百分率和单个细胞平均荧光强度，Western印迹检测EGFP蛋白表达的变化以比较各组病毒转导效率。

After two hours of the mice leaveing warming boxes, 5 mice randomly selected from each group per timesection were executed by cervical vertebra luxation. The HSP70 of embryonic brain, protein content and deformations of embryonic nervous systems were quantitatively analyzed with the Western dot blot and Lowry method.

移出温箱2h后，每组每时段随机取5只脱颈法处死，采用Western dot blot 和lowry方法定量分析胚胎脑HSP70和蛋白质含量与胚胎神经系统畸形。

The lysate of the cells were collected to detect the expression of the two genes by Western blot.

pylori与AGS细胞共同培养1h，用Western blot方法检测细胞内c-Fos和c-Jun基因表达的变化。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。